Pre-Degassed Microfluidic Chamber-Based Digital PCR Device for Meat Authentication Applications
Abstract
:1. Introduction
2. Materials and Methods
2.1. Principles, Design, and Fabrications of Digital Polymerase Chain Reaction (dPCR) Microfluidic Chip
2.2. dPCR Evaluation and Meat Authentication Tests
2.3. Data Acquisition and Analysis
3. Results
3.1. Evaluation of dPCR Microfluidic Chip
3.2. Applications in Meat Authentication
4. Discussion
4.1. Impact of Sample Loading on the Quantitative Performance
4.2. Performance for Meat Authentication Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
- Kaur, H.; Kumar, R.; Babu, J.N.; Mittal, S. Advances in arsenic biosensor development—A comprehensive review. Biosens. Bioelectron. 2015, 63, 533–545. [Google Scholar] [CrossRef]
- Li, S.; Cui, H.; Yuan, Q.; Wu, J.; Wadhwa, A.; Eda, S.; Jiang, H. AC electrokinetics-enhanced capacitive immunosensor for point-of-care serodiagnosis of infectious diseases. Biosens. Bioelectron. 2014, 51, 437–443. [Google Scholar] [CrossRef]
- Li, S.; Ren, Y.; Cui, H.; Yuan, Q.; Wu, J.; Eda, S.; Jiang, H. Alternating current electrokinetics enhanced in situ capacitive immunoassay. Electrophoresis 2015, 36, 471–474. [Google Scholar] [CrossRef]
- Wang, H.-B.; Ma, L.-H.; Zhang, T.; Huang, K.-C.; Zhao, Y.-D.; Liu, T.-C. Simple and accurate visual detection of single nucleotide polymorphism based on colloidal gold nucleic acid strip biosensor and primer-specific PCR. Anal. Chim. Acta 2020, 1093, 106–114. [Google Scholar] [CrossRef] [PubMed]
- Ahrberg, C.D.; Manz, A.; Chung, B.G. Polymerase chain reaction in microfluidic devices. Lab A Chip 2016, 16, 3866–3884. [Google Scholar] [CrossRef] [Green Version]
- Quan, P.-L.; Sauzade, M.; Brouzes, E. dPCR: A technology review. Sensors 2018, 18, 1271. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Demeke, T.; Dobnik, D. Critical assessment of digital PCR for the detection and quantification of genetically modified organisms. Anal. Bioanal. Chem. 2018, 410, 4039–4050. [Google Scholar] [CrossRef] [Green Version]
- Cui, X.; Wu, L.; Wu, Y.; Zhang, J.; Zhao, Q.; Jing, F.; Yi, L.; Li, G. Fast and robust sample self-digitization for digital PCR. Anal. Chim. Acta 2020, 1107, 127–134. [Google Scholar] [CrossRef] [PubMed]
- Baker, M. Digital PCR hits its stride. Nat. Methods 2012, 9, 541–544. [Google Scholar] [CrossRef]
- Si, H.; Xu, G.; Jing, F.; Sun, P.; Zhao, D.; Wu, D. A multi-volume microfluidic device with no reagent loss for low-cost digital PCR application. Sens. Actuators B Chem. 2020, 318, 128197. [Google Scholar] [CrossRef]
- Xu, G.; Si, H.; Jing, F.; Sun, P.; Zhao, D.; Wu, D. A Double-Deck Self-Digitization Microfluidic Chip for Digital PCR. Micromachines 2020, 11, 1025. [Google Scholar] [CrossRef]
- Wei, C.; Yu, C.; Wu, J.J.; Li, J.; Li, S.; Dai, S.; Li, T. Easy-to-operate fabrication of tapered glass capillaries for microdroplet generation. J. Micromechanics Microeng. 2019, 29, 037001. [Google Scholar] [CrossRef]
- Li, T.; Zhao, L.; Liu, W.; Xu, J.; Wang, J. Simple and reusable off-the-shelf microfluidic devices for the versatile generation of droplets. Lab A Chip 2016, 16, 4718–4724. [Google Scholar] [CrossRef] [PubMed]
- Moon, B.-U.; Abbasi, N.; Jones, S.G.; Hwang, D.K.; Tsai, S.S. Water-in-water droplets by passive microfluidic flow focusing. Anal. Chem. 2016, 88, 3982–3989. [Google Scholar] [CrossRef]
