Echinochrome A Promotes Ex Vivo Expansion of Peripheral Blood-Derived CD34+ Cells, Potentially through Downregulation of ROS Production and Activation of the Src-Lyn-p110δ Pathway
Abstract
:1. Introduction
2. Results
2.1. Ech A Suppresses ROS Production and Promotes Expansion of PBMC-Derived CD34+ Cells
2.2. Ech A Inhibits the Activation of p38-MAPK and JNK in PB-CD34+ Cells
2.3. Ech A Regulates PB-CD34+ Cell Expansion via PI3K/Akt Pathway
2.4. The PI3K p110δ Isoform Is Required for Ech A-Induced CD34+ Cell Expansion
2.5. Src/Lyn Is a Major Upstream Signal for Ech A-Induced p110δ-Mediated CD34+ Cell Expansion
2.6. Ex Vivo Expanded CD34+ Cells Maintain Their Colony-Forming Capacity
3. Discussion
4. Materials and Methods
4.1. Isolation of PBMCs and Purification of PB-CD34+ Cells
4.2. Chemicals and Reagents
4.3. Immunophenotypic Analysis by Flow Cytometry
4.4. Determination of Intracellular ROS Production
4.5. Immunoblotting
4.6. Colony-Forming Cell (CFC) Assay
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Park, G.-B.; Kim, M.-J.; Vasileva, E.A.; Mishchenko, N.P.; Fedoreyev, S.A.; Stonik, V.A.; Han, J.; Lee, H.S.; Kim, D.; Jeong, J.-Y. Echinochrome A Promotes Ex Vivo Expansion of Peripheral Blood-Derived CD34+ Cells, Potentially through Downregulation of ROS Production and Activation of the Src-Lyn-p110δ Pathway. Mar. Drugs 2019, 17, 526. https://doi.org/10.3390/md17090526
Park G-B, Kim M-J, Vasileva EA, Mishchenko NP, Fedoreyev SA, Stonik VA, Han J, Lee HS, Kim D, Jeong J-Y. Echinochrome A Promotes Ex Vivo Expansion of Peripheral Blood-Derived CD34+ Cells, Potentially through Downregulation of ROS Production and Activation of the Src-Lyn-p110δ Pathway. Marine Drugs. 2019; 17(9):526. https://doi.org/10.3390/md17090526
Chicago/Turabian StylePark, Ga-Bin, Min-Jung Kim, Elena A. Vasileva, Natalia P. Mishchenko, Sergey A. Fedoreyev, Valentin A. Stonik, Jin Han, Ho Sup Lee, Daejin Kim, and Jee-Yeong Jeong. 2019. "Echinochrome A Promotes Ex Vivo Expansion of Peripheral Blood-Derived CD34+ Cells, Potentially through Downregulation of ROS Production and Activation of the Src-Lyn-p110δ Pathway" Marine Drugs 17, no. 9: 526. https://doi.org/10.3390/md17090526