Multi-instrumental Analysis of Tissues of Sunflower Plants Treated with Silver(I) Ions – Plants as Bioindicators of Environmental Pollution
Abstract
:1. Introduction
1.1 Silver ions and their effects on organisms
1.2 Bio-indicators
1.3 Silver ions analysis
2. Material and Methods
2.1 Chemicals and pH measurements
2.2 Plants, cultivation conditions and a sample preparation
2.3 Laser spectrometry
2.4 Automated spectrometric measurements
2.4.1 Urease activity determination – An indophenol assay (Berthelot method)
2.4.2 Protein determination – Biuret test
2.5 Anatomical analysis of plant samples
2.6 Statistical analysis
3. Results and Discussion
3.1 Physiological changes in sunflower plants exposed to silver(I) ions
3.2 Changes in the treated plants fresh weight
3.3 Anatomical changes of sunflower shoots (hypocotyls, stems) and roots
3.4 Spatial distribution of silver(I) ions in the plant tissues
3.5.1 The total protein content
3.5.2 Urease activity
3.6 The changes of urease activity in the presence of silver(I) ions
4. Conclusion
Acknowledgments
References
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Krizkova, S.; Ryant, P.; Krystofova, O.; Adam, V.; Galiova, M.; Beklova, M.; Babula, P.; Kaiser, J.; Novotny, K.; Novotny, J.; et al. Multi-instrumental Analysis of Tissues of Sunflower Plants Treated with Silver(I) Ions – Plants as Bioindicators of Environmental Pollution. Sensors 2008, 8, 445-463. https://doi.org/10.3390/s8010445
Krizkova S, Ryant P, Krystofova O, Adam V, Galiova M, Beklova M, Babula P, Kaiser J, Novotny K, Novotny J, et al. Multi-instrumental Analysis of Tissues of Sunflower Plants Treated with Silver(I) Ions – Plants as Bioindicators of Environmental Pollution. Sensors. 2008; 8(1):445-463. https://doi.org/10.3390/s8010445
Chicago/Turabian StyleKrizkova, Sona, Pavel Ryant, Olga Krystofova, Vojtech Adam, Michaela Galiova, Miroslava Beklova, Petr Babula, Jozef Kaiser, Karel Novotny, Jan Novotny, and et al. 2008. "Multi-instrumental Analysis of Tissues of Sunflower Plants Treated with Silver(I) Ions – Plants as Bioindicators of Environmental Pollution" Sensors 8, no. 1: 445-463. https://doi.org/10.3390/s8010445