Genome Analysis of a Potential Novel Vibrio Species Secreting pH-and Thermo-Stable Alginate Lyase and Its Application in Producing Alginate Oligosaccharides
Abstract
:1. Introduction
2. Results
2.1. Screening of Strain HB236076
2.2. Genome Specifics
2.3. Identification of Strain HB236076
2.4. Genetic Basis of Alginate Degradation
2.5. Detection of Activity and Properties of Alginate Lyase
2.6. Enzymatic Degradation of Sodium Alginate
2.7. Antimicrobial Activity of Alginate Oligosaccharides
3. Materials and Methods
3.1. Materials and Strains
3.2. Screening of Alginate-Degrading Bacteria
3.3. Phylogenetic Analysis Based on 16S rRNA Gene
3.4. Genome Sequencing and Annotation
3.5. Detection of Alginate Lyase Activity and Enzymatic Properties
3.6. Preparation and Detection of the Degradation Products of the Alginate Lyase
3.7. Assays for Antimicrobial Activity
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Enzymes | Accession No. | PL Family | Length (aa) | MW (Dal) | Signal Protein | Sequence Comparison 1 | ||
---|---|---|---|---|---|---|---|---|
PI | Sequence Identity (%) | Reference Alginate Lyase | ||||||
Alg1 | XDK25489.1 | PL 7 | 279 | 30,235.57 | Yes | 3.90 | 61.9 | GAA4099934.1 |
Alg2 | XDK26069.1 | PL 7 | 652 | 71,934.82 | Yes | 5.66 | 79.6 | WP_231565442.1 |
Alg3 | XDK26066.1 | PL 7 | 343 | 38,020.27 | Yes | 6.17 | 76.0 | WP_102470889.1 |
Alg4 | XDK25655.1 | PL 7 | 285 | 32,002.96 | Yes | 6.80 | 97.2 | ABB36771.1 |
Alg5 | XDK24838.1 | PL 7 | 248 | 28,306.44 | Yes | 4.50 | 97.0 | ABB36772.1 |
Alg6 | XDK24840.1 | PL 7 | 342 | 37,836.15 | Yes | 6.60 | 60.6 | WP_334550621.1 |
Alg7 | XDK24841.1 | PL 7 | 302 | 33,260.67 | Yes | 4.51 | 47.9 | WP_318074641.1 |
Alg8 | XDK24833.1 | PL 17 | 720 | 80,516.22 | No | 5.39 | 65.1 | CAH6913425.1 |
Alg9 | XDK24834.1 | PL 17 | 720 | 80,465.18 | No | 5.68 | 63.2 | WP_252024937.1 |
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Bao, K.; Yang, M.; Sun, Q.; Zhang, K.; Huang, H. Genome Analysis of a Potential Novel Vibrio Species Secreting pH-and Thermo-Stable Alginate Lyase and Its Application in Producing Alginate Oligosaccharides. Mar. Drugs 2024, 22, 414. https://doi.org/10.3390/md22090414
Bao K, Yang M, Sun Q, Zhang K, Huang H. Genome Analysis of a Potential Novel Vibrio Species Secreting pH-and Thermo-Stable Alginate Lyase and Its Application in Producing Alginate Oligosaccharides. Marine Drugs. 2024; 22(9):414. https://doi.org/10.3390/md22090414
Chicago/Turabian StyleBao, Ke, Miao Yang, Qianhuan Sun, Kaishan Zhang, and Huiqin Huang. 2024. "Genome Analysis of a Potential Novel Vibrio Species Secreting pH-and Thermo-Stable Alginate Lyase and Its Application in Producing Alginate Oligosaccharides" Marine Drugs 22, no. 9: 414. https://doi.org/10.3390/md22090414