Next Article in Journal
SDN Controller Placement in IoT Networks: An Optimized Submodularity-Based Approach
Next Article in Special Issue
Light-addressable Electrodes for Dynamic and Flexible Addressing of Biological Systems and Electrochemical Reactions
Previous Article in Journal
Feasibility Study of Enhancing Microwave Brain Imaging Using Metamaterials
Previous Article in Special Issue
A LAPS-Based Differential Sensor for Parallelized Metabolism Monitoring of Various Bacteria
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Sensors
Volume 19
Issue 24
10.3390/s19245473
sensors-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Label-Free Detection of E. coli O157:H7 DNA Using Light-Addressable Potentiometric Sensors with Highly Oriented ZnO Nanorod Arrays

by
Yulan Tian
1,†,
Tao Liang
2,†,
Ping Zhu
1,
Yating Chen
1,
Wei Chen
1,
Liping Du
1,
Chunsheng Wu
1,* and
Ping Wang
2,*
1
Institute of Medical Engineering, Department of Biophysics, School of Basic Medical Sciences, Xi’an Jiaotong University, Xi’an 710061, China
2
Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally.
Sensors 2019, 19(24), 5473; https://doi.org/10.3390/s19245473
Submission received: 30 September 2019 / Revised: 7 December 2019 / Accepted: 9 December 2019 / Published: 12 December 2019
(This article belongs to the Special Issue Light-Addressing and Chemical Imaging Technologies for Electrochemical Sensing)
Browse Figures
Versions Notes

Abstract

:
The detection of bacterial deoxyribonucleic acid (DNA) is of great significance in the quality control of food and water. In this study, a light-addressable potentiometric sensor (LAPS) deposited with highly oriented ZnO nanorod arrays (NRAs) was used for the label-free detection of single-stranded bacterial DNA (ssDNA). A functional, sensitive surface for the detection of Escherichia coli (E. coli) O157:H7 DNA was prepared by the covalent immobilization of the specific probe single-stranded DNA (ssDNA) on the LAPS surface. The functional surface was exposed to solutions containing the target E. coli ssDNA molecules, which allowed for the hybridization of the target ssDNA with the probe ssDNA. The surface charge changes induced by the hybridization of the probe ssDNA with the target E. coli ssDNA were monitored using LAPS measurements in a label-free manner. The results indicate that distinct signal changes can be registered and recorded to detect the target E. coli ssDNA. The lower detection limit of the target ssDNA corresponded to 1.0 × 102 colony forming units (CFUs)/mL of E. coli O157:H7 cells. All the results demonstrate that this DNA biosensor, based on the electrostatic detection of ssDNA, provides a novel approach for the sensitive and effective detection of bacterial DNA, which has promising prospects and potential applications in the quality control of food and water.

