2. DNA-electrochemical biosensors
DNA biosensors are integrated receptor-transducer devices that use DNA as biomolecular recognition element to measure specific binding processes with DNA, usually by electrical, thermal, or optical signal transduction [
1].
Compared with other transducers, electrochemical ones received particular interest due to a rapid detection and great sensitivity. Combining the characteristics of DNA probes with the capacity of direct and label-free electrochemical detection represents an attractive solution in many different fields of application, such as rapid monitoring of pollutant agents or metals in the environment, investigation and evaluation of DNA-drug interaction mechanisms, detection of DNA base damage in clinical diagnosis, or detection of specific DNA sequences in human, viral, and bacterial nucleic acids [
2-
8]. Amongst the electrochemical transducers, carbon electrodes such as glassy carbon, carbon fibre, graphite, or carbon black exhibit several unique properties. The wide electrochemical potential window in the positive direction allows sensitive electrochemical detection of oxidative damage caused to DNA by monitoring the appearance of oxidation peaks of DNA bases.
To design efficient DNA-electrochemical biosensors, it is essential to know the structure and to understand the electrochemical characteristics of DNA molecules.
DNA consists of two antiparallel polynucleotide chains formed by monomeric nucleotide units. Each nucleotide is formed by three types of chemical components: a phosphate group, a sugar called deoxyribose, and four different nitrogen bases. The phosphate-deoxyribose sugar polymer represents the DNA backbone. The cellular genetic information is coded by the purine bases, adenine (A) and guanine (G), and the pyrimidine bases, cytosine (C) and thymine (T), as a function of their consecutive order in the chain. The two strands of nucleotides are twisted into a double helix, held together by hydrogen bonds between the A·T and G·C bases of each strand.
Electrochemical oxidation of DNA at a glassy carbon electrode [
2,
3] using differential pulse voltammetry shows two current peaks due to the oxidation of guanine and adenine residues,
Fig. 1. The electrochemical behaviour obtained with double stranded DNA (dsDNA) and single stranded DNA (ssDNA) illustrates the greater difficulty for the transfer of electrons from the inside of the double-stranded rigid form of DNA to the electrode surface, than from the flexible single stranded form of DNA where the bases are in close proximity to the electrode surface. The roughness of the solid electrode surface means that dsDNA has some difficulty in following the surface contours whereas unwound ssDNA molecules fit more easily into the grooves of the surface, owing to their greater flexibility.
However, it was a challenge to increase the sensitivity and to allow simultaneous electrochemical detection of all purine and pyrimidine DNA bases in natural samples at physiological pH, and to extend
the DNA voltammetric studies to nucleotides so as to make possible the simultaneous detection of all bases in single-stranded DNA (ssDNA) without the necessity of a previous hydrolysis step.
Mechanistic studies on the electrochemical behaviour of purine and pyrimidine derivatives have been performed during the past four decades. Electrochemical oxidation on carbon electrodes [
8-
12] showed that all bases - guanine, adenine, thymine and cytosine - could be oxidized following a pH dependent mechanism. For the first time equimolar mixtures of all DNA bases, nucleosides, and nucleotides were quantified by differential pulse voltammetry,
Fig. 2 [
12].
Electrochemical preconditioning of the glassy carbon electrode (GCE) enabled the achievement of a better peak separation and an enhancement of the oxidation peak currents for all four DNA bases in pH 7.4 phosphate buffer supporting electrolyte, close to physiological pH. Detection limits in the nano-and micromolar ranges were obtained for purine and pyrimidine bases, respectively, together in solution. The results also showed for the first time that the pyrimidine nucleosides and nucleotides are electroactive on glassy carbon electrodes and that, in addition to the easy detection of the purines, it was also possible to detect simultaneously the oxidation of pyrimidine residues in ssDNA.
When natural or synthetic DNA molecules interact with electrode surfaces, adsorption occurs. The adsorption of dsDNA is quasi-reversible, and its structure undergoes conformational modifications during immobilization on the electrodes. Observing DNA adsorption was the first step in developing DNA-modified electrodes, and thence DNA-electrochemical biosensors. DNA immobilization and the stabilization procedure of the DNA probe on the electrode surface represent key aspects in the manufacture of a DNA-electrochemical biosensor, influencing the characteristics of the DNA probe, the sensor response and its performance [
13]. Different structural conformations adopted by DNA can lead to different interactions with small molecules and to changes in the accessibility of drugs to the DNA grooves. A good understanding of all these factors is essential in order to design surfaces exhibiting good biological activity and selectivity.
