Microbial Acoustical Analyzer for Antibiotic Indication
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacteria and Culture Conditions
2.2. Light Phase Contrast Microscopy
2.3. Antibiotics
2.4. Determination of the Viability of the Bacteria
2.5. Preparation of Cells before Carrying Out the Measurements
2.6. Description of the Analyzer and Technique of Experiments
3. Results and Discussion
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
- Oong, G.C.; Tadi, P. Chloramphenicol. In StatPearls; StatPearls Publishing: Treasure Island, FL, USA, 2022. Available online: https://www.ncbi.nlm.nih.gov/books/NBK555966/ (accessed on 25 February 2022).
- Shukla, P.; Bansode, F.W.; Singh, R.K. Chloramphenicol Toxicity: A Review. J. Med. Med. Sci. 2011, 2, 1313–1316. [Google Scholar]
- European Medicines Agency; European Surveillance of Veterinary Antimicrobial Consumption. Sales of Veterinary Antimicrobial Agents in 31 European Countries in 2018. (EMA/24309/2020). 2020. Available online: https://www.ema.europa.eu/en/documents/report/sales-veterinary-antimicrobial-agents-31-european-countries-2018-trends-2010-2018-tenth-esvac-report_en.pdf (accessed on 25 February 2022).
- European Medicines Agency; European Surveillance of Veterinary Antimicrobial Consumption. Sales of Veterinary Antimicrobial Agents in 31 European Countries in 2019 and 2020. (EMA/58183/2021). 2021. Available online: https://www.ema.europa.eu/en/documents/report/sales-veterinary-antimicrobial-agents-31-european-countries-2019-2020-trends-2010-2020-eleventh_en.pdf (accessed on 25 February 2022).
- Abdollahi, M.; Mostafalou, S. Chloramphenicol. In Encyclopedia of Toxicology, 3rd ed.; Wexler, P., Ed.; Academic Press: Cambridge, MA, USA, 2014; pp. 837–840. ISBN 9780123864550. [Google Scholar] [CrossRef]
- Wiest, D.B.; Cochran, J.B.; Tecklenburg, F.W. Chloramphenicol toxicity revisited: A 12-year-old patient with a brain abscess. J. Pediatr. Pharmacol. Ther. 2012, 17, 182–188. [Google Scholar] [CrossRef] [PubMed]
- Scholar, E. Chloramphenicol. In xPharm: The Comprehensive Pharmacology Reference; Enna, S.J., Bylund, D.B., Eds.; Elsevier: Amsterdam, The Netherlands, 2007; pp. 1–7. ISBN 9780080552323. [Google Scholar] [CrossRef]
- Pengov, A.; Flajs, V.C.; Zadnik, T.; Marinšek, J.; Pogačnik, M. Distribution of chloramphenicol residues in lactating cows following an external application. Anal. Chim. Acta. 2005, 529, 347–351. [Google Scholar] [CrossRef]
- Hanekamp, J.C.; Bast, A. Antibiotics exposure and health risks: Chloramphenicol. Environ. Toxicol. Pharmacol. 2015, 39, 213–220. [Google Scholar] [CrossRef]
- Li, J.; Shao, B.; Shen, J.; Wang, S.; Wu, Y. Occurrence of chloramphenicol-resistance genes as environmental pollutants from swine feedlots. Environ. Sci. Technol. 2013, 47, 2892–2897. [Google Scholar] [CrossRef]
- Tian, W.; Gao, L.; Zhao, Y.; Peng, W.; Chen, Z. Simultaneous determination of metronidazole, chloramphenicol and 10 sulfonamide residues in honey by LC–MS/MS. Anal. Methods 2013, 5, 1283–1288. [Google Scholar] [CrossRef]
- Vosough, M.; Mashhadiabbas, E.H. Fast HPLC-DAD quantification procedure for selected sulfonamids, metronidazole and chloramphenicol in wastewaters using second-order calibration based on MCR-ALS. Talanta 2013, 113, 68–75. [Google Scholar] [CrossRef]