1. Introduction

The detection of bacterial deoxyribonucleic acid (DNA) would have broad technological implications in areas ranging from biomedicine and the food industry, to environmental control [1,2,3,4]. For instance, the detection of Escherichia coli (E. coli) DNA is of great significance to the quality control of food and water. E. coli O157:H7 is one of the most dangerous foodborne pathogens, occurring in a variety of foods and water; it is able to cause hemorrhagic colitis, and leads to various symptoms, such as bloody diarrhea [5,6,7,8]. It is highly desirable to develop novel approaches for the detection of bacterial DNA in a label-free and cost-effective manner. For this reason, DNA biosensors have attracted increasing attention for their potential use in the label-free detection of bacterial DNA molecules. The cutting edge of the development of DNA biosensors is currently based on labelling strategies, that enable signal transductions and enhance the sensors’ sensitivity [9,10,11,12]. However, these approaches, while promising, suffer from inherent limitations that severely restrict their applicability. Firstly, labelling strategies require some sort of label, which increases the time and cost of DNA detection. Secondly, the labelling process can complicate the DNA-device fabrication and the DNA detection process. Furthermore, the setup of labeling approaches often requires expensive and huge instruments that limit their practical applications, especially in-field applications.
Recent progress in pushing the limits mentioned above by using label-free strategies has proven to be an alternative emerging approach for DNA detection [13,14]. The fast development of micro- and nanofabricated devices has opened up an exciting realm for the development of a new generation of label-free DNA biosensors. For instance, the label-free detection of E. coli O157:H7 DNA has been reported using piezoelectric sensors and electrochemical biosensors [5,15,16,17]. More recently, field-effect devices (FEDs) have offered a promising direct electrical readout for label-free DNA detection [18,19,20,21]. DNA-FEDs detect the intrinsic molecular charges of DNA molecules. These devices are unrelated to any labelling for signal transduction. They have shown tremendous promise for label-free DNA detection in a much faster, more efficient manner. The basic mechanism is based on the electrical detection of surface-charge changes caused by the hybridization of probe single-stranded DNA (ssDNA) molecules (immobilized on the sensor’s surface) with complementary target ssDNA molecules on the gate surface of the FEDs. As a result, the vast majority of DNA-FEDs are developed for the detection of ssDNA molecules. In this context, little is known about using FEDs for the direct label-free detection of bacterial ssDNA, which is a common and challenging task in many applications, such as the quality control of food and water [22,23].
The light-addressable potentiometric sensor (LAPS) is a type of FEDs. It has a light-addressable gate surface and is suitable for use as a transducer for the label-free electrical detection of DNA molecules based on their intrinsic molecular charges [21,24]. ZnO nanorods have shown promising potential in many applications, including sensors, due to their properties of having a direct wide bandgap, large exciton binding energy, and a high aspect ratio [25]. ZnO nanorods have the advantage of being highly oriented, well-structured, and being easy to prepare on substrate surfaces [26,27,28]. In addition, ZnO NRAs allow the loading of more functional sensing molecules due to their enlarged special surface area, which could potentially improve the sensing capability of the biosensors. Hence, it is essential to explore the feasibility of LAPS using ZnO NRA deposits for the direct label-free detection of bacterial DNA.
Inspired by this idea, a LAPS with ZnO NRA deposits, which is a typical FED with flexible gate electrodes, was used for the direct label-free electrostatic detection of negatively charged bacterial ssDNA molecules using their intrinsic molecular charges for the first time. The probe ssDNA molecules were covalently attached onto the LAPS surface via the silanization process. ZnO NRAs deposited on the LAPS surface could enlarge the surface area necessary for the probe ssDNA immobilization and provide three-dimensional (3D) sites for the probe ssDNA molecules to hybridize more effectively with the target ssDNA. The hybridization of the probe ssDNA with the target E. coli O157:H7 ssDNA was measured by recording the shifts in the LAPS photocurrent–voltage (I–V) curves. It is worth noting that the LAPS chips deposited with ZnO NRAs and bacterial ssDNA sequences used in this study only demonstrate the technical feasibility of this novel approach for direct bacterial ssDNA sensing.

2. Materials and Methods

2.1. Fabrication and Functionalization of LAPS Chip

A LAPS chip was fabricated using a p-doped silicon wafer with a thickness of 400 µm (<100>, 1–10 Ωcm). A 30 nm SiO2 layer was developed on the surface of the silicon wafer through thermal oxidation. To create an Ohmic contact and light illumination, a 300 nm Al layer patterned with a window was fabricated on the rear side of the silicon wafer after etching the rear side of the SiO2 layer. The hydrothermal method was employed to prepare a layer of ZnO NRAs on the surface of the LAPS chip as previous reported [26]. Briefly, a thin layer of ZnO film was deposited on the surface of the LAPS chip via reactive magnetron sputtering, which was used as the seeding layer. The hydrothermal deposition was performed in a solution with equal volumes of Zn(NO3)2 and methenamine at the same concentration of 0.02 M in a sealed beaker. The surface on which the ZnO NRAs were expected to grow was put downward at 95 °C for 2 h. Then, the LAPS chip was thoroughly rinsed with deionized water, and blown dry with N2 for structure characterization and further sensing experiments.
The sequence of the probe ssDNA was designed specifically for the E. coli O157:H7 eaeA gene, which is a 30-base oligonucleotide (5’-AACGC CGATA CCATT ACTTA TACCG CGACG-3’). The sequences of the fully mismatched ssDNA were 5’-GCAGC GCCAT ATTCA TTACC ATAGC CGCAA-3’, which contains a fully mismatched base sequence to the probe ssDNA. Both the probe ssDNA and the fully mismatched ssDNA were synthesized by the Takara Biotechnology Company, Limited. To avoid any influence caused by solution changes on the measurement, 10 mM of phosphate-buffered saline (PBS) (pH 7.5) was used as the measurement solution for all the measurements and preparation of the probe ssDNA solutions. Next, 5′-end amino-modified probe ssDNA molecules were covalently immobilized on the LAPS surface via the silanization process. Briefly, the LAPS surface, deposited with ZnO NRAs, was treated with 0.1% (v/v) 3-aminopropyltriethoxysilane (APTES) in toluene with 1 h of incubation at room temperature (RT). Then, the LAPS surface was rinsed with the solvent and blown dry using N2, then heated for 2 h at 120 °C to solidify the attachment. The introduced amine residues reacted with the added glutaraldehyde overnight at room temperature. The introduced aldehyde residues reacted with the amine residues on the end of the probe ssDNA at RT for 12 h. Finally, a 1% bovine serum albumin (BSA) was added to block any unreacted aldehyde residues and any other non-specific binding sites on the ZnO NRAs. The sensor was rinsed with PBS and stored at 4 °C for further experiments.

