Involvement of Reactive Oxygen Species in the Hepatorenal Toxicity of Actinomycin V In Vitro and In Vivo
Abstract
:1. Introduction
2. Results
2.1. Comparing Cytotoxicity of Act V and Act D in LO-2 and 293T Cells
2.2. Effect of Act V and Act D on Organ to Body Weight Ratios in Mice
2.3. Effect of Act V and Act D on Liver and Kidney Histopathology
2.4. Effect of Act V and Act D on Liver and Kidney Indicators in Mouse Serum
2.5. Act V and Act D Promote the Generation of ROS in LO-2 and 293T Cells
2.6. Act V and Act D Accelerate Lipid Oxidation in LO-2 And 293T Cells
2.7. Act V and Act D Reduce Radical Scavengers Levels in LO-2 and 293T Cells
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Animals
4.3. Treatments and Sample Collection
4.4. Histopathological Examination
4.5. Estimation of Liver and Kidney Function Markers in Mouse Serum
4.6. Cell Lines
4.7. MTT Assay
4.8. Flow Cytometric Analysis of ROS Levels
4.9. Measurement of MDA, GSH and SOD Content
4.10. Statistical Analysis
Author Contributions
Funding
Conflicts of Interest
References
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Cell Lines IC50 (μmol/L) | Act V | Act D |
---|---|---|
LO-2 | 0.0828 ± 0.0015 | 0.0382 ± 0.0015 |
293T | 0.0750 ± 0.0008 | 0.0675 ± 0.0025 |
A549 | 0.0068 ± 0.0006 | 0.1284 ± 0.0072 |
MDA-MB-231 | 0.0083 ± 0.0032 | 0.1515 ± 0.0052 |
K562 | 0.0046 ± 0.0001 | 0.1272 ± 0.0033 |
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Jia, F.-j.; Han, Z.; Ma, J.-h.; Jiang, S.-q.; Zhao, X.-m.; Ruan, H.; Xie, W.-d.; Li, X. Involvement of Reactive Oxygen Species in the Hepatorenal Toxicity of Actinomycin V In Vitro and In Vivo. Mar. Drugs 2020, 18, 428. https://doi.org/10.3390/md18080428
Jia F-j, Han Z, Ma J-h, Jiang S-q, Zhao X-m, Ruan H, Xie W-d, Li X. Involvement of Reactive Oxygen Species in the Hepatorenal Toxicity of Actinomycin V In Vitro and In Vivo. Marine Drugs. 2020; 18(8):428. https://doi.org/10.3390/md18080428
Chicago/Turabian StyleJia, Fu-juan, Zhuo Han, Jia-hui Ma, Shi-qing Jiang, Xing-ming Zhao, Hang Ruan, Wei-dong Xie, and Xia Li. 2020. "Involvement of Reactive Oxygen Species in the Hepatorenal Toxicity of Actinomycin V In Vitro and In Vivo" Marine Drugs 18, no. 8: 428. https://doi.org/10.3390/md18080428