MultiBac: Baculovirus-Mediated Multigene DNA Cargo Delivery in Insect and Mammalian Cells
, Christine Tölzer 1,2, Duygu Sari-Ak 3, Daniel J. Fitzgerald 4, Christiane Schaffitzel 1,2 and Imre Berger 1,2,*Round 1
Reviewer 1 Report
In this review manuscript, Gupta et al summarized the capacity of the MultiBac baculovirus protein production system developed by the group for commercial application of multiprotein expression in insect cells. The manuscript is well organized and can be followed with ease.
However, this manuscript is more like an advertisement for MutliBac. If this is the way Viruses want, I am fine with it.
Author Response
We thank the Reviewer for the very positive assessment of our manuscript.
Reviewer 2 Report
The review paper was very interesting to read and discussed a number of applications of the MultiBac system in insect and mammalian cells. It describes a number of different studies which have used the system for the expression of multiple genes, and provides sufficient background information and/or references for a reader to delve further into a given example. As such, only a few minor corrections are suggested.
Minor corrections:
Line 92: Figure 1 uses the abbreviation KanR but the legend has a different abbreviation of Kn. Please correct this.
Line 92: Figure 1 does not have the abbreviation Chl (Chloramphenicol) and this should instead be presented in figure 5 where the abbreviation ChlR is used.
Line 93: Figure 1 does not use the abbreviation OriR6ky and this should instead be presented in figure 5 where the abbreviation is used.
Author Response
We thank the reviewer for the very positive assessment of our manuscript. We have included all suggestions and corrections in our revised manuscript:
Line 92: Figure 1 uses the abbreviation KanR but the legend has a different abbreviation of Kn. Please correct this.
Corrected.
Line 92: Figure 1 does not have the abbreviation Chl (Chloramphenicol) and this should instead be presented in figure 5 where the abbreviation ChlR is used.
Corrected.
Line 93: Figure 1 does not use the abbreviation OriR6ky and this should instead be presented in figure 5 where the abbreviation is used.
Corrected.
We have also removed typos we had discovered in the meantime in the manuscript and made an addition to the Acknowledgments, thanking Dale Wigley (Imperial College, London) for sharing of data.