2.2. Preparation of Target E. coli O157:H7 ssDNA

The target ssDNA with part of the base sequence complementary to the probe ssDNA was prepared from the E. coli O157:H7 eaeA gene using an asymmetric polymerase chain reaction (PCR). First, the E. coli O157:H7 were cultured in a nutrient broth at 37°C for 12 h, then it was killed using a 100 °C water bath for 15 min. Then, the E. coli O157:H7 was serially diluted to the desired concentrations with PBS by the surface plating-count method. The E. coli O157:H7 genomic DNA was extracted using an E.N.Z.A Bacterial DNA Kit (Beijing Solarbio Science & Technology Co. Ltd, China) following the product’s instructions and used as the DNA template for the asymmetric PCR in order to achieve the target E. coli O157:H7 ssDNA for label-free DNA detection, based on the hybridization of the target ssDNA with the probe ssDNA. The forward and reverse primers specific to the E. coli O157:H7 eaeA gene were 5’-GGCGG ATAAG ACTTC GGCTA-3’ and 5’-CGTTT TGGCA CTATT TGCCC-3’, respectively. For asymmetric PCR, the concentration of reverse primers was set to be 50 times higher than that of the forward primers. As a result, the forward primers played the role of a “limiting primer” (i.e., the target E. coli O157:H7 eaeA gene ssDNA will be generated by the reverse primer after the limiting forward primer was consumed). The asymmetric PCR reaction was carried out using a Bio-Rad Thermal Cycler (Bio-Rad Laboratories, Inc., Hercules, CA, USA) under the following conditions: 95 °C for 5 min preincubation, followed by 38 cycles of 30 sec denaturation at 95 °C, 30 sec of annealing at 55 °C, a 45 sec extension at 72 °C, and a 10 sec final extension. The product of the asymmetric PCR was a short ssDNA fragment (151 bases) of the E. coli O157:H7 eaeA gene, which contained the complementary base sequence to the probe ssDNA, thus allowing for the hybridization of the probe ssDNA and the target ssDNA on the LAPS surface for the detection of E. coli O157:H7 ssDNA.

2.3. LAPS Measurement Setup and Target ssDNA Detection

Figure 1 is a schematic diagram of the LAPS measurement setup used in this study, which is similar to those in our previous reports [29]. The light source was an He–Ne semiconductor laser (Coherent Co., Santa Clara, CA, USA) with a wavelength of 543.5 nm and a diameter of 1 mm for illuminating the local area on the LAPS chip. A potentiostat (EG & G Princeton Applied Research, M273A, USA) was used to provide bias voltage; this was applied to the LAPS chip for the generation of photocurrent during illumination. A lock-in amplifier (model SR830 DSP, Stanford Research Systems) was utilized to amplify the photocurrent of the LAPS chip. A National Instruments data-acquisition card (DAQmx PCI-6259, National Instruments, TX, USA) was employed to record and collect the amplified photocurrent. Home-made LabVIEW software was used to control the whole measurement setup. All measurements were carried out at RT. The whole setup was shielded with a Faraday box to exclude ambient light and to minimize the influences of environmental factors on the measurements.
The LAPS surface charges changed after the probe ssDNA hybridized with the target ssDNA on its surface, which can be detected by recording the shifts of the LAPS photocurrent–voltage curves (I–V curves). First, the measurement solution (10 mM PBS, pH 7.5) was added to the detection chamber to record the I–V curves from the LAPS chip functionalized with the probe ssDNA. Then, the measurement solution in the detection chamber was removed and incubated with a solution containing the target E. coli O157:H7 ssDNA for 1 h at RT. Then, the measurement solution was used to wash the detection chamber, to remove any ssDNA molecules that did not hybridize with the probe ssDNA on the LAPS surface. Finally, the I–V curves were recorded from the LAPS surface after the DNA hybridization. The charge changes induced by the target ssDNA hybridization with the probe ssDNA on the LAPS surface can be indicated by a comparison of the I–V curves recorded from the LAPS before and after the target ssDNA hybridization. The shifts of the I–V curves were calculated and used as an indicator of the target ssDNA hybridization that occurred on the LAPS surface functionalized with the probe ssDNA.