Different immobilization procedures of monolayer and multilayer DNA films on highly oriented pyrolytic graphite (HOPG) electrodes have been characterized by Magnetic AC Mode Atomic Force Microscopy (MAC AFM) [
14-
17]. HOPG provides a particularly smooth electrode surface,
Fig. 3A, which enables the detection of topographical modifications when DNA molecules are adsorbed. Direct observation of the electrode surface demonstrated that the characteristics of the DNA film and the optimal electrochemical response of the DNA-biosensor depend on pH, buffer composition, ionic strength, immobilization procedure, adsorption time, applied potential, type and concentration of DNA.
At small DNA concentrations, thin film dsDNA and ssDNA layers are formed, that appear as large-scale network structures uniformly covering the HOPG surface,
Figs. 3B and
4. Large pores in the film leave parts of the electrode uncovered. Thicker films prepared by different adsorption immobilization procedures show uniform and complete coverage of the electrode, with uniformly distributed peaks and valleys,
Fig. 3C and D. DNA films grown in more acid buffer solutions always present greater DNA surface coverage, due to overlaying and superposition of DNA molecules, with respect to films formed in neutral buffer solutions,
Fig. 4. The DNA-electrode surface interactions are stronger and the DNA films more stable when a potential is applied during adsorption. Therefore the DNA immobilization procedure must be a compromise between the degree of electrode coverage and strong adsorption due to the applied potential.
The dsDNA networks created during the formation of thin layers define different active surface areas on the DNA-electrochemical biosensor and form a biomaterial matrix to attach and study interaction with other molecules such as drugs. Due to the existence of pores in the DNA structure, some areas of the electrode surface are not covered by the molecular film and may act as a system of microelectrodes with nanometer or micrometer dimensions. These pores can cause misleading results when using a DNA electrochemical biosensor for detection of DNA-drug interactions. The fact that the electrode is not completely covered may allow the diffusion of drug molecules from bulk solution to the surface and their non-specific adsorption on the uncovered areas, leading to two contributions to the electrochemical signal, one from the simple adsorbed drug and the other due to damage of immobilized dsDNA, it not being easy to distinguish between the two signals. By using thick film DNA-biosensors, where the undesired binding of drug molecules to the electrode surface is impossible due to complete coverage of the electrode surface,
Fig. 3 C and D, the DNA-biosensor response is only determined by interaction of the compound with the dsDNA in the film. Knowledge of the morphology of adsorbed DNA, controlled by the appropriate choice of DNA solution concentration, pH and applied potential, helps in the improvement and development of specific DNA electrochemical biosensors.
3. DNA oxidative biomarker
Oxidative DNA damage caused by oxygen-free radicals leads to multiple modifications in DNA, including base-free sites and oxidized bases. The damage caused to DNA bases is potentially mutagenic [
18,
19]. The DNA oxidative damage can be enzymatically repaired.
The major product of DNA oxidative damage is 8-oxo-7,8-dihydroguanine (8-oxoGua) which is the oxidation product of guanine, the most easily oxidized base in DNA. This modified base is highly mutagenic [
20], that potentially mispairs with adenine during DNA replication, causing GC→TA transversion and loss of base-pairing specificity [
21]. Since it was first reported two decades ago, 8-oxoGua, in particular its deoxynucleoside, 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodGuo) has been the subject of intensive investigation and has become widely accepted as a biomarker of oxidative
DNA damage and cellular oxidative stress [
22,
23]. Also, 8-oxoG and 8-oxodGuo are major products of DNA oxidative damage, being excreted into urine.
At present, HPLC with electrochemical detection (HPLC-ECD) is the most commonly used technique to assess urinary 8-oxodGuo, but HPLC with tandem mass spectrometry (HPLC-MS/MS) is also used, showing high sensitivity and better specificity. However, HPLC-ECD is easier to use and less demanding of resources. There is a significant lack of HPLC-ECD based methods for 8-oxoGua detection in human urine, despite 8-oxoGua also being electrochemically detectable [
24] at a lower potential than 8-oxodGuo [
25]. A selective method based on HPLC-ECD was developed to enable simultaneous detection of 8-oxo-7,8-dihydroguanine and 8-oxo-7,8-dihydro-2′-deoxyguanosine, products of DNA oxidative damage, after uric acid (UA) elimination by uricase, consisting of HPLC isocratic elution with amperometric detection, enabling a detection limit for 8-oxoGua and 8-oxodGuo lower than 1 nM in standard mixtures [
26,
27]. Despite the complexity and inter-individual variability of urine samples, the method was successfully tested with urine samples from children with metabolic disorders and it was confirmed that at the applied working potential, hypoxanthine, present in urine, does not give any electrochemical signal and does not interfere at all in the 8-oxoGua signal.
