Histone Deacetylase Inhibitors Enhance Cell Killing and Block Interferon-Beta Synthesis Elicited by Infection with an Oncolytic Parainfluenza Virus
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells, Viruses, and Infections
2.2. Chemical Preparation
2.3. Cell Viability and Caspase Assays
2.4. Western Blotting
2.5. Fluorescence Microscopy and IRF-3 immunostaining
2.6. Human IFN-β ELISA
2.7. Reverse Transcription and Real Time PCR
2.8. Statistical Analyses
3. Results
3.1. HDAC Inhibitors Enhance Killing of Lung Cancer Cells by the P/V-CPI- Mutant through Increases in Caspase Activity
3.2. Double Stranded RNA Contributes to scriptaid-Mediated Enhancement of Cell Killing by the P/V-CPI- Virus
3.3. Scriptaid Pretreatment Enhances Killing of Lung Cancer Cells Infected with LACV and VSV
3.4. HDAC Inhibitor Pretreatment Downregulates IFN-β Production and Enhances Spread of the P/V-CPI- Mutant
3.5. Scriptaid Treatment Reduces P/V-CPI-Induced Nuclear Localization of IRF-3
3.6. Post-Infection Treatment of P/V-CPI-Infected Cells with a Panel of HDAC Inhibitors Reveals Two Cell Killing Profiles
4. Discussion
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Fox, C.R.; Parks, G.D. Histone Deacetylase Inhibitors Enhance Cell Killing and Block Interferon-Beta Synthesis Elicited by Infection with an Oncolytic Parainfluenza Virus. Viruses 2019, 11, 431. https://doi.org/10.3390/v11050431
Fox CR, Parks GD. Histone Deacetylase Inhibitors Enhance Cell Killing and Block Interferon-Beta Synthesis Elicited by Infection with an Oncolytic Parainfluenza Virus. Viruses. 2019; 11(5):431. https://doi.org/10.3390/v11050431
Chicago/Turabian StyleFox, Candace R., and Griffith D. Parks. 2019. "Histone Deacetylase Inhibitors Enhance Cell Killing and Block Interferon-Beta Synthesis Elicited by Infection with an Oncolytic Parainfluenza Virus" Viruses 11, no. 5: 431. https://doi.org/10.3390/v11050431
APA StyleFox, C. R., & Parks, G. D. (2019). Histone Deacetylase Inhibitors Enhance Cell Killing and Block Interferon-Beta Synthesis Elicited by Infection with an Oncolytic Parainfluenza Virus. Viruses, 11(5), 431. https://doi.org/10.3390/v11050431