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High-Throughput, Volume 6, Issue 3 (September 2017)

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Editorial

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Open AccessEditorial Change of Title: Microarrays Becomes High-Throughput
High-Throughput 2017, 6(3), 10; doi:10.3390/ht6030010
Received: 17 July 2017 / Accepted: 17 July 2017 / Published: 19 July 2017
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Abstract
MDPI’s journal Microarrays released its first volume in 2012. Since then, the journal has published 129 articles on the topic of microarrays, including their applications, analysis and new developments. [...] Full article

Review

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Open AccessReview Oral Health: The Need for Both Conventional Microbial and Molecular Characterization
High-Throughput 2017, 6(3), 11; doi:10.3390/ht6030011
Received: 10 July 2017 / Revised: 26 July 2017 / Accepted: 27 July 2017 / Published: 1 August 2017
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Abstract
This study aims to consider the microbial distribution in oral disease, as well as gene analysis and expression, in elucidating: 1, the fundamental underpinnings of oral disease, and 2, the potential relationship between oral diseases and systemic health. A key focus is identifying
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This study aims to consider the microbial distribution in oral disease, as well as gene analysis and expression, in elucidating: 1, the fundamental underpinnings of oral disease, and 2, the potential relationship between oral diseases and systemic health. A key focus is identifying the microbiota associated with oral disease manifestations characterized by both conventional microbiological and molecular methods. Variations in the observed microbial populations characterized by conventional and molecular approaches have been identified for caries, periodontitis, peri-implantitis, and stomatitis. The discovery of therapeutic approaches for oral disease will require comprehensive microbial and genomic analysis. This study evaluated the current state of the relevant microbial and genomic information for several prevalent oral diseases. Full article

Other

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Open AccessOpinion High‐Throughput Methods to Detect Long Non‐Coding RNAs
High-Throughput 2017, 6(3), 12; doi:10.3390/ht6030012
Received: 21 August 2017 / Accepted: 29 August 2017 / Published: 31 August 2017
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Abstract
Increasing evidence suggests that the numbers of long non‐coding RNAs (lncRNAs) are more than those of protein‐coding genes in various organisms. Although the detection methods for lncRNAs are being increasingly established, there are advantages and disadvantages that exist for each method. In this
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Increasing evidence suggests that the numbers of long non‐coding RNAs (lncRNAs) are more than those of protein‐coding genes in various organisms. Although the detection methods for lncRNAs are being increasingly established, there are advantages and disadvantages that exist for each method. In this opinion article, I highlight the differences between microarrays and RNA sequencing (RNA‐seq) for the detection of lncRNAs. Compared to RNA‐seq, microarrays are limited to the known sequences. However, the detection method as well as data analysis workflow is more established, which makes it easier to analyze the data for bench scientists without extensive knowledge about computer programming. In order to highlight the usage of microarrays over RNA‐seq for the detection of lncRNAs, we are organizing a special issue for High‐Throughput called “Microarrays in Non‐Coding RNAs Profiling”, which will include the specific usages of microarrays for lncRNAs. Full article

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