The oxidation of the other DNA bases is much more difficult due to their high oxidation potentials [
8,
12]. Chemical modification of each of the DNA bases causes molecular disturbance to the genetic machinery that leads to cell malfunction. Oxidative damage to DNA by free radicals and exposure to ionizing radiation generates several products within the double helix besides 8-oxoGua, such as 2,8-dihydroxyadenine, 5-formyluracil, 5-hydroxycytosine, etc., which are mutagenic [
19].
Hence, there is great interest in the sensitive determination and full characterization of the mechanism involved in oxidative damage to DNA bases. Electrochemical methods are very promising for the study of DNA oxidative damage as well as in the investigation of the mechanisms of interactions of drugs with DNA. In particular, electrochemical DNA-biosensors have proved to be excellent tools for investigating the effect of various endogenous and exogenous sources of hazard to genomic material, allowing quick and low cost determination of DNA damage.
4. Electrochemical biosensors for detection of DNA damage
There are hundreds of compounds that bind and interact with DNA: toxic chemicals damage DNA and can cause human cancer. The factors that determine affinity and selectivity in binding molecules to DNA need to be explained, because a quantitative understanding of the reasons that determine selection of DNA reaction sites is useful in designing sequence-specific DNA binding molecules for application in chemotherapy and in explaining the mechanism of action of neoplasic drugs [
29].
Metal ions exist in body fluids and the nucleic acids and nucleotides occur as complexes coordinated [
30] with these ions. Some of these divalent ions are recognized for their carcinogenicity since they damage DNA and alter the fidelity of DNA synthesis [
31]. Although the possible relation between oxidative damage and metal concentrations is not completely clear, it is generally accepted that metals react with superoxide anion (O
2−•) and H
2O
2 to produce highly reactive species such as hydroxyl free radicals (OH•) and metal oxygen complexes in biological systems, resulting in metal-mediated oxidative DNA damage.
The cytostatic activity of various platinum drugs has shown that platinum coordination complexes cause irreversible inhibition of DNA synthesis due to covalent binding with DNA. This other causes the treatment to be accompanied by adverse reactions. The DNA-biosensor was successfully used for the electrochemical determination of carboplatin in serum samples of patients with ovarian cancer undergoing treatment [
32]. The mechanism of action of carboplatin with DNA clearly demonstrates that for low concentrations, carboplatin interacts preferentially with adenine rather than guanine groups and this can contribute to clarifying the mechanisms of interaction of platinum anti-cancer compounds with DNA.
Metals are considered to act not only as carcinogens but also to activate carcinogenic chemicals. A number of aromatic compounds induce oxidative DNA damage through metal catalyzed reactive oxygen species (ROS) generation. ROS produced in vivo react with DNA and its precursors modifying them, therefore, giving rise to the so-called oxidative stress. It is thought that the modification of DNA (DNA lesions) leads to the formation of incorrect base pairs, changes in the genetic information, which induces mutagenesis and carcinogenesis. Therefore, in a health preventing perspective, there is a deep interest in identifying free radical scavengers or anti-oxidants that inhibit oxidative DNA damage. Owing to their polyphenolic nature, flavonoids, compounds found in rich abundance in all land plants, often exhibit strong antioxidant properties [
33,
34,
35,
36,
37]. Initially, flavonoids were investigated as potential chemopreventive agents against certain carcinogens. Previous intake of a large quantity of flavonoid inhibited the incidence of ROS produced damages to DNA. In sharp contrast with their commonly accepted role, there is also considerable evidence that flavonoids themselves are mutagenic and have DNA damaging ability [
34,
35].
One striking example reported in the literature is that of quercetin, which in the presence of transition metals acts as a pro-oxidant and has mutagenic activity [
38,
39]. It was shown that some flavonoids are able to reduce transition metals, a process during which the highly oxidizing radical OH• is formed and could therefore damage DNA.
In an electrochemical study of the DNA-Cu(II)-quercetin interaction [
40], several situations were studied using a bare GCE in a solution containing dsDNA incubated with quercetin or quercetin-Cu(II) complex. Whereas a very weak interaction between quercetin and DNA was found to take place in solution, the addition of Cu(II) ions to the DNA-quercetin solution showed that a deep degradation of the DNA helix takes place. Using spectrophotometric techniques as a complement to electrochemical methods, it was observed that dsDNA damage occurred over time which suggests that the quercetin- Cu(II) complex intercalates with dsDNA and slowly interacts with it causing breaking of the hydrogen bonds.
