A Simple Interfacial Platform for Homogeneous Electrochemical Immunoassays Using a Poly(Vinylimidazole)-Modified Electrode
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Reagents
2.2. Apparatus and Electrodes
2.3. Immobilization of PVI and Nickel(II) Ions on ITO Electrode
2.4. Optimization of Electrode Modification Procedures
2.5. Electrochemical Measurements
3. Results and Discussion
3.1. Morphology of PVI-Electro-Polymerized ITO Electrode
3.2. Optimization of Ni-Modified PVI-ITO Electrode Fabrication
3.3. Electrochemical Characterization of Nickel(II) Ions Modified PVI-ITO Electrode
3.4. Immune Reaction between Anti-HA and Fe-HA
3.5. Competition with HA and Fe-HA
3.6. Electrochemical Detection of HA-Spiked Urine Samples
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
Appendix A
Concentration of HA (mg/mL) | This Sensor Method (mg/mL) | HPLC (ppm) | RSD (%) |
---|---|---|---|
0.5 | 0.49 ± 0.024 | 499.74 | 1.75 |
1.0 | 0.98 ± 0.049 | 999.25 | 1.93 |
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Reference | Concentration of Antigen (HA) (mg/mL) | Mediator | Amount of Mediator (mg/mL) | Urine Test (mg/mL) |
---|---|---|---|---|
Our study | 0.0001 mg/mL | Fe(CN)5(amp-HA) | 0.02276 | 0.001 |
[18] | 0.01 mg/mL | Fe(CN)5(amp-HA) | 2.276 | N/A* |
[31] | 0.01 mg/mL | Os(dmo-bpy)2(amp-HA)Cl | 0.015 | 0.01 |
[17] | 0.001 mg/mL | Fe(CN)5(amp-HA) | 0.15 | 0.001 |
[28] | 0.1 mg/mL | Os(phen)2(amp-HA)Cl | 1.0 | 0.1 |
[30] | 0.1 mg/mL | Os(dme-bpy)2(amp-HA)Cl | 2.0 | N/A* |
[36] | 10 mg/mL | Ferrocence-HA-Lysine | 1.0 | 10 |
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Choi, Y.-B.; Jeon, W.-Y.; Kim, H.-H. A Simple Interfacial Platform for Homogeneous Electrochemical Immunoassays Using a Poly(Vinylimidazole)-Modified Electrode. Sensors 2017, 17, 54. https://doi.org/10.3390/s17010054
Choi Y-B, Jeon W-Y, Kim H-H. A Simple Interfacial Platform for Homogeneous Electrochemical Immunoassays Using a Poly(Vinylimidazole)-Modified Electrode. Sensors. 2017; 17(1):54. https://doi.org/10.3390/s17010054
Chicago/Turabian StyleChoi, Young-Bong, Won-Yong Jeon, and Hyug-Han Kim. 2017. "A Simple Interfacial Platform for Homogeneous Electrochemical Immunoassays Using a Poly(Vinylimidazole)-Modified Electrode" Sensors 17, no. 1: 54. https://doi.org/10.3390/s17010054