New Chlorinated Metabolites and Antiproliferative Polyketone from the Mangrove Sediments-Derived Fungus Mollisia sp. SCSIO41409
, Xia Gan 3,4, Qian Zhou 3, Xiaowei Luo 4, Bin Yang 1,2, Yonghong Liu 1,2, Disna Ratnasekera 5,* and Xuefeng Zhou 1,2,*
Round 1
Reviewer 1 Report
The manuscript entitled (New Chlorinated Metabolites and Antiproliferative Polyketone from the Mangrove Sediments-Derived Fungus Mollisia sp. SCSIO41409) by Cai et al. reported the isolation, purification, and structure elucidation of two chlorinated metabolites and eight known compounds were isolated from the mangrove sediments-derived fungus Mollisia sp. SCSIO41409. Their structures were elucidated by physicochemical properties and extensive spectroscopic analysis. The absolute configuration of stemphone C was established for the first time by the X-ray crystallographic analysis. All the obtained compounds were evaluated for the activities of pathogenic fungi and bacteria commonly found in crop plants. Compounds 3 and 4 were tested for their two human prostate cancer cell lines, PC-3 (androgen receptor negative) and 22Rv1 (androgen receptor positive).
The manuscript is good, and it could be accepted after covering the following issues
1- Different English errors should be checked and correct.
2- In introduction authors should add previously metabolites isolated from the fungus, along with their bioactivity.
Different paper on the fungus have been previously reported as:
A- Mollisianitrile, a New Antibiotic from Mollisia sp. A59Ð96.
B- New metabolites from the endophytic fungus Mollisia sp.
3- Meting point for new compounds should be measured and added in experimental section.
4- Authors evaluated all compounds as antimicrobial activites. But for cytotoxicity chose compounds 3 and 4 only, please clarify and give your reasons (Whey).
5- In supplementary material please capitalize the first letter for the name of each compound as; stemphone C should write as Stemphone C
Author Response
Please see the attachment.
Author Response File: 
Author Response.pdf
Reviewer 2 Report
Review manuscript: marinedrugs-2108902
Title: New Chlorinated Metabolites and Antiproliferative Polyketone from the Mangrove Sediments-Derived Fungus Mollisia sp. SCSIO41409
1 1) Comments: Page 2, Results:
Line 69: Compound 1: HMBC correlation from H3-9 to C-4 are long range correlation. There is no any long range correlation from H3-9 to C8a to confirm the localization of methyl groups CH3-9 at C-2?
2 2) Comments: Page 4, Results:
Line 107: Compound 1: On Table 1, you forget to report the long range correlation from H3-9 to C-4 as you mentioned in the manuscript.
3) Comments: Page 6, Supporting Information:
Figure S4: is HMBC spectrum of 1 in DMSO-d6.
Figure S5: is HSQC spectrum of 1 in DMSO-d6
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
Reviewer 3 Report
The manuscript “New Chlorinated Metabolites and Antiproliferative Polyketone from the Mangrove Sediments-Derived Fungus Mollisia sp. SCSIO41409” reported the isolation and structural elucidation of two new chlorinated metabolites, 8-chlorine-5-hydroxy-2,3-dimethyl-7-methoxychromone (1) and 3,4-dichloro-1H-pyrrole-2,5-dione (3), together with eight known compounds (2 and 4−9) from the mangrove sediments-derived fungus Mollisia sp. SCSIO41409. The structure of 3 and absolute configuration of 4 were determined by X-ray spectroscopic analysis. Compounds 3 showed good antifungal and antiproliferative activities against 22Rv-1 and PC3. Interestingly, Stemphone C (4) show most significant activity on PC-3 cells, induced apoptosis, and blocked the cell cycle at S phase in a dose-dependent manner. The manuscript was prepared carefully. The results of study are sound.
I recommend the manuscript will be accepted after minor revisions.
1. Provide the information on fermentation and extraction of fungus material.
Author Response
Response: Fermentation and extraction have been added.
“4.3. Fermentation and Extraction
The fungal strain was cultured in 200 mL seed medium (1.5% malt extract, 1.5% sea salt) in 500 mL Erlenmeyer flasks at 28 ℃ for 3 days on a rotary shaker (180 rpm). A largescale fermentation was incubated statically at 26 ℃ for 60 days using a rice medium (150 g rice, 1.5% sea salt, 150 mL H2O) in the 1 L flask (× 53). The whole fermented culture was extracted with EtOAc three times to afford a brown extract (197.2 g).”
Round 2
Reviewer 1 Report
No Comments