3. Results and Discussion

3.1. Probe ssDNA Immobilization on the LAPS Surface

The surface of the LAPS chip used in this study was deposited with a layer of ZnO NRAs, which allowed us to load more probe ssDNA molecules due to their enlarged special surface area. A silanization process was performed to treat the LAPS surface and covalently immobilize the probe ssDNA on the sensor surface. A scanning electron microscope (SEM) was employed to characterize the ZnO NRAs deposited on the LAPS surface before and after silanization. Figure 2a shows the SEM image of the ZnO NRAs deposited on the LAPS surface before silanization. It can be observed that the orientation of the ZnO NRAs on the LAPS surface is highly ordered. The diameters of the ZnO NRAs are close to 50 nm, and their lengths and the space between the rods are about 500 nm and 100 nm, respectively. This geometry provided an ideal surface for the loading of more probe ssDNA. Figure 2b shows the SEM images of ZnO NRAs after silanization. It reveals that, with the treatment of 5% APTES, the attachment of APTES residues is apparent on the surface of the nanorods, thus providing sites for the further crosslink of the probe ssDNA using glutaraldehyde as bridges. Furthermore, after the silanization with APTES, the nanorods retained the same morphology, through which the probe ssDNA molecules could diffuse into and subsequently immobilize on the surface of the nanorods through a condensation reaction.
For the detection of the target E. coli O157:H7 ssDNA, it was necessary to functionalize the LAPS surface with the probe ssDNA, which contained a specific complementary base sequence to the target ssDNA. BSA blocking is also desirable to avoid the non-specific adsorption of molecules on the sensor surface. The LAPS surface modifications mentioned above can lead to shifts in the LAPS I–V curves. It is due to the surface charge changes originated from the attachment of charged molecules on the LAPS surface. Figure 3a clearly shows that both the probe ssDNA immobilization and the BSA blocking-induced shifts of the LAPS I–V curves to the positive direction of bias voltage. This is mainly due to the adsorption of the negatively charged probe ssDNA and BSA molecules on to the LAPS surface. In addition, the optimal concentration of probe ssDNA was found to be 5 μM, as indicated by the highest shifts of the LAPS I–V curves. This reflects that the probe ssDNA molecules are immobilized on the LAPS surface with greater efficiency. As compared to the LAPS chip without the ZnO NRAs, significant higher shifts of the LAPS I–V curves can be induced by probe ssDNA immobilization (Figure 2b). This proves our hypothesis that the LAPS chip with ZnO NRAs is able to load more probe ssDNA. This could improve the capability of the sensing target ssDNA, especially in increasing the sensor dynamic range. On the other hand, with regard to the shifts of LAPS I–V curves induced by BSA blocking, no obvious difference was found between the LAPS chips with and without ZnO NRAs (Figure 2b). All the results demonstrated that, compared to the LAPS chip without ZnO NRAs, the LAPS chip with ZnO NRAs was able to load more probe ssDNA molecules, and this could potentially enhance the detection of the target ssDNA.

