Cellular microRNA-155 Regulates Virus-Induced Inflammatory Response and Protects against Lethal West Nile Virus Infection
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethics Statement
2.2. Animal Infections with WNV
2.3. ELISA and Multiplex Immunoassay
2.4. WNV Infection in Primary Mouse Cells
2.5. miRNA Overexpression in Human Neuroblastoma Cell Line, SK-N-SH
2.6. qRT-PCR
2.7. Statistical Analysis
3. Results
3.1. MicroRNA-155 Protects against Lethal WNV NY99 Infection
3.2. MicroRNA-155 is Required for Survival after Non-Lethal WNV Eg101 Challenge
3.3. miR-155 Modulates WNV Replication and Neuroinvasion
3.4. Antiviral Interferon Response is Altered in miR-155−/− Mice
3.5. miR-155 Regulates WNV-Induced Inflammatory Response
3.6. miR-155 Controls WNV Replication in Primary Mouse Cells
3.7. miR-155 Inhibits WNV Replication in Human Neuroblastoma Cells
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Gene (Accession No.) | Primer Sequence (5′-3′) |
---|---|
IL-1β (NM_000576) | |
Forward | AGCACCTTCTTTCCCTTCATC |
Reverse | GGACCAGACATCACCAAGC |
IL-6 (NM_000600) | |
Forward | CCAGGAGCCCAGCTATGAAC |
Reverse | CCCAGGGAGAAGGCAACTG |
IL-15 (NM_172175) | |
Forward | CGAAACCACATTTGAGAA |
Reverse | TGAAGGCATTAGTAGAGTAA |
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Natekar, J.P.; Rothan, H.A.; Arora, K.; Strate, P.G.; Kumar, M. Cellular microRNA-155 Regulates Virus-Induced Inflammatory Response and Protects against Lethal West Nile Virus Infection. Viruses 2020, 12, 9. https://doi.org/10.3390/v12010009
Natekar JP, Rothan HA, Arora K, Strate PG, Kumar M. Cellular microRNA-155 Regulates Virus-Induced Inflammatory Response and Protects against Lethal West Nile Virus Infection. Viruses. 2020; 12(1):9. https://doi.org/10.3390/v12010009
Chicago/Turabian StyleNatekar, Janhavi P., Hussin A. Rothan, Komal Arora, Philip G. Strate, and Mukesh Kumar. 2020. "Cellular microRNA-155 Regulates Virus-Induced Inflammatory Response and Protects against Lethal West Nile Virus Infection" Viruses 12, no. 1: 9. https://doi.org/10.3390/v12010009