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Sensors
Volume 22
Issue 5
10.3390/s22051842
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Communication

Use of a Rotating Square Spatial-Frequency Filter to Map the Optical Path Length Variation in Microscopic Biological Samples

by
Ignacio Iglesias
Departamento de Física, CEIR Campus Mare Nostrum (CMN), Universidad de Murcia, 30100 Murcia, Spain
Sensors 2022, 22(5), 1842; https://doi.org/10.3390/s22051842
Submission received: 30 November 2021 / Revised: 17 February 2022 / Accepted: 23 February 2022 / Published: 25 February 2022
(This article belongs to the Special Issue Three-Dimensional (3D) Biophotonics Sensing and Reconstruction towards Biomedical Research)
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Abstract

Gradient images can be obtained using a rotating square mask to filter the angular spectra of the wavefront generated by a complex transmittance object. This method can be applied to measure the three-dimensional structure of microscopic biological samples through the relationship of the phase with the optical path length. This work describes the implementation of a system using an inverted optical microscope and shows the experimental results of phase maps generated by boar sperm cells.
Keywords: microscopy; quantitative microscopy; wavefront sensing microscopy; quantitative microscopy; wavefront sensing

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MDPI and ACS Style

Iglesias, I. Use of a Rotating Square Spatial-Frequency Filter to Map the Optical Path Length Variation in Microscopic Biological Samples. Sensors 2022, 22, 1842. https://doi.org/10.3390/s22051842

AMA Style

Iglesias I. Use of a Rotating Square Spatial-Frequency Filter to Map the Optical Path Length Variation in Microscopic Biological Samples. Sensors. 2022; 22(5):1842. https://doi.org/10.3390/s22051842

Chicago/Turabian Style

Iglesias, Ignacio. 2022. "Use of a Rotating Square Spatial-Frequency Filter to Map the Optical Path Length Variation in Microscopic Biological Samples" Sensors 22, no. 5: 1842. https://doi.org/10.3390/s22051842

APA Style

Iglesias, I. (2022). Use of a Rotating Square Spatial-Frequency Filter to Map the Optical Path Length Variation in Microscopic Biological Samples. Sensors, 22(5), 1842. https://doi.org/10.3390/s22051842

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