Detection of Ebola Virus Antibodies in Fecal Samples of Great Apes in Gabon
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethics Statement
2.2. Sample Collection and RNA Isolation
2.3. EBOV, SUDV, BDBV, and MARV RNA Detection
2.4. Multiplexed Analysis of Anti-EBOV Antibodies in Great Ape Fecal Samples
2.4.1. Antigens
2.4.2. Recombinant Antigen Coupling to Microsphere
2.4.3. Multiplexed Immunoassay
2.5. Statistical Analysis
3. Results
3.1. No Viral RNA Detection
3.2. Feasibility of Antibody Detection in a Small Fecal Sample Volumes
3.3. Prevalence of IgG and IgM Antibodies Against EBOV in Fecal Samples of Gorillas and Chimpanzees
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Zone | Date Collected | Species | No. Tested | IgG Pos/Total Tested | IgM Pos/Total Tested |
---|---|---|---|---|---|
Lopé A | October 2009–February 2010 | Gorilla gorilla gorilla | 34 | 0/34 (0.0; 0.0–10.3) | 0/34 (0.0; 0.0–10.3) |
Pan troglodytes troglodytes | 43 | 3/43 (7; 1.4–19.0) | 3/43 (7; 1.4–19.0) | ||
Lopé B | April–November 2012 | Gorilla gorilla gorilla | 22 | 0/22 (0.0; 0.0–15.4) | 0/22 (0.0; 0.0–15.4) |
Pan troglodytes troglodytes | 79 | 1/79 (1.3; 0.0–6.8) | 5/79 (6.4; 2.0–14.1) | ||
Mwagna | July 2011 | Gorilla gorilla gorilla | 0 | 0/0 | 0/0 |
Pan troglodytes troglodytes | 5 | 0/5 | 0/5 | ||
Malouma | January–September 2012 | Gorilla gorilla gorilla | 10 | 0/10 (0.0; 0.0–30.8) | 0/10 (0.0; 30.8) |
Pan troglodytes troglodytes | 90 | 5/90 (5.5; 1.8–12.5) | 0/90 (0.0; 0.0–4.0) | ||
INP B | April 2012 | Gorilla gorilla gorilla | 32 | 10/32 (31.2; 16.1–50.0) | 3/32 (9.4; 2.0–25.0) |
Pan troglodytes troglodytes | 1 | 0/1 | 0/1 | ||
Djidi | June 2011 | Gorilla gorilla gorilla | 1 | 0/1 | 0/1 |
Pan troglodytes troglodytes | 4 | 0/4 | 0/4 | ||
INP A | July–September 2010 | Gorilla gorilla gorilla | 26 | 1/26 (3.8; 0.0–19.6) | 0/26 (0.0; 0.0–13.2) |
Pan troglodytes troglodytes | 8 | 0/8 | 0/8 |
Virus | Primers and Probes | Sequences 5′ to 3′ | References |
---|---|---|---|
EBOV | EBOZNPCONF1862 | AGCTACGGCGAATACCAGAGTT | |
EBOZNPCONR1943 | CGTCCTCGTCTAGATCGAATAGG | ||
EBOZNPCONP1885 | 6 FAM-CTCGGAAAACGGCATGAATGCACC-BHQ1 | ||
SUDV | Soudan-F | GCCATGGITTCAGGTTTGAG | [25] |
Soudan-R | GGTIACATTGGGCAACAATTCA | ||
Soudan-P | 6-FAM-ACGGTGCACATTCTCCTTTTCTCGGA-BHQ1 | ||
BDBV | Uganda-F | GAGAAAAGGCCTGTCTGGAGAA | |
Uganda-R | TCGGGTATTGAATCAGACCTTGTT | ||
Uganda-P | 6 FAM-TTCAACGACAAATCCAAGTGCACGCA-BHQ1 | ||
MARV | MBGCONTAQMF1 | GGACCACTGCTGGCCATATC | [26] |
MBGCONTAQMR3-1 | GAGAACATITCGGCAGGAAG | ||
MBG 5313 | 6 FAM-CCTAAACAGGCTTGTCTTCTCTGGGACTT-BHQ1 | ||
MBG 5313- Prb RAV | 6 FAM-ATCCTGAATAAGCTCGTCTTCTCTGGGACTT-BHQ1 | ||
Panfilo | Filo A | TATMGRAATTTTTCYTTYTCATT | [9] |
Filo B | ATGTGGTGGGYTATAAWARTCACTRACAT | ||
Filo C | GCWAAAGCMTTYCCWAGYAAYATGATGG | ||
Filo D | ATAAWARTCACTRACATGCATATAACA |
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Mombo, I.M.; Fritz, M.; Becquart, P.; Liegeois, F.; Elguero, E.; Boundenga, L.; Mebaley, T.N.; Prugnolle, F.; Maganga, G.D.; Leroy, E.M. Detection of Ebola Virus Antibodies in Fecal Samples of Great Apes in Gabon. Viruses 2020, 12, 1347. https://doi.org/10.3390/v12121347
Mombo IM, Fritz M, Becquart P, Liegeois F, Elguero E, Boundenga L, Mebaley TN, Prugnolle F, Maganga GD, Leroy EM. Detection of Ebola Virus Antibodies in Fecal Samples of Great Apes in Gabon. Viruses. 2020; 12(12):1347. https://doi.org/10.3390/v12121347
Chicago/Turabian StyleMombo, Illich M., Matthieu Fritz, Pierre Becquart, Florian Liegeois, Eric Elguero, Larson Boundenga, Telstar N. Mebaley, Franck Prugnolle, Gael D. Maganga, and Eric M. Leroy. 2020. "Detection of Ebola Virus Antibodies in Fecal Samples of Great Apes in Gabon" Viruses 12, no. 12: 1347. https://doi.org/10.3390/v12121347