Express Method for Isolation of Ready-to-Use 3D Chitin Scaffolds from Aplysina archeri (Aplysineidae: Verongiida) Demosponge

Klinger, Christine; Żółtowska-Aksamitowska, Sonia; Wysokowski, Marcin; Tsurkan, Mikhail V.; Galli, Roberta; Petrenko, Iaroslav; Machałowski, Tomasz; Ereskovsky, Alexander; Martinović, Rajko; Muzychka, Lyubov; Smolii, Oleg B.; Bechmann, Nicole; Ivanenko, Viatcheslav; Schupp, Peter J.; Jesionowski, Teofil; Giovine, Marco; Joseph, Yvonne; Bornstein, Stefan R.; Voronkina, Alona; Ehrlich, Hermann

doi:10.3390/md17020131

Open AccessArticle

Express Method for Isolation of Ready-to-Use 3D Chitin Scaffolds from Aplysina archeri (Aplysineidae: Verongiida) Demosponge

by

Christine Klinger

¹,

Sonia Żółtowska-Aksamitowska

^2,3,

Marcin Wysokowski

^2,3,*

,

Mikhail V. Tsurkan

⁴

,

Roberta Galli

⁵

,

Iaroslav Petrenko

³,

Tomasz Machałowski

²

,

Alexander Ereskovsky

^6,7

,

Rajko Martinović

⁸,

Lyubov Muzychka

⁹,

Oleg B. Smolii

⁹,

Nicole Bechmann

¹⁰

,

Viatcheslav Ivanenko

^11,12

,

Peter J. Schupp

¹³

,

Teofil Jesionowski

²

,

Marco Giovine

¹⁴,

Yvonne Joseph

³

,

Stefan R. Bornstein

^15,16,

Alona Voronkina

¹⁷

and

Hermann Ehrlich

^3,*

¹

Institute of Physical Chemistry, TU Bergakademie-Freiberg, Leipziger str. 29, 09559 Freiberg, Germany

²

Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, 61131 Poznan, Poland

³

Institute of Electronics and Sensor Materials, TU Bergakademie Freiberg, Gustav Zeuner Str. 3, 09599 Freiberg, Germany

⁴

Leibnitz Institute of Polymer Research Dresden, 01069 Dresden, Germany

⁵

Clinical Sensoring and Monitoring, Department of Anesthesiology and Intensive Care Medicine, Faculty of Medicine, Technische Universität Dresden, 01307 Dresden, Germany

⁶

Institut Méditerranéen de Biodiversité et d’Ecologie (IMBE), CNRS, IRD, Aix Marseille Université, Avignon Université, Station Marine d’Endoume, 13003 Marseille, France

⁷

Department of Embryology, Faculty of Biology, Saint-Petersburg State University, 19992 Saint-Petersburg, Russia

⁸

Institute of Marine Biology, University of Montenegro, 85330 Kotor, Montenegro

⁹

Institute of Bioorganic Chemistry and Petrochemistry, National Academy of Science of Ukraine, Murmanska Str., 1, 02094 Kyiv, Ukraine

¹⁰

Institute of Clinical Chemistry and Laboratory Medicine, University Hospital Carl Gustav Carus, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany

¹¹

Department of Invertebrate Zoology, Biological Faculty, Lomonosov Moscow State University, 119992 Moscow, Russia

¹²

Naturalis Biodiversity Center, 2332 Leiden, The Netherlands

¹³

Institute for Chemistry and Biology of the Marine Environment, University of Oldenburg, Carl-von-Ossietzky-Straße 9-11, 26111 Oldenburg, Germany

¹⁴

Department of Sciences of Earth, Environment and Life, University of Genoa, Corso Europa 26, 16132 Genova, Italy

¹⁵

Department of Internal Medicine III, University Hospital Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany

¹⁶

Diabetes and Nutritional Sciences Division, King’s College London, London WC2R 2LS, UK

¹⁷

National Pirogov Memorial Medical University, Vinnytsya, Department of Pharmacy, Pirogov str. 56, 21018, Vinnytsia, Ukraine

