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Volume 20
Issue 12
10.3390/s20123567
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Letter

Detecting Collagen Molecules at Picogram Level through Electric Field-Induced Accumulation

by
Romina Rega
1,*,
Martina Mugnano
1,
Emilia Oleandro
1,2,
Volodymyr Tkachenko
1,
Danila del Giudice
1,2,
Gianluca Bagnato
3,
Pietro Ferraro
1,
Simonetta Grilli
1 and
Sebastiano Gangemi
4
1
Department of Physical Science and Technology of Matter, Institute of Applied Sciences and Intelligent Systems (ISASI), National Research Council of Italy (CNR), 80078 Pozzuoli (NA), Italy
2
Department of Mathematics and Physics, University of Campania, 81100 Caserta, Italy
3
Division of Pneumology, Papardo Hospital, Contrada Papardo, 98122 Messina, Italy
4
School and Operative Unit of Allergy and Clinical Immunology, Department of Clinical and Experimental Medicine, University of Messina, 98122 Messina, Italy
*
Author to whom correspondence should be addressed.
Sensors 2020, 20(12), 3567; https://doi.org/10.3390/s20123567
Submission received: 28 May 2020 / Revised: 19 June 2020 / Accepted: 22 June 2020 / Published: 24 June 2020
(This article belongs to the Section Biosensors)
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Abstract

The demand for sensors capable of measuring low-abundant collagen in human fluids has highly increased in recent years. Indeed, collagen is expected to be a biomarker for chronic diseases and could monitor their progression. Here we show detection of highly diluted samples of collagen at picogram level thanks to an innovative pyro-electrohydrodynamic jet (p-jet) system. Through the intense electric fields generated by the pyroelectric effect in a ferroelectric crystal, the collagen solution was concentrated on a small area of a slide that was appropriately functionalized to bind proteins. The collagen molecules were labeled by an appropriate fluorophore to show how the number of tiny droplets influences the limit of detection of the technique. The results show that the p-jet is extremely promising for overcoming the current detection limits of collagen-based products in human fluids, performing 10 times better than the enzyme-linked immunosorbent assay (ELISA) and thus paving the way for the early diagnosis of related chronic diseases.
Keywords: collagen; lithium niobate; pyroelectric effect; pyro-electrohydrodynamic jet collagen; lithium niobate; pyroelectric effect; pyro-electrohydrodynamic jet

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MDPI and ACS Style

Rega, R.; Mugnano, M.; Oleandro, E.; Tkachenko, V.; del Giudice, D.; Bagnato, G.; Ferraro, P.; Grilli, S.; Gangemi, S. Detecting Collagen Molecules at Picogram Level through Electric Field-Induced Accumulation. Sensors 2020, 20, 3567. https://doi.org/10.3390/s20123567

AMA Style

Rega R, Mugnano M, Oleandro E, Tkachenko V, del Giudice D, Bagnato G, Ferraro P, Grilli S, Gangemi S. Detecting Collagen Molecules at Picogram Level through Electric Field-Induced Accumulation. Sensors. 2020; 20(12):3567. https://doi.org/10.3390/s20123567

Chicago/Turabian Style

Rega, Romina, Martina Mugnano, Emilia Oleandro, Volodymyr Tkachenko, Danila del Giudice, Gianluca Bagnato, Pietro Ferraro, Simonetta Grilli, and Sebastiano Gangemi. 2020. "Detecting Collagen Molecules at Picogram Level through Electric Field-Induced Accumulation" Sensors 20, no. 12: 3567. https://doi.org/10.3390/s20123567

APA Style

Rega, R., Mugnano, M., Oleandro, E., Tkachenko, V., del Giudice, D., Bagnato, G., Ferraro, P., Grilli, S., & Gangemi, S. (2020). Detecting Collagen Molecules at Picogram Level through Electric Field-Induced Accumulation. Sensors, 20(12), 3567. https://doi.org/10.3390/s20123567

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