3.2. Dectection of Target E. coli O157:H7 ssDNA

After the probe ssDNA immobilization and BSA blocking, the prepared DNA biosensor was utilized for the detection of the target ssDNA amplified using an asymmetric PCR from different concentrations of E. coli O157:H7, ranging from 10 CFU/mL to 105 CFU/mL. Asymmetric PCR makes the PCR product become ssDNA, which makes it possible to hybridize it with the probe ssDNA, thus allowing it to be detected directly by the DNA biosensor. Moreover, the DNA concentrations of asymmetric PCR products are proportional to the concentrations of E. coli O157:H7. The proportional constant between the concentrations of target DNA molecules and E. coli O157:H7 was estimated to be 2.75 × 1011 according to the thermal cycles used in the protocol of asymmetric PCR. Therefore, the target ssDNA concentrations corresponding to 10 CFU/mL and 105 CFU/mL E. coli O157:H7 were estimated to be 4.57 nM and 45.7 μM, respectively. The measurement results indicate that asymmetric PCR products lead to the shifting of LAPS I–V curves to the more positive direction of bias voltage (Figure 4a). This is mainly attributed to the hybridization of the target ssDNA molecules with the probe ssDNA immobilized on the LAPS surface, which introduces more negative charges to the sensor surface, due to the molecules’ intrinsic negative charges. In addition, more shifts of the LAPS I–V curves were observed to the positive direction of bias voltage when the asymmetric PCR products from higher concentrations of E. coli O157:H7 were applied for measurement. The shift of the LAPS I–V curves caused by the asymmetric PCR products from concentrations lower than 100 CFU/mL of E. coli O157:H7 were negligible, which indicates that the detection limit of this DNA biosensor for the detection of E. coli O157:H7 was as low as 100 CFU/mL.
The LAPS chip without ZnO NRAs was functionalized with the probe ssDNA and employed as a comparison to show the influences of ZnO NRAs on the performance of this DNA biosensor. As shown by the comparison in Figure 4b, it is obvious that the LAPS chip with ZnO NRAs showed a higher detection capability for E. coli O157:H7, as indicated by the higher slope of responsive linear curves. The detection limit of the LAPS chip without ZnO NRAs is 500 CFU/mL of E. coli O157:H7. This is probably because more probe ssDNA was attached to the LAPS chip with ZnO NRAs compared to the LAPS chip without ZnO NRAs. In addition, ZnO NRAs could provide 3D sites for the probe ssDNA molecules to hybridize with the target ssDNA more effectively. On the other hand, fully mismatched ssDNA alone was applied to test the specificity of this DNA biosensor. The results show that the fully mismatched ssDNA only induced negligible shifts in the LAPS I–V curves. This demonstrated a good specificity for the detection of target ssDNA amplified from the E. coli O157:H7.
However, since this DNA biosensor can only respond to target ssDNA, it is necessary to amplify the target ssDNA from E. coli O157:H7 using asymmetric PCR, which makes it impossible for this biosensor to detect E. coli O157:H7 directly from real samples. In further work, we will focus on the integration of sample preparation and treatment unit in this DNA biosensor, which could allow for the direct detection of real samples of E. coli O157:H7.

4. Conclusions

In this study, we have demonstrated that, with ZnO NRAs deposited on the surface of a LAPS, the hybridization of the target ssDNA amplified from E. coli O157:H7—with the probe ssDNA immobilized on the ZnO NRAs—can be detected by the shift of the I–V curve on the LAPS readout. Compared to the LAPS chip without ZnO NRAs, the sensing capability of DNA biosensors using a LAPS chip with ZnO NRAs improved significantly, as indicated by the higher responsive signals and lower detection limit. This improvement is mainly attributed to the dimensional and sensing properties of ZnO NRAs. In the near future, we will focus on the direct detection of ssDNA without amplification by integrating functional units to this DNA biosensor, which has great potential for the development of portable instruments toward the in-field detection of bacteria. With its ability to detect the hybridization of DNA molecules and its light-addressable characteristics, LAPS is a potential candidate for a new kind of label-free addressable DNA microarray and DNA chip, which has the advantages of saving time and decreasing the cost of potential applications in many fields, such as biomedicine, food and water quality control, and individualized medicine.

Author Contributions

Conceptualization, C.W. and P.W.; methodology, T.L. and Y.T.; software, Y.T.; validation, Y.C., P.Z. and T.L.; formal analysis, P.Z.; investigation, T.L.; resources, P.W.; data curation, W.C. and L.D.; writing—original draft preparation, Y.T.; writing—review and editing, C.W.; supervision, C.W. and P.W.; project administration, C.W. and P.W.; funding acquisition, L.D., C.W. and P.W.

Funding

This research was funded by the National Natural Science Foundation of China, China (Grant Nos. 51861145307, 31700859, and 31661143030), the Doctoral Fund of Education Ministry of China, China (Grant No. 2018M633524), and the Fundamental Research Funds for the Central Universities, China.