- Liu, T.; Xie, J.; Zhao, J.; Song, G.; Hu, Y. Magnetic Chitosan Nanocomposite Used as Cleanup Material to Detect Chloramphenicol in Milk by GC-MS. Food Anal. Methods 2014, 7, 814–819. [Google Scholar] [CrossRef]
- Yan, W.J.; Yang, L.P.; Zhuang, H.; Wu, H.Z.; Zhang, J.H. Engineered “hot” core-shell nanostructures for patterned detection of chloramphenicol. Biosens. Bioelectron. 2015, 78, 67–72. [Google Scholar] [CrossRef]
- Moudgil, P.; Bedi, J.S.; Aulakh, R.S.; Gill, J.P.S.; Kumar, A. Validation of HPLC Multi-residue Method for Determination of fluoroquinolones, tetracycline, sulphonamides and chloramphenicol residues in bovine milk. Food Anal. Methods 2019, 12, 338–346. [Google Scholar] [CrossRef]
- Chen, H.; Chen, H.; Ying, J.; Huang, J.; Liao, L. Dispersive liquid–liquid microextraction followed by high-performance liquid chromatography as an efficient and sensitive technique for simultaneous determination of chloramphenicol and thiamphenicol in honey. Anal. Chim. Acta. 2009, 632, 80–85. [Google Scholar] [CrossRef] [PubMed]
- Xu, C.; Peng, C.; Hao, K.; Jin, Z.; Wang, W. Chemiluminescence enzyme immunoassay (CLEIA) for the determination of chloramphenicol residues in aquatic tissues. Luminescence 2006, 21, 126–128. [Google Scholar]
- Ding, Y.; Zhang, X.; Yin, H.; Meng, Q.; Zhao, Y.; Liu, L.; Wu, Z.; Xu, H. Quantitative and Sensitive Detection of Chloramphenicol by Surface-Enhanced Raman Scattering. Sensors 2017, 17, 2962. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Dong, X.; Yan, X.; Li, M.; Liu, H.; Li, J.; Wang, L.; Wang, K.; Lu, X.; Wang, S.; He, B. Ultrasensitive detection of chloramphenicol using electrochemical aptamer sensor: A mini review. Electrochem. Commun. 2020, 120, 106835. [Google Scholar] [CrossRef]
- Tao, X.; He, F.; Liu, X.; Zhang, F.; Wang, X.; Peng, Y.; Liu, J. Detection of chloramphenicol with an aptamer-based colorimetric assay: Critical evaluation of specific and unspecific binding of analyte molecules. Microchim. Acta 2020, 187, 668. [Google Scholar] [CrossRef]
- Hamidi-Asl, G.E.; Dardenneб, F.; Blust, R.; Wael, K.D. Electrochemical Aptasensor for Chloramphenicol Detection Based on Aptamer Incorporated Gelatine. Sensors 2015, 15, 7605–7618. [Google Scholar] [CrossRef] [Green Version]
- Neethu, S.; Yu, W.-C.; Deepak, B. Electrochemical detection of an antibiotic drug chloramphenicol based on a graphene oxide/hierarchical zinc oxide nanocomposite. Inorg. Chem. Front. 2019, 6, 82–93. [Google Scholar] [CrossRef]
- Park, I.S.; Kim, D.K.; Adanyi, N.; Varadi, M.; Kim, N. Development of a direct-binding chloramphenicol sensor based on thiol or sulfide mediated self-assembled antibody monolayers. Biosens. Bioelectron. 2004, 19, 667–674. [Google Scholar] [CrossRef]
- Kim, D.-M.; Rahman, M.A.; Do, M.H.; Ban, C.; Shim, Y.-B. An amperometric chloramphenicol immunosensor based on cadmium sulfide nanoparticles modified-dendrimer bonded conducting polymer. Biosens. Bioelectron. 2010, 25, 1781–1788. [Google Scholar] [CrossRef]
