Encapsidated Host Factors in Alphavirus Particles Influence Midgut Infection of Aedes aegypti
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Lines
2.2. Virus Preparation and Purification
2.3. Virus Particle Quantification
2.4. Analysis of Viral Glycoproteins
2.5. Mosquito Infections
2.6. Quantitative Analysis of Mosquito Transcripts
2.7. Statistical Analysis
3. Results
3.1. Alphaviruses Produce Distinct Infectious Populations
3.2. Per Os Infections with Viral Subpopulations
3.3. Bypassing the Midgut Barriers
3.4. Determining the Midgut Immune Response
4. Discussion
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Primer | Sequence |
---|---|
18s F | CGAAAGTTAGAGGTTCGAAGGCGA |
18s R | CCGTGTTGAGTCAAATTAAGCCGC |
nsP1 F | AAGGATCTCCGGACCGTA |
nsP1 R | AACATGAACTGGGTGTCGAAG |
AeDif F | ACAAACGTCTCCCTACAATG |
AeDif R | ACTGATTCTGGAACTGTTGG |
Rel2 F | CAAGAACAGGAAGAGAAC |
Rel2 R | CCTCCACTCTATTACAGC |
AeDronc F | AAAGAACTGAAGCAGTCCAG |
AeDronc R | CGTATAGGACGGAATTATCG |
Ago2 F | ACCGAATATCACCTTCATTG |
Ago2 R | TAACGGTCAACGATAGTTCC |
AeSTAT F | GTTTCGATCATTTCAGAAGC |
AeSTAT R | CTTCGTGGTTTCGTTGTACT |
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Mackenzie-Liu, D.; Sokoloski, K.J.; Purdy, S.; Hardy, R.W. Encapsidated Host Factors in Alphavirus Particles Influence Midgut Infection of Aedes aegypti. Viruses 2018, 10, 263. https://doi.org/10.3390/v10050263
Mackenzie-Liu D, Sokoloski KJ, Purdy S, Hardy RW. Encapsidated Host Factors in Alphavirus Particles Influence Midgut Infection of Aedes aegypti. Viruses. 2018; 10(5):263. https://doi.org/10.3390/v10050263
Chicago/Turabian StyleMackenzie-Liu, David, Kevin J. Sokoloski, Sarah Purdy, and Richard W. Hardy. 2018. "Encapsidated Host Factors in Alphavirus Particles Influence Midgut Infection of Aedes aegypti" Viruses 10, no. 5: 263. https://doi.org/10.3390/v10050263