Electrochemical voltammetric
in situ sensing of dsDNA oxidative damage caused by oxidized quercetin incorporated into dsDNA was possible using a DNA-electrochemical biosensor [
41]. The results indicate that quercetin binds to the dsDNA and can undergo oxidation at
Epa = + 0.32 V,
Fig. 5 . It is known that oxidation of quercetin is a complex process during which superoxide radicals are formed. These radicals readily attack the dsDNA disrupting the helix, causing contact between the electrode surface and the DNA nucleotides guanosine,
Epa = + 1.02 V, and adenosine,
Epa= +1.28 V, such that their oxidation peaks increase,
Fig. 5 . On the other hand, the radicals formed during the oxidation of quercetin incorporated into dsDNA lead to the formation of 8-oxoguanine, with peak at
Epa = + 0.45 V. In order to prove the involvement of the oxygen radicals in DNA damage during oxidation of intercalated quercetin, experiments were also carried out in solutions saturated with N
2,
Fig. 5 . The differential pulse voltammogram obtained showed only small guanosine and adenosine oxidation peaks, demonstrating that no DNA damage had occurred. Also, no additional peak, at
+ 0.450 V (8-oxoGua) was observed although a smaller quercetin oxidation peak 1 appeared. A model for this electrochemically observed in situ oxidative damage has been proposed and may be used to explain the oxidant-antioxidant effect of quercetin.
In a similar way, the detection of DNA damage involving strand breaks was observed using a hanging mercury drop electrode (HMDE) [
42]. Extensive cleavage of electrode-confined DNA by reactive oxygen species (ROS) was obtained in the absence of chemical reductants when redox cycling of metal (iron/DNA) complex was controlled. The cleavage agents were detected and the DNA cleavage was modulated, by generating the DNA-damaging species electrochemically.
The detection of chemicals that cause irreversible damage to DNA is also very important, and a short-time screening test for carcinogens based on ac-voltammetric measurements was developed in order to study in vitro damage to DNA caused by the action of alkylating mutagens [
43]. Nitroimidazoles are among the most important nitroheterocyclic drugs of interest in cancer chemotherapy. It was observed that adenine and guanine interact with intermediates generated during nitroimidazole reduction, causing irreversible damage to DNA and suggesting mutagenic properties of these compounds.
The mechanism of reduction of a group of nitroimidazoles was investigated using the DNA-biosensor [
44,
45,
46]. The analyte was pre-concentrated on the electrode surface containing DNA and either the reduction or the oxidation of the reduction products retained on the electrode surface was studied. It was possible to follow their reduction, the reversible oxidation of the hydroxylamine derivative formed (RNHOH) to the corresponding nitroso derivative (RNO), the condensation reaction between the hydroxylamine and nitroso derivatives to form the azoxycompound (RNO:NR) and interaction with DNA.
Conventional cancer chemotherapy is as toxic as it is beneficial. The DNA interacting drugs prevent cell growth, but not only cancer cell growth; the cytotoxic effect also blocks the growth of normal cells. The lack of selectivity of cancer drugs is one of the main problems in cancer chemotherapy [
47,
48]. The DNA-biosensors are an important tool for the investigation of the electrochemical and biological mechanism of drugs active against cancer [
4,
7,
28,
32,
44,
49].
Electrochemical voltammetric
in situ detection of dsDNA oxidative damage caused by reduced adriamycin [
7], an antibiotic of the family of anthracyclines, intercalated into DNA, was carried out using a DNA-biosensor. Oxidation and reduction of adriamycin molecules intercalated in dsDNA were investigated in order to understand the in vivo mechanism of action of DNA with this anti-neoplasic drug. The results showed that the interaction of adriamycin with dsDNA is potential-dependent. A mechanism for adriamycin reduction and oxidation
in situ when intercalated in dsDNA immobilized onto the glassy carbon electrode surface was proposed. This mechanism leads to the formation of the mutagenic 8-oxoGua, whose redox behaviour was studied.
Thiophene-S-oxides are a new group of compounds with biological activity against a number of cancer cells, only recently isolated and characterized in their pure form [
50]. The
in situ electrochemical detection of dsDNA damage caused by the reduced thiophene-S-oxide was detected using a glassy carbon electrode modified with a thiophene-S-oxide/dsDNA mixture. The results indicate that the reduced thiophene-S-oxide interacts with dsDNA, causing damage with possible strand breaks and that the thiophene-S-oxide adduct formed with dsDNA can still undergo reduction. The potential use of a compound/dsDNA film-modified glassy carbon electrode for understanding dsDNA interaction with molecules insoluble in water was confirmed.
The halogenated salicylanilides are a large group of compounds, which have been developed mainly due to their antiparasitic activity in animals. The investigation of the niclosamide-/DNA interaction [
51] using an electrochemical DNA-biosensor showed for the first time clear evidence of interaction with DNA and suggested that niclosamide toxicity can be caused by this interaction, after reductive activation.