- Zhang, N.; Xiao, F.; Bai, J.; Lai, Y.; Hou, J.; Xian, Y.; Jin, L. Label-free immunoassay for chloramphenicol based on hollow gold nanospheres/chitosan composite. Talanta 2011, 87, 100–105. [Google Scholar] [CrossRef]
- Karaseva, N.A.; Ermolaeva, T.N. A piezoelectric immunosensor for chloramphenicol detection in food. Talanta 2012, 93, 44–48. [Google Scholar] [CrossRef] [PubMed]
- Levi, R.; McNiven, S.; Piletsky, S.A.; Cheong, S.H.; Yano, K.; Karube, I. Optical detection of chloramphenicol using molecularly imprinted polymers. Anal. Chem. 1997, 69, 2017–2021. [Google Scholar] [CrossRef] [PubMed]
- Sai, N.; Wu, Y.; Sun, Z.; Yu, G.; Huang, G. A novel photonic sensor for the detection of chloramphenicol. Arab. J. Chem. 2019, 12, 4398–4406. [Google Scholar] [CrossRef] [Green Version]
- Zhou, C.; Wang, T.; Liu, J.; Guo, C.; Peng, Y.; Bai, J.; Liu, M.; Dong, J.; Gao, N.; Ning, B.A.; et al. Molecularly imprinted photonic polymer as an optical sensor to detect chloramphenicol. Analyst 2012, 137, 4469–4474. [Google Scholar] [CrossRef]
- Schirmer, C.; Meisel, H. Synthesis of a molecularly imprinted polymer for the selective solid-phase extraction of chloramphenicol from honey. J. Chromatogr. A 2006, 1132, 325–328. [Google Scholar] [CrossRef]
- Shi, X.; Wu, A.; Zheng, S.; Li, R.; Zhang, D. Molecularly imprinted polymer microspheres for solid-phase extraction of chloramphenicol residues in foods. J. Chromatogr. B 2007, 850, 24–30. [Google Scholar] [CrossRef]
- Gao, F.; Feng, S.; Chen, Z.; Li-Chan, E.C.Y.; Grant, E.; Lu, X. Detection and quantification of chloramphenicol in milk and honey using molecularly imprinted polymers: Canadian penny-based SERS nano-biosensor. J. Food Sci. 2014, 79, N2542–N2549. [Google Scholar] [CrossRef]
- Zhao, H.; Chen, Y.; Tian, J.; Yu, H.; Quan, X. Selectively electrochemical determination of chloramphenicol in aqueous solution using molecularly imprinted polymer-carbon nanotubes-gold nanoparticles modified electrode. J. Electrochem. Soc. 2012, 159, J231–J236. [Google Scholar] [CrossRef]
- Nourmohammadi, E.; Hosseinkhani, S.; Nedaeinia, R.; Khoshdel Sarkarizi, H.; Nedaeinia, M.; Ranjbar, M.; Ebrahimi, N.; Farjami, Z.; Nourmohammadi, M.; Mahmoudi, A.; et al. Construction of a sensitive and specifc lead biosensor using a genetically engineered bacterial system with a luciferase gene reporter controlled by pbr and cadA promoters. BioMed. Eng. 2020, 19, 79. [Google Scholar] [CrossRef]
- Shin, H.J. Genetically engineered microbial biosensors for in situ monitoring of environmental pollution. Appl. Microbiol. Biotechnol. 2011, 89, 867–877. [Google Scholar] [CrossRef]
- Hillger, J.M.; Schoop, J.; Boomsma, D.I.; Slagboom, P.E.; Ijzerman, A.P.; Heitman, L.H. Whole-cell biosensor for label-free detection of GPCR-mediated drug responses in personal cell lines. Biosens. Bioelectron. 2015, 74, 233–242. [Google Scholar] [CrossRef] [PubMed]
- Magrisso, S.; Erel, Y.; Belkin, S. Microbial reporters of metal bioavailability. Microb. Biotechnol. 2008, 1, 320–330. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- He, W.; Yuan, S.; Zhong, W.H.; Siddikee, M.A.; Dai, C.C. Application of genetically engineered microbial whole-cell biosensors for combined chemosensing. Appl. Microbiol. Biotechnol. 2016, 100, 1109–1119. [Google Scholar] [CrossRef]