Conflicts of Interest

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

References

  1. Teles, F.R.R.; Fonseca, L.P. Trends in DNA biosensors. Talanta 2008, 77, 606–623. [Google Scholar] [CrossRef]
  2. Sassolas, A.; Leca-Bouvier, B.D.; Blum, L.J. DNA biosensors and microarrays. Chem. Rev. 2008, 108, 109–139. [Google Scholar] [CrossRef] [PubMed]
  3. Zhao, W.W.; Xu, J.J.; Chen, H.Y. Photoelectrochemical DNA Biosensors. Chem. Rev. 2014, 114, 7421–7441. [Google Scholar] [CrossRef] [PubMed]
  4. Zulkifli, S.N.; Rahim, H.A.; Lau, W.J. Detection of contaminants in water supply: A review on state-of-the-art monitoring technologies and their applications. Sens. Actuators B Chem. 2018, 255, 2657–2689. [Google Scholar] [CrossRef]
  5. Wang, L.J.; Liu, Q.J.; Hu, Z.Y.; Zhang, Y.F.; Wu, C.S.; Yang, M.; Wang, P. A novel electrochemical biosensor based on dynamic polymerase-extending hybridization for E. coli O157:H7 DNA detection. Talanta 2009, 78, 647–652. [Google Scholar] [CrossRef]
  6. Ariffin, E.Y.; Lee, Y.H.; Futra, D.; Tan, L.L.; Abd Karim, N.H.; Ibrahim, N.N.N.; Ahmad, A. An ultrasensitive hollow-silica-based biosensor for pathogenic Escherichia coli DNA detection. Anal. Bioanal. Chem. 2018, 410, 2363–2375. [Google Scholar] [CrossRef]
  7. Ye, W.W.; Chen, T.; Mao, Y.J.; Tian, F.; Sun, P.L.; Yang, M. The effect of pore size in an ultrasensitive DNA sandwich-hybridization assay for the Escherichia coli O157:H7 gene based on the use of a nanoporous alumina membrane. Microchim. Acta 2017, 184, 4835–4844. [Google Scholar] [CrossRef]
  8. Chan, K.Y.; Ye, W.W.; Zhang, Y.; Xiao, L.D.; Leung, P.H.M.; Li, Y.; Yang, M. Ultrasensitive detection of E. coli O157:H7 with biofunctional magnetic bead concentration via nanoporous membrane based electrochemical immunosensor. Biosens. Bioelectron. 2013, 41, 532–537. [Google Scholar] [CrossRef]
  9. Chen, Y.; Wang, Q.; Xu, J.; Xiang, Y.; Yuan, R.; Cha, Y.Q. A new hybrid signal amplification strategy for ultrasensitive electrochemical detection of DNA based on enzyme-assisted target recycling and DNA supersandwich assemblies. Chem. Commun. 2013, 49, 2052–2054. [Google Scholar] [CrossRef]
  10. Hu, R.; Liu, T.; Zhang, X.B.; Huan, S.Y.; Wu, C.C.; Fu, T.; Tan, W.H. Multicolor Fluorescent Biosensor for Multiplexed Detection of DNA. Anal. Chem. 2014, 86, 5009–5016. [Google Scholar] [CrossRef]
  11. Golub, E.; Niazov, A.; Freeman, R.; Zatsepin, M.; Willner, I. Photoelectrochemical Biosensors Without External Irradiation: Probing Enzyme Activities and DNA Sensing Using Hemin/G-Quadruplex-Stimulated Chemiluminescence Resonance Energy Transfer (CRET) Generation of Photocurrents. J. Phys. Chem. C 2012, 116, 13827–13834. [Google Scholar] [CrossRef]
  12. Zhang, C.Y.; Yeh, H.C.; Kuroki, M.T.; Wang, T.H. Single-quantum-dot-based DNA nanosensor. Nat. Mater. 2005, 4, 826–831. [Google Scholar] [CrossRef] [PubMed]
  13. Ngo, H.T.; Wang, H.N.; Fales, A.M.; Vo-Dinh, T. Label-Free DNA Biosensor Based on SERS Molecular Sentinel on Nanowave Chip. Anal. Chem. 2013, 85, 6378–6383. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  14. Mertens, J.; Rogero, C.; Calleja, M.; Ramos, D.; Martín-Gago, J.A.; Briones, C.; Tamayo, J. Label-free detection of DNA hybridization based on hydration-induced tension in nucleic acid films. Nat. Nanotechnol. 2008, 3, 301–307. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  15. Wang, L.J.; Wei, Q.S.; Wu, C.S.; Hu, Z.Y.; Ji, J.; Wang, P. The E. coli O157:H7 DNA detection on a gold nanoparticle enhanced piezoelectric biosensor. Chin. Sci. Bull. 2008, 53, 1175–1184. [Google Scholar] [CrossRef] [Green Version]
