Nutritional Value and Productivity Potential of the Marine Microalgae Nitzschia laevis, Phaeodactylum tricornutum and Isochrysis galbana
Abstract
:1. Introduction
2. Results and Discussion
2.1. Growth Characteristics and Carbon Partitioning of P. tricornutum, I. galbana and N. laevis
2.2. Amino Acid Composition of P. tricornutum, I. galbana and N. laevis
2.3. Monosaccharide Profiles of P. tricornutum, I. galbana and N. laevis
2.4. Fatty Acids Profiles of P. tricornutum, I. galbana and N. laevis
2.5. Fucoxanthin and PUFAs Productivities of P. tricornutum, I. galbana and N. laevis
3. Materials and Methods
3.1. Strains and Mediums
3.2. Culture Conditions
3.3. Determination of Microalgal Biomass
3.4. Analysis of Protein and Amino Acids
3.5. Analysis of Carbohydrates and Monosac Charides
3.6. Analysis of Lipids and Fatty Acids
3.7. Analysis of Pigments and Fucoxanthin
3.8. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Carbon Partitioning Content (%DCW) b | P. tricornutum | I. galbana | N. laevis |
---|---|---|---|
Protein | 43.29 ± 0.76 | 31.60 ± 0.15 | 32.28 ± 0.25 |
Carbohydrate | 14.85 ± 1.12 | 25.40 ± 1.38 | 21.97 ± 0.84 |
Lipid | 23.56 ± 0.61 | 26.84 ± 0.70 | 21.25 ± 0.53 |
Pigment | 7.31 ± 0.43 | 3.07 ± 0.22 | 2.45 ± 0.17 |
Amino Acid b | FAO/WHO Standard (2013) | Amino Acid Content (mg/g) | ||
---|---|---|---|---|
P. tricornutum | I. galbana | N. laevis | ||
Non-essential Amino Acid (NEAA) | ||||
Gly | 2.89 ± 011 | 3.32 ±0.04 | 2.20 ± 0.15 | |
Ala | 4.62 ± 0.09 | 6.39 ±0.06 | 3.50 ± 0.18 | |
Ser | 3.16 ± 0.08 | 3.83 ±0.01 | 3.19 ± 0.24 | |
Cys | 0.53 ± 0.01 | 0.70 ±0.01 | 0.53 ± 0.01 | |
Asp | 7.90 ± 0.23 | 9.11 ±0.09 | 6.38 ± 0.31 | |
Asn | 0.05 ± 0.00 | 0.06 ±0.00 | 0.03 ± 0.00 | |
Glu | 8.48 ± 0.23 | 12.37 ± 0.01 | 7.78 ± 0.41 | |
Gln | 0.49 ± 0.03 | 0.16 ± 0.00 | 0.09 ± 0.02 | |
Tyr | 0.83 ± 0.04 | 1.36 ± 0.08 | 0.87 ± 0.01 | |
Pro | 2.75 ± 0.04 | 3.61 ± 0.03 | 1.95 ± 0.10 | |
Arg | 4.30 ± 0.08 | 4.75 ± 0.16 | 4.21 ± 0.23 | |
Essential amino acid (EAA) | ||||
Val | 4.00 | 3.51 ± 0.03 | 4.68 ± 0.07 | 2.85 ± 0.13 |
Thr | 2.50 | 3.30 ± 0.12 | 4.11 ± 0.04 | 2.73 ± 0.17 |
Ile | 3.00 | 3.33 ± 0.10 | 3.70 ± 0.08 | 2.23 ± 0.14 |
Met | 1.02 ± 0.02 | 1.46 ± 0.05 | 0.74 ± 0.06 | |
His | 1.60 | 1.22 ± 0.01 | 1.68 ± 0.01 | 0.90 ± 0.06 |
Phe | 3.47 ± 0.06 | 3.80 ± 0.01 | 2.01 ± 0.11 | |
Trp | 0.66 | 0.03 ± 0.00 | ||
Lys | 4.80 | 2.73 ± 0.17 | 2.82 ± 0.03 | 2.35 ± 0.08 |
Leu | 6.10 | 5.57 ± 0.06 | 7.28 ± 0.03 | 4.03 ± 0.13 |
Phe + Tyr | 4.10 | 4.30 ± 0.10 | 5.16 ± 0.09 | 2.88 ± 0.12 |
Met + Cys | 2.3 | 1.55 ± 0.03 | 2.15 ± 0.06 | 1.27 ± 0.07 |
EAA/TAA | 40.15 ± 1.06 | 39.29 ± 0.89 | 36.73 ± 2.99 | |