- Guliy, O.I.; Zaitsev, B.D.; Semyonov, A.P.; Alsowaidi, A.K.M.; Teplykh, A.A.; Karavaeva, O.A.; Borodina, I.A. Microbial acoustic sensor test-system based on a piezoelectric resonator with a lateral electric field for kanamycin detection in liquid. Ultrasonics 2022, 120, 106651. [Google Scholar] [CrossRef]
- Johnson, W.L.; France, D.C.; Rentz, N.S.; Cordell, W.T.; Walls, F.L. Sensing bacterial vibrations and early response to antibiotics with phase noise of a resonant crystal. Sci. Rep. 2017, 7, 12138. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Phillips, I.; Acar, J.; Bergan, T.; Degener, J.; Baquero, F.; Forsgren, A.; Schito, G.-C.; Wiedemann, B. Methods for the determination of susceptibility of bacteria to antimicrobial agents. Terminology, EUCAST Definitive Document. Clin. Microbiol. Infect. 1998, 4, 291–296. [Google Scholar] [CrossRef] [Green Version]
- Zaitsev, B.D.; Kuznetsova, I.E.; Semyonov, A.P.; Vasil’ev, A.A. New method of parasitic mode supression in lateral-field-excited piezoelectric resonator. Tech. Phys. Lett. 2011, 37, 503–506. [Google Scholar] [CrossRef]
- Carone, B.R.; Xu, T.; Murphy, K.C.; Marinus, M.G. High incidence of multiple antibiotic resistant cells in cultures of in enterohemorrhagic Escherichia coli O157:H7. Mutat. Res. 2014, 759, 1–8. [Google Scholar] [CrossRef] [Green Version]
- Zaitsev, B.D.; Shikhabudinov, A.M.; Teplykh, A.A.; Kuznetsova, I.E. Liquid sensor based on a piezoelectric lateral electric field-excited resonator. Ultrasonics 2015, 63, 179–183. [Google Scholar] [CrossRef]
- EFSA CONTAM Panel (EFSA Panel on Contaminants in the Food Chain). Scientific Opinion on Chloramphenicol in food and feed. EFSA J. 2014, 12, 3907. [Google Scholar] [CrossRef]
|ΔZ|, kHz | |ΔZav|, kHz | n, µg/mL | nav, µg/mL | Δnav, µg/mL | Sq, µg/mL | Res1 | Res5 |
---|---|---|---|---|---|---|---|
0.764 | 0.764 ± 1.6% | 4.5 | 4.534 | 0.034 | 0.17 | 4.534 ± 0.17 4.534 ± 3.76% | 4.534 ± 0.08 4.52 ± 1.7% |
0.779 | 4.79 | −0.256 | |||||
0.749 | 4.35 | 0.184 | |||||
0.771 | 4.6 | −0.066 | |||||
0.756 | 4.43 | 0.104 | |||||
1.028 | 1.022 ± 1.75% | 7 | 7.02 | 0.02 | 0.135 | 7.02 ± 0.135 7.02 ± 2% | 7.02 ± 0.06 7.02 ± 0.91% |
1.05 | 7.2 | −0.18 | |||||
1.009 | 6.85 | 0.17 | |||||
1.039 | 7.1 | −0.08 | |||||
1.019 | 6.95 | 0.07 | |||||
1.241 | 1.24 ± 1.5% | 9.05 | 9.01 | −0.04 | 0.192 | 9.01 ± 0.192 9.01 ± 2.1% | 9.01 ± 0.09 9.01 ± 0.95% |
1.264 | 9.25 | −0.24 | |||||
1.215 | 8.75 | 0.26 | |||||
1.252 | 9.1 | −0.09 | |||||
1.227 | 8.9 | 0.11 |
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Zaitsev, B.; Borodina, I.; Alsowaidi, A.; Karavaeva, O.; Teplykh, A.; Guliy, O. Microbial Acoustical Analyzer for Antibiotic Indication. Sensors 2022, 22, 2937. https://doi.org/10.3390/s22082937
Zaitsev B, Borodina I, Alsowaidi A, Karavaeva O, Teplykh A, Guliy O. Microbial Acoustical Analyzer for Antibiotic Indication. Sensors. 2022; 22(8):2937. https://doi.org/10.3390/s22082937
Chicago/Turabian StyleZaitsev, Boris, Irina Borodina, Ali Alsowaidi, Olga Karavaeva, Andrey Teplykh, and Olga Guliy. 2022. "Microbial Acoustical Analyzer for Antibiotic Indication" Sensors 22, no. 8: 2937. https://doi.org/10.3390/s22082937