  16. Minaei, M.E.; Saadati, M.; Najafi, M.; Honari, H. Label-free, PCR-free DNA Hybridization Detection of Escherichia coli O157:H7 Based on Electrochemical Nanobiosensor. Electroanalysis 2016, 28, 2582–2589. [Google Scholar] [CrossRef]
  17. Bahşi, Z.B.; Büyükaksoy, A.; Ölmezcan, S.M.; Şimşek, F.; Aslan, M.H.; Oral, A.Y. A Novel Label-Free Optical Biosensor Using Synthetic Oligonucleotides from E. coli O157:H7: Elementary Sensitivity Tests. Sensors 2009, 9, 4890–4900. [Google Scholar] [CrossRef]
  18. Abouzar, M.H.; Poghossian, A.; Pedraza, A.M.; Gandhi, D.; Ingebrandt, S.; Moritz, W.; Schoening, M.J. An array of field-effect nanoplate SOI capacitors for (bio-) chemical sensing. Biosens. Bioelectron. 2011, 26, 3023–3028. [Google Scholar] [CrossRef]
  19. Sorgenfrei, S.; Chiu, C.Y.; Gonzalez, R.L.; Yu, Y.J.; Kim, P.; Nuckolls, C.; Shepard, K.L. Label-free single-molecule detection of DNA-hybridization kinetics with a carbon nanotube field-effect transistor. Nat. Nanotechnol. 2011, 6, 126–132. [Google Scholar] [CrossRef] [Green Version]
  20. Stine, R.; Robinson, J.T.; Sheehan, P.E.; Tamanaha, C.R. Real-Time DNA Detection Using Reduced Graphene Oxide Field Effect Transistors. Adv. Mater. 2010, 22, 5297–5300. [Google Scholar] [CrossRef]
  21. Wu, C.S.; Bronder, T.; Poghossian, A.; Werner, C.F.; Schoening, M.J. Label-free detection of DNA using a light-addressable potentiometric sensor modified with a positively charged polyelectrolyte layer. Nanoscale 2015, 7, 6143–6150. [Google Scholar] [CrossRef] [PubMed]
  22. Branquinho, R.; Veigas, B.; Pinto, J.V.; Martins, R.; Fortunato, E.; Baptista, P.V. Real-time monitoring of PCR amplification of proto-oncogene c-MYC using a Ta2O5 electrolyte–insulator–semiconductor sensor. Biosens. Bioelectron. 2011, 28, 44–49. [Google Scholar] [CrossRef] [PubMed]
  23. Zhu, L.; Zhao, R.J.; Wang, K.G.; Xiang, H.B.; Shang, Z.M.; Sun, W. Electrochemical Behaviors of Methylene Blue on DNA Modified Electrode and Its Application to the Detection of PCR Product from NOS Sequence. Sensors 2008, 8, 5649–5660. [Google Scholar] [CrossRef] [PubMed]
  24. Wu, C.S.; Poghossian, A.; Bronder, T.; Schoening, M.J. Sensing of double-stranded DNA molecules by their intrinsic molecular charge using the light-addressable potentiometric sensor. Sens. Actuators B Chem. 2016, 229, 506–512. [Google Scholar] [CrossRef]
  25. Parthangal, P.M.; Cavicchi, R.E.; Zachariah, M.R. A universal approach to electrically connecting nanowire arrays using nanoparticles—Application to a novel gas sensor architecture. Nanotechnology 2006, 17, 3786–3790. [Google Scholar] [CrossRef]
  26. Guo, M.; Diao, P.; Cai, S. Hydrothermal growth of well-aligned ZnO nanorod arrays: Dependence of morphology and alignment ordering upon preparing conditions. J. Solid State Chem. 2005, 178, 1864–1873. [Google Scholar] [CrossRef]
  27. Zhang, Z.X.; Yuan, H.J.; Zhou, J.J.; Liu, D.F.; Luo, S.D.; Miao, Y.M.; Gao, Y.; Wang, J.X.; Liu, L.F.; Song, L.; et al. Growth Mechanism, Photoluminescence, and Field-Emission Properties of ZnO Nanoneedle Arrays. J. Phys. Chem. B 2006, 110, 8566–8569. [Google Scholar] [CrossRef]
  28. Ahsanulhaq, Q.; Umar, A.; Hahn, Y.B. Growth of aligned ZnO nanorods and nanopencils on ZnO/Si in aqueous solution: Growth mechanism and structural and optical properties. Nanotechnology 2007, 18, 115603. [Google Scholar] [CrossRef]
  29. Zong, X.L.; Wu, C.S.; Wu, X.L.; Lu, Y.F.; Wang, P. A non-labeled DNA biosensor based on LAPS modified with TiO2 thin film. J. Zhejiang Univ. Sci. B 2009, 10, 860–866. [Google Scholar] [CrossRef]