EAA/NEAA | 67.07 ± 2.33 | 64.73 ± 1.67 | 58.06 ± 4.14 | |
EAAI | 88.00 ± 4.67 | 77.30 ± 0.66 | 67.03 ± 6.17 |
Sugar | Abbreviation | Monosaccharide Content (mg/g) | ||
---|---|---|---|---|
P. tricornutum | I. galbana | N. laevis | ||
Guluronic acid | GulA | 10.89 ± 0.81 | 6.45 ± 0.78 | 2.27 ± 0.01 |
Mannuronic acid | ManA | 0.72 ± 0.02 | 0.73 ± 0.03 | |
Mannose | Man | 41.16 ± 3.02 | 5.25 ± 0.45 | 44.26 ± 4.80 |
Ribose | Rib | 2.96 ± 0.33 | 3.45 ± 0.34 | 4.25 ± 0.37 |
Rhamnose | Rham | 3.49 ± 0.23 | 0.35 ± 0.02 | 1.33 ± 0.06 |
Glucosamine | GlcN | 1.25 ± 0.08 | 0.63 ± 0.05 | 2.25 ± 0.04 |
Glucuronic acid | GlcUA | 15.44 ± 0.55 | 4.79 ± 0.15 | 4.84 ± 0.13 |
Galacturonic acid | GalUA | 1.00 ± 0.06 | 1.16 ± 0.08 | 2.85 ± 0.17 |
Galactosamine | GalN | 0.21 ± 0.09 | ||
Glucose | Glc | 257.58 ± 13.30 | 38.11 ± 1.16 | 529.10 ± 2.55 |
Galactose | Gal | 28.70 ± 1.22 | 23.98 ± 2.38 | 10.94 ± 0.95 |
Xylose | Xyl | 6.46 ± 0.55 | 3.31 ± 0.18 | 3.60 ± 0.23 |
Arabinose | Ara | 0.75 ± 0.05 | 7.01 ± 0.67 | 1.21 ± 0.11 |
Fucose | Fuc | 1.72 ± 0.17 | 3.39 ± 0.29 | 2.24 ± 0.28 |
Fatty Acid | Fatty Acid Content (%DCW) | ||
---|---|---|---|
P. tricornutum | I. galbana | N. laevis | |
C14:0 | 0.98 ± 0.00 | 1.71 ± 0.01 | 0.83 ± 0.10 |
C16:0 | 1.57 ± 0.01 | 1.82 ± 0.02 | 1.36 ± 0.12 |
C16:1 | 3.41 ± 0.01 | 0.32 ± 0.01 | 2.89 ± 0.12 |
C16:2 | 0.32 ± 0.01 | 0.45 ± 0.00 | |
C16:3 | 0.50 ± 0.01 | ||
C18:1 | 0.65 ± 0.00 | 3.17 ± 0.03 | 0.19 ± 0.00 |
C18:2n-6 | 0.27 ± 0.01 | 0.29 ± 0.00 | 0.19 ± 0.00 |
C18:3n-3/6 | 1.05 ± 0.01 | 0.30 ± 0.01 | |
C18:4n-3 | 1.20 ± 0.03 | 0.13 ± 0.01 | |
C20:4n-6 | 0.22 ± 0.00 | 0.33 ± 0.02 | |
C20:5n-3 | 2.17 ± 0.03 | 2.18 ± 0.14 | |
C22:6n-3 | 0.91 ± 0.01 | 0.18 ± 0.00 | |
others | 0.22 ± 0.00 | 0.28 ± 0.02 | 0.65 ± 0.01 |
TFA | 10.09 ± 0.06 | 10.95 ± 0.02 | 9.66 ± 0.22 |
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Lu, X.; Yang, S.; He, Y.; Zhao, W.; Nie, M.; Sun, H. Nutritional Value and Productivity Potential of the Marine Microalgae Nitzschia laevis, Phaeodactylum tricornutum and Isochrysis galbana. Mar. Drugs 2024, 22, 386. https://doi.org/10.3390/md22090386
Lu X, Yang S, He Y, Zhao W, Nie M, Sun H. Nutritional Value and Productivity Potential of the Marine Microalgae Nitzschia laevis, Phaeodactylum tricornutum and Isochrysis galbana. Marine Drugs. 2024; 22(9):386. https://doi.org/10.3390/md22090386
Chicago/Turabian StyleLu, Xue, Shufang Yang, Yongjin He, Weixuan Zhao, Man Nie, and Han Sun. 2024. "Nutritional Value and Productivity Potential of the Marine Microalgae Nitzschia laevis, Phaeodactylum tricornutum and Isochrysis galbana" Marine Drugs 22, no. 9: 386. https://doi.org/10.3390/md22090386