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Int. J. Environ. Res. Public Health 2013, 10(7), 2897-2905; doi:10.3390/ijerph10072897
Article

Development of a Monoclonal Antibody-Based Sandwich ELISA for Peanut Allergen Ara h 1 in Food

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State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, China
* Author to whom correspondence should be addressed.
Received: 6 May 2013 / Revised: 17 June 2013 / Accepted: 3 July 2013 / Published: 12 July 2013
(This article belongs to the Special Issue Food Allergy, Genes and Environment)
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Abstract

We have established a highly sensitive sandwich enzyme-linked immunosorbent assay (ELISA) based on two monoclonal antibodies (mAb) to measure the content of the major peanut allergen Ara h 1 in foods. Two mAbs were selected out of 12 murine hybridoma cells secreting Ara h 1-specific antibody. Using mAb 6 as the capture antibody and HRP-labelled mAb 4 as the detection antibody, the limit of detection (LOD) the assay was 0.34 ng/mL. Cross-reaction analysis showed that this method was strongly specific and had no cross-reactions with Ara h 2, pea protein or soy protein. Sample analysis showed that this ELISA was a useful tool to monitor peanut allergens in food products by measuring Ara h 1 content.
Keywords: peanut allergen; Ara h 1; monoclonal antibody; sandwich ELISA peanut allergen; Ara h 1; monoclonal antibody; sandwich ELISA
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Peng, J.; Song, S.; Xu, L.; Ma, W.; Liu, L.; Kuang, H.; Xu, C. Development of a Monoclonal Antibody-Based Sandwich ELISA for Peanut Allergen Ara h 1 in Food. Int. J. Environ. Res. Public Health 2013, 10, 2897-2905.

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