Violacein-Producing Collimonas sp. from the Sea Surface Microlayer of Costal Waters in Trøndelag, Norway
Abstract
:1. Introduction
2. Results and Discussion
2.1. Isolation of Collimonas CT
2.2. Identification and characterization of antimicrobial compound and pigment
2.3. Violacein biosynthesis genes in Collimonas CT
3. Experimental Section
3.1. Sampling sites and sample collection
3.2. Preparation of bacterial inoculums
3.3. Culture condition for production and extraction of secondary metabolites
3.4. Antimicrobial assay
3.5. Fractionation and LC-MS-TOF analysis of bacterial extracts
3.6. Cloning, sequencing and phylogenetic analysis
Acknowledgments
- Samples Availability: Available from the authors.
References and Notes
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Strain or plasmid | Description | Source or reference |
---|---|---|
Candida albicans | Cyhr AmBs, Hps, Nyss † | ATCC• (strain 10231) |
Micrococcus luteus | Amoxs, Amps, Cms, Ccls, Nbs, Oles, Pens, Pcns, Rifs, Tys £ | ATCC• (strain 9341) |
E. coli K12 | ||
Enterococcus faecium | Ampr, Ctcr, Eryr, Lcmr, Vcmr, Amr, Bacr, Csr, Spr § | CCUG* (strain 37832) |
Enterococcus faecium Collimonas CT | Amr, Bacr, Csr, Spr | CTC (strain 492) This study |
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Hakvåg, S.; Fjærvik, E.; Klinkenberg, G.; Borgos, S.E.F.; Josefsen, K.D.; Ellingsen, T.E.; Zotchev, S.B. Violacein-Producing Collimonas sp. from the Sea Surface Microlayer of Costal Waters in Trøndelag, Norway. Mar. Drugs 2009, 7, 576-588. https://doi.org/10.3390/md7040576
Hakvåg S, Fjærvik E, Klinkenberg G, Borgos SEF, Josefsen KD, Ellingsen TE, Zotchev SB. Violacein-Producing Collimonas sp. from the Sea Surface Microlayer of Costal Waters in Trøndelag, Norway. Marine Drugs. 2009; 7(4):576-588. https://doi.org/10.3390/md7040576
Chicago/Turabian StyleHakvåg, Sigrid, Espen Fjærvik, Geir Klinkenberg, Sven Even F. Borgos, Kjell D. Josefsen, Trond E. Ellingsen, and Sergey B. Zotchev. 2009. "Violacein-Producing Collimonas sp. from the Sea Surface Microlayer of Costal Waters in Trøndelag, Norway" Marine Drugs 7, no. 4: 576-588. https://doi.org/10.3390/md7040576