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Volume 23
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10.3390/s23063209
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Article

Glucose Biosensor Based on Glucose Oxidase Immobilized on BSA Cross-Linked Nanocomposite Modified Glassy Carbon Electrode

by
Yang-Yang Li
1,†,
Xin-Xin Ma
1,†,
Xin-Yan Song
1,
Lin-Lin Ma
1,
Yu-Ying Li
1,
Xin Meng
1,
Yu-Jie Chen
1,
Ke-Xin Xu
1,
Ali Akbar Moosavi-Movahedi
2,
Bao-Lin Xiao
1,* and
Jun Hong
1,*
1
School of Life Sciences, Henan University, Kaifeng 475000, China
2
Institute of Biochemistry and Biophysics, University of Tehran, Tehran 1417614418, Iran
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Sensors 2023, 23(6), 3209; https://doi.org/10.3390/s23063209
Submission received: 14 February 2023 / Revised: 11 March 2023 / Accepted: 16 March 2023 / Published: 17 March 2023
(This article belongs to the Section Biosensors)
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Abstract

Glucose sensors based blood glucose detection are of great significance for the diagnosis and treatment of diabetes because diabetes has aroused wide concern in the world. In this study, bovine serum albumin (BSA) was used to cross-link glucose oxidase (GOD) on a glassy carbon electrode (GCE) modified by a composite of hydroxy fullerene (HFs) and multi-walled carbon nanotubes (MWCNTs) and protected with a glutaraldehyde (GLA)/Nafion (NF) composite membrane to prepare a novel glucose biosensor. The modified materials were analyzed by UV-visible spectroscopy (UV-vis), transmission electron microscopy (TEM), and cyclic voltammetry (CV). The prepared MWCNTs-HFs composite has excellent conductivity, the addition of BSA regulates MWCNTs-HFs hydrophobicity and biocompatibility, and better immobilizes GOD on MWCNTs-HFs. MWCNTs-BSA-HFs plays a synergistic role in the electrochemical response to glucose. The biosensor shows high sensitivity (167 μA·mM−1·cm−2), wide calibration range (0.01–3.5 mM), and low detection limit (17 μM). The apparent Michaelis–Menten constant Kmapp is 119 μM. Additionally, the proposed biosensor has good selectivity and excellent storage stability (120 days). The practicability of the biosensor was evaluated in real plasma samples, and the recovery rate was satisfactory.
Keywords: glucose oxidase; biosensor; hydroxyl fullerenes; multi-walled carbon nanotubes; bovine serum albumin glucose oxidase; biosensor; hydroxyl fullerenes; multi-walled carbon nanotubes; bovine serum albumin

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MDPI and ACS Style

Li, Y.-Y.; Ma, X.-X.; Song, X.-Y.; Ma, L.-L.; Li, Y.-Y.; Meng, X.; Chen, Y.-J.; Xu, K.-X.; Moosavi-Movahedi, A.A.; Xiao, B.-L.; et al. Glucose Biosensor Based on Glucose Oxidase Immobilized on BSA Cross-Linked Nanocomposite Modified Glassy Carbon Electrode. Sensors 2023, 23, 3209. https://doi.org/10.3390/s23063209

AMA Style

Li Y-Y, Ma X-X, Song X-Y, Ma L-L, Li Y-Y, Meng X, Chen Y-J, Xu K-X, Moosavi-Movahedi AA, Xiao B-L, et al. Glucose Biosensor Based on Glucose Oxidase Immobilized on BSA Cross-Linked Nanocomposite Modified Glassy Carbon Electrode. Sensors. 2023; 23(6):3209. https://doi.org/10.3390/s23063209

Chicago/Turabian Style

Li, Yang-Yang, Xin-Xin Ma, Xin-Yan Song, Lin-Lin Ma, Yu-Ying Li, Xin Meng, Yu-Jie Chen, Ke-Xin Xu, Ali Akbar Moosavi-Movahedi, Bao-Lin Xiao, and et al. 2023. "Glucose Biosensor Based on Glucose Oxidase Immobilized on BSA Cross-Linked Nanocomposite Modified Glassy Carbon Electrode" Sensors 23, no. 6: 3209. https://doi.org/10.3390/s23063209

APA Style

Li, Y.-Y., Ma, X.-X., Song, X.-Y., Ma, L.-L., Li, Y.-Y., Meng, X., Chen, Y.-J., Xu, K.-X., Moosavi-Movahedi, A. A., Xiao, B.-L., & Hong, J. (2023). Glucose Biosensor Based on Glucose Oxidase Immobilized on BSA Cross-Linked Nanocomposite Modified Glassy Carbon Electrode. Sensors, 23(6), 3209. https://doi.org/10.3390/s23063209

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