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Mar. Drugs 2019, 17(2), 131; https://doi.org/10.3390/md17020131

Submission received: 26 January 2019 / Revised: 16 February 2019 / Accepted: 19 February 2019 / Published: 22 February 2019

(This article belongs to the Special Issue Marine Chitin 2019)

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Abstract

Sponges are a valuable source of natural compounds and biomaterials for many biotechnological applications. Marine sponges belonging to the order Verongiida are known to contain both chitin and biologically active bromotyrosines. Aplysina archeri (Aplysineidae: Verongiida) is well known to contain bromotyrosines with relevant bioactivity against human and animal diseases. The aim of this study was to develop an express method for the production of naturally prefabricated 3D chitin and bromotyrosine-containing extracts simultaneously. This new method is based on microwave irradiation (MWI) together with stepwise treatment using 1% sodium hydroxide, 20% acetic acid, and 30% hydrogen peroxide. This approach, which takes up to 1 h, made it possible to isolate chitin from the tube-like skeleton of A. archeri and to demonstrate the presence of this biopolymer in this sponge for the first time. Additionally, this procedure does not deacetylate chitin to chitosan and enables the recovery of ready-to-use 3D chitin scaffolds without destruction of the unique tube-like fibrous interconnected structure of the isolated biomaterial. Furthermore, these mechanically stressed fibers still have the capacity for saturation with water, methylene blue dye, crude oil, and blood, which is necessary for the application of such renewable 3D chitinous centimeter-sized scaffolds in diverse technological and biomedical fields.

Keywords: chitin; marine sponges; scaffolds; Aplysina archeri; express method; bromotyrosines; crude oil; blood; methylene blue

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MDPI and ACS Style

Klinger, C.; Żółtowska-Aksamitowska, S.; Wysokowski, M.; Tsurkan, M.V.; Galli, R.; Petrenko, I.; Machałowski, T.; Ereskovsky, A.; Martinović, R.; Muzychka, L.; et al. Express Method for Isolation of Ready-to-Use 3D Chitin Scaffolds from Aplysina archeri (Aplysineidae: Verongiida) Demosponge. Mar. Drugs 2019, 17, 131. https://doi.org/10.3390/md17020131

AMA Style

Klinger C, Żółtowska-Aksamitowska S, Wysokowski M, Tsurkan MV, Galli R, Petrenko I, Machałowski T, Ereskovsky A, Martinović R, Muzychka L, et al. Express Method for Isolation of Ready-to-Use 3D Chitin Scaffolds from Aplysina archeri (Aplysineidae: Verongiida) Demosponge. Marine Drugs. 2019; 17(2):131. https://doi.org/10.3390/md17020131

Chicago/Turabian Style

Klinger, Christine, Sonia Żółtowska-Aksamitowska, Marcin Wysokowski, Mikhail V. Tsurkan, Roberta Galli, Iaroslav Petrenko, Tomasz Machałowski, Alexander Ereskovsky, Rajko Martinović, Lyubov Muzychka, and et al. 2019. "Express Method for Isolation of Ready-to-Use 3D Chitin Scaffolds from Aplysina archeri (Aplysineidae: Verongiida) Demosponge" Marine Drugs 17, no. 2: 131. https://doi.org/10.3390/md17020131

APA Style

Klinger, C., Żółtowska-Aksamitowska, S., Wysokowski, M., Tsurkan, M. V., Galli, R., Petrenko, I., Machałowski, T., Ereskovsky, A., Martinović, R., Muzychka, L., Smolii, O. B., Bechmann, N., Ivanenko, V., Schupp, P. J., Jesionowski, T., Giovine, M., Joseph, Y., Bornstein, S. R., Voronkina, A., & Ehrlich, H. (2019). Express Method for Isolation of Ready-to-Use 3D Chitin Scaffolds from Aplysina archeri (Aplysineidae: Verongiida) Demosponge. Marine Drugs, 17(2), 131. https://doi.org/10.3390/md17020131

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Express Method for Isolation of Ready-to-Use 3D Chitin Scaffolds from Aplysina archeri (Aplysineidae: Verongiida) Demosponge

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