Sensors 19 05473 g001 550
Figure 1. Schematic diagram of the light-addressable potentiometric sensor (LAPS) measurement setup.
Figure 1. Schematic diagram of the light-addressable potentiometric sensor (LAPS) measurement setup.
Sensors 19 05473 g001
Sensors 19 05473 g002 550
Figure 2. SEM images of ZnO nanorod arrays (NRAs) deposited on the LAPS surface (a) before and (b) after silanization with 3-aminopropyltriethoxysilane (APTES).
Figure 2. SEM images of ZnO nanorod arrays (NRAs) deposited on the LAPS surface (a) before and (b) after silanization with 3-aminopropyltriethoxysilane (APTES).
Sensors 19 05473 g002
Sensors 19 05473 g003 550
Figure 3. (a) I–V curves recorded from LAPS chip with ZnO NRAs after silanization, probe ssDNA immobilization, and bovine serum albumin (BSA) blocking. (b) Shifts of I–V curves induced by probe ssDNA immobilization and BSA blocking on the surface of LAPS chips with and without ZnO NRAs. All the data are represented by the mean ± standard error of the mean (SEM). ** p < 0.01, Student’s t-test. The mean and SEM of three experiments are shown.
Figure 3. (a) I–V curves recorded from LAPS chip with ZnO NRAs after silanization, probe ssDNA immobilization, and bovine serum albumin (BSA) blocking. (b) Shifts of I–V curves induced by probe ssDNA immobilization and BSA blocking on the surface of LAPS chips with and without ZnO NRAs. All the data are represented by the mean ± standard error of the mean (SEM). ** p < 0.01, Student’s t-test. The mean and SEM of three experiments are shown.
Sensors 19 05473 g003
Sensors 19 05473 g004 550
Figure 4. (a) Shifts of I–V curves recorded from the LAPS chip with ZnO NRAs in response to asymmetric polymerase chain reaction (PCR) products from different concentrations of E. coli O157:H7. (b) Statistical results of potential shifts of I–V curves recorded from LAPS chips with and without ZnO NRAs, induced by target ssDNA amplified from different concentrations of E. coli O157:H7. All the data are represented by the mean ± standard error of the mean (SEM). The mean and SEM of six experiments are shown.
Figure 4. (a) Shifts of I–V curves recorded from the LAPS chip with ZnO NRAs in response to asymmetric polymerase chain reaction (PCR) products from different concentrations of E. coli O157:H7. (b) Statistical results of potential shifts of I–V curves recorded from LAPS chips with and without ZnO NRAs, induced by target ssDNA amplified from different concentrations of E. coli O157:H7. All the data are represented by the mean ± standard error of the mean (SEM). The mean and SEM of six experiments are shown.
Sensors 19 05473 g004

Share and Cite

MDPI and ACS Style

Tian, Y.; Liang, T.; Zhu, P.; Chen, Y.; Chen, W.; Du, L.; Wu, C.; Wang, P. Label-Free Detection of E. coli O157:H7 DNA Using Light-Addressable Potentiometric Sensors with Highly Oriented ZnO Nanorod Arrays. Sensors 2019, 19, 5473. https://doi.org/10.3390/s19245473

AMA Style

Tian Y, Liang T, Zhu P, Chen Y, Chen W, Du L, Wu C, Wang P. Label-Free Detection of E. coli O157:H7 DNA Using Light-Addressable Potentiometric Sensors with Highly Oriented ZnO Nanorod Arrays. Sensors. 2019; 19(24):5473. https://doi.org/10.3390/s19245473

Chicago/Turabian Style

Tian, Yulan, Tao Liang, Ping Zhu, Yating Chen, Wei Chen, Liping Du, Chunsheng Wu, and Ping Wang. 2019. "Label-Free Detection of E. coli O157:H7 DNA Using Light-Addressable Potentiometric Sensors with Highly Oriented ZnO Nanorod Arrays" Sensors 19, no. 24: 5473. https://doi.org/10.3390/s19245473

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop