Influence of Fucoidan Extracts from Different Fucus Species on Adult Stem Cells and Molecular Mediators in In Vitro Models for Bone Formation and Vascularization
, Signe Helle Ptak 3,†, Ramona Römer 1, Junyu Xiong 1, Julia Ohmes 1, Andreas Seekamp 1, Xavier Fretté 3, Susanne Alban 2 and Sabine Fuchs 1,*Round 1
Reviewer 1 Report
The manuscript entitled "Influence of fucoidan extracts from different Fucus species on adult stem cells and molecular mediators involved in bone formation and vascularization" addresses a very relevant topic of bone formation and vascularization from stem cells subjected to different fucoidan extracts extracted from brown algae of the genus Fucus. This manuscript is very well structured and well written. In order to improve the manuscript, the authors must make a correction at the level of the algae taxonomy and other small corrections to standardize the text.
Corrections needed:
line 22 - Fucus (in itlaics)
line 23 - Fucus vesiculosus (Fv), Fucus serratus (Fs) and Fucus distichus subsp. evanescens (in italics)
line 23 - Fucus distichus subsp. evanescens (formerly Fucus evanescens)
line 62 - in vitro (in italics)
line 73 - Fucus (in italics)
line 80 - F. vesiculosus, F. distichus subsp. evanescens and F. serratus (in italics)
line 89 - Figure 1 and 2
line 104 and Table 2 - Fucus vesiculosus (in italics, twice)
line 123 - from Fucus vesiculosus (in italics) (Figure 2).
line 125 - (Figure 2, arrow),...
line 131 - (Figure 3)
line 136 - Fucus vesiculosus (in italics)
line 162 - Fucus (in italics)
line 166 - (Figures 5 a and b)
line 169 - (Figures 5 c and d)
line 179 - Figures 6 a and b
line 185/186 - Figures 6 c and d, ...
line 191 - cells/cm2
line 194 - Fucus (in italics)
line 201 (Figure 7b).
line 202 - Figure 7d, ...
line 205 - (Figure 7c).
line 234 - Standardize the units throughout the text: mL or ml
line 244 - Fucus (in italics)
line 246 - (Figures 1 and 5) ...
line 248 - (Figures 4 and 8).
line 250 - (Figure 9).
line 255/256 and Figure 9 - Standardize the units throughout the text: mL or ml
line 283 - treatment at 120 and 100 °C, respectively,...
line 286 - (Tables 1 and S1).
line 341/342 - ...from Fucus distichus subsp. evanescence resulted...
line 376 - F. vesiculosus (Fv), F. serratus (Fs), and F. distichus subsp. evanescens (Fe) were...
line 395 - F. vesiculosus (in italics)
line 396 - 24 h, followed by ...
line 396 - hydrochloric acid for 24 h, followed by ...
line 397 - Fucus vesiculosus (in italics)
line 399 - Fucus vesiculosus (in italics)
line 401 - F. distichus subsp. evanescens (in italics)
line 402 - Table 3.
line 403 and Table 3 - F. vesiculosus, F. distichus subsp. evanescens, F. serratus (in italics)... 24 h (Twice)
line 456 - cells/cm2
line 472 - cells/cm2
line 477 - cells/cm2
line 488 - cells/cm2
line 508 - cells/cm2
line 513 - albumin (BSA) for 1.5 h and ...
line 515 - Standardize the units throughout the text: mL or ml
line 521 - cells/cm2
line 525 - cells/cm2
line 565 - Standardize the units throughout the text: mL or ml
line 566 - Standardize the units throughout the text: mL or ml
line 585 - cells/cm2
line 589 - Standardize the units throughout the text: mL or ml
Author Response
Reviewer 1:
The manuscript entitled "Influence of fucoidan extracts from different Fucus species on adult stem cells and molecular mediators involved in bone formation and vascularization" addresses a very relevant topic of bone formation and vascularization from stem cells subjected to different fucoidan extracts extracted from brown algae of the genus Fucus. This manuscript is very well structured and well written. In order to improve the manuscript, the authors must make a correction at the level of the algae taxonomy and other small corrections to standardize the text.
Corrections needed: formal corrections as listed by the reviewer.
We would like to thank the reviewer for the very careful revision of formal issues within the manuscript.
The format has been adjusted in accordance with the reviewer’s suggestion.
Reviewer 2 Report
The article entitled “ Influence of fucoidan extracts from different Fucus species on adult stem cells and molecular mediators involved in bone formation and vascularization” prepared by
Fanlu Wang et al. is clearly presented. The methodology is adequate and the results acutely analyzed.
All the results and the possible used of Fucus species as treatment are based on in vitro experiments. The authors should indicate that it is in vitro study in the title. As well they should discuss in vivo experiments have to be conducted before the fucus derivatives may be used as drugs.
Author Response
Reviewer 2:
The article entitled “ Influence of fucoidan extracts from different Fucus species on adult stem cells and molecular mediators involved in bone formation and vascularization” prepared by
Fanlu Wang et al. is clearly presented. The methodology is adequate and the results acutely analyzed.
All the results and the possible used of Fucus species as treatment are based on in vitro experiments. The authors should indicate that it is in vitro study in the title. As well they should discuss in vivo experiments have to be conducted before the fucus derivatives may be used as drugs.
We thank the author for this suggestion. We changed the title and the conclusions in accordance with this remark. We are totally aware that we provide in vitro data in this study which need to be further supported in vitro study. Our study should help to select extracts with a biomedical potential for further in vivo studies.