- Eggersdorfer, M.L.; Seybold, H.; Ofner, A.; Weitz, D.A.; Studart, A.R. Wetting controls of droplet formation in step emulsification. Proc. Natl. Acad. Sci. USA 2018, 115, 9479–9484. [Google Scholar] [CrossRef] [Green Version]
- Schuler, F.; Trotter, M.; Geltman, M.; Schwemmer, F.; Wadle, S.; Domínguez-Garrido, E.; López, M.; Cervera-Acedo, C.; Santibáñez, P.; von Stetten, F. Digital droplet PCR on disk. Lab A Chip 2016, 16, 208–216. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Zhu, Q.; Qiu, L.; Yu, B.; Xu, Y.; Gao, Y.; Pan, T.; Tian, Q.; Song, Q.; Jin, W.; Jin, Q.; et al. Digital PCR on an integrated self-priming compartmentalization chip. Lab A Chip 2014, 14, 1176–1185. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Li, T.; Wang, J.; Wang, Z.; Qiao, L.; Liu, R.; Li, S.; Chen, A. Quantitative determination of mutton adulteration with single-copy nuclear genes by real-time PCR. Food Chem. 2021, 344, 128622. [Google Scholar] [CrossRef] [PubMed]
- Bohl, B.; Steger, R.; Zengerle, R.; Koltay, P. Multi-layer SU-8 lift-off technology for microfluidic devices. J. Micromechanics Microeng. 2005, 15, 1125. [Google Scholar] [CrossRef]
- Liang, D.Y.; Tentori, A.M.; Dimov, I.K.; Lee, L.P. Systematic characterization of degas-driven flow for poly (dimethylsiloxane) microfluidic devices. Biomicrofluidics 2011, 5, 024108. [Google Scholar] [CrossRef] [Green Version]
- Yeh, E.-C.; Fu, C.-C.; Hu, L.; Thakur, R.; Feng, J.; Lee, L.P. Self-powered integrated microfluidic point-of-care low-cost enabling (SIMPLE) chip. Sci. Adv. 2017, 3, e1501645. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Hindson, C.M.; Chevillet, J.R.; Briggs, H.A.; Gallichotte, E.N.; Ruf, I.K.; Hindson, B.J.; Vessella, R.L.; Tewari, M. Absolute quantification by droplet digital PCR versus analog real-time PCR. Nat. Methods 2013, 10, 1003–1005. [Google Scholar] [CrossRef] [PubMed]
- Liu, G.Q.; Luo, J.X.; Xu, W.L.; Li, C.D.; Guo, Y.S.; Guo, L. Improved triplex real—time PCR with endogenous control for synchronous identification of DNA from chicken, duck, and goose meat. Food Sci. Nutr. 2021, 9, 3130–3141. [Google Scholar] [CrossRef]
Category | Chicken Mass | Mutton Mass | Mass Ratio |
---|---|---|---|
control 1 | 10 g | / 1 | / |
control 2 | / | 10 g | / |
sample 1 | 10 g | 10 g | 1:1 |
sample 2 | 10 g | 100 g | 1:10 |
sample 3 | 1 g | 100 g | 1:100 |
sample 4 | 1 g | 1000 g | 1:1000 |
sample 5 | 100 mg | 1000 g | 1:10000 |
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. |
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Share and Cite
Hu, H.; Cheng, J.; Wei, C.; Li, S.; Yu, C.; Meng, X.; Li, J. Pre-Degassed Microfluidic Chamber-Based Digital PCR Device for Meat Authentication Applications. Micromachines 2021, 12, 694. https://doi.org/10.3390/mi12060694
Hu H, Cheng J, Wei C, Li S, Yu C, Meng X, Li J. Pre-Degassed Microfluidic Chamber-Based Digital PCR Device for Meat Authentication Applications. Micromachines. 2021; 12(6):694. https://doi.org/10.3390/mi12060694
Chicago/Turabian StyleHu, Hezhi, Jingmeng Cheng, Chunyang Wei, Shanshan Li, Chengzhuang Yu, Xiaoshuai Meng, and Junwei Li. 2021. "Pre-Degassed Microfluidic Chamber-Based Digital PCR Device for Meat Authentication Applications" Micromachines 12, no. 6: 694. https://doi.org/10.3390/mi12060694