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Sensors, Volume 2, Issue 4 (April 2002), Pages 127-163

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Research

Open AccessArticle Planar Amperometric Glucose Sensor Based on Glucose Oxidase Immobilized by Chitosan Film on Prussian Blue Layer
Sensors 2002, 2(4), 127-136; doi:10.3390/s20400127
Received: 27 February 2002 / Accepted: 19 March 2002 / Published: 23 April 2002
Cited by 46 | PDF Full-text (118 KB) | HTML Full-text | XML Full-text
Abstract
A planar amperometric glucose microsensor based on glucose oxidase immobilized by chitosan film on Prussian Blue layer has been developed. The experimental results show that the optimum detection potential is 50 mV (versus Ag/AgCl) and the optimum pH is 6.5. Under the selective
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A planar amperometric glucose microsensor based on glucose oxidase immobilized by chitosan film on Prussian Blue layer has been developed. The experimental results show that the optimum detection potential is 50 mV (versus Ag/AgCl) and the optimum pH is 6.5. Under the selective conditions the sensor exhibits excellent sensitivity of 98 nA/M and a linear range of 0.1-6.0 mM. The apparent Michanelis-Menten constant of the sensor is 21 mM. The response time is less than 60 seconds. No apparent change in the response to glucose was observed during one month. Foremost, the interference of ascorbic and uric acids can be avoided due to selective permeability of chitosan film and electrocatalysis of PB layer to H2O2. The sensor has been applied to detect glucose in human blood serum. Full article
Open AccessArticle Asymptotic Regional Boundary Observer in Distributed Parameter Systems via Sensors Structures
Sensors 2002, 2(4), 137-152; doi:10.3390/s20400137
Received: 6 March 2002 / Accepted: 4 April 2002 / Published: 26 April 2002
PDF Full-text (183 KB)
Abstract
The purpose of this paper is to study the concept of asymptotic regional observer in connection with the characterizations of sensors. We give various results related to different types of measurements, of domains and boundary conditions. Furthermore, we show that the measurements structures
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The purpose of this paper is to study the concept of asymptotic regional observer in connection with the characterizations of sensors. We give various results related to different types of measurements, of domains and boundary conditions. Furthermore, we show that the measurements structures allow the existence of regional observer and we give a sufficient condition for such observer. We also show that, there exists a dynamical system for the considered system is not observer in the usual sense, but it may be regional boundary observer. Full article
Open AccessArticle Natural Monocrystalline Pyrite as Sensor for Potentiometric Redox Titrations. Part I. Titrations with Permanganate
Sensors 2002, 2(4), 153-163; doi:10.3390/s20400153
Received: 9 April 2002 / Accepted: 23 April 2002 / Published: 30 April 2002
Cited by 2 | PDF Full-text (94 KB) | HTML Full-text | XML Full-text
Abstract
Results obtained in potentiometric titrations of Fe(II), Mn(II), Fe(CN)64-, C2O42- and As(III) with standard potassium permanganate solution, are presented. The titration end point (TEP) was detected with a universal electrode whose sensor is natural crystalline pyrite.
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Results obtained in potentiometric titrations of Fe(II), Mn(II), Fe(CN)64-, C2O42- and As(III) with standard potassium permanganate solution, are presented. The titration end point (TEP) was detected with a universal electrode whose sensor is natural crystalline pyrite. The titrations of As(III) were carried out in HCl (1.2 M) and H2SO4 solutions (0.1- 4.5 M), whereas oxalate was determined in H2SO4 (0.1-4.5 M). Iron(II) and hexacyanoferrate(II) were titrated in H2SO4 and also in H3PO4 solutions (0.1-4.5 M). The titrations of Mn(II) were performed in H2P2O72- media at pH 4.0, 5.0, 6.0 and 7.0. The results obtained by using the pyrite electrode were compared with those obtained by the application of a Pt-electrode, and good agreement, reproducibility and accuracy were obtained. The potentials in the course of the titration and at the end-point (TEP) are rapidly established. The potential changes at the TEP ranged from 90 to 330 mV/0.1 mL, depending on the titrated system. The highest changes were observed in titrations of Fe(II) in H3PO4 (240-330 mV/0.1 mL). Reversed titrations were also performed and accurate and reproducible results were obtained. Full article

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