Synthesis and Characterization of Polyaniline/Graphene Composite Nanofiber and Its Application as an Electrochemical DNA Biosensor for the Detection of Mycobacterium tuberculosis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
- Probe DNA-single stranded DNA (ssDNA):5′-CTC gTC CAg CgC CgC TTC gg-3′
- Complementary target DNA:5′-CCg AAg Cgg CgC Tgg ACg Ag-3′
- Non-complementary DNA:5′-TTT GGT ATT ATT GTT CAT GT-3′.
2.2. Synthesis of PANI and PANI/GP Nanofiber
2.3. Fabrication of DNA Biosensor
2.4. Electrochemical Measurements
2.5. Characterization
3. Results and Discussion
Electrochemical Characterization of the DNA Biosensor
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Mohamad, F.S.; Mat Zaid, M.H.; Abdullah, J.; Zawawi, R.M.; Lim, H.N.; Sulaiman, Y.; Abdul Rahman, N. Synthesis and Characterization of Polyaniline/Graphene Composite Nanofiber and Its Application as an Electrochemical DNA Biosensor for the Detection of Mycobacterium tuberculosis. Sensors 2017, 17, 2789. https://doi.org/10.3390/s17122789
Mohamad FS, Mat Zaid MH, Abdullah J, Zawawi RM, Lim HN, Sulaiman Y, Abdul Rahman N. Synthesis and Characterization of Polyaniline/Graphene Composite Nanofiber and Its Application as an Electrochemical DNA Biosensor for the Detection of Mycobacterium tuberculosis. Sensors. 2017; 17(12):2789. https://doi.org/10.3390/s17122789
Chicago/Turabian StyleMohamad, Fatimah Syahidah, Mohd Hazani Mat Zaid, Jaafar Abdullah, Ruzniza Mohd Zawawi, Hong Ngee Lim, Yusran Sulaiman, and Norizah Abdul Rahman. 2017. "Synthesis and Characterization of Polyaniline/Graphene Composite Nanofiber and Its Application as an Electrochemical DNA Biosensor for the Detection of Mycobacterium tuberculosis" Sensors 17, no. 12: 2789. https://doi.org/10.3390/s17122789