Superinfection by PHYVV Alters the Recovery Process in PepGMV-Infected Pepper Plants
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material and Growth Conditions
2.2. Viral Clones
2.3. Virus Inoculation
2.4. Superinfection Assays
2.5. Viral DNA Accumulation in Infected Plants
2.6. Isolation of PepGMV Minichromosomes
2.7. Methylation Density Analysis
2.8. ChIP-qPCR Analysis
2.9. PHYVV Gene Expression Analysis
2.10. Suppressor Silencing Analysis
2.11. GFP Expression Analysis
2.12. Chop-PCR Analysis
3. Results
3.1. PHYVV Superinfection Reverts Host Recovery in PepGMV-Infected Pepper Plants
3.2. DNA Methylation Level of PepGMV Genome is Reduced in PHYVV Superinfected Plants
3.3. CHIP-qPCR Analysis
3.4. PepGMV Accumulation in Recovery Plants is Enhanced by PHYVV Encoded Rep and REn Proteins
3.5. Expression of PHYVV REn Reduces the Levels of PepGMV DNA Methylation
3.6. PHYVV REn Is a TGS Silencing Suppressor
3.7. PHYVV Rep Is a PTGS Silencing Suppressor
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Rodríguez-Gandarilla, M.G.; Rodríguez-Negrete, E.A.; Rivera-Bustamante, R.F. Superinfection by PHYVV Alters the Recovery Process in PepGMV-Infected Pepper Plants. Viruses 2020, 12, 286. https://doi.org/10.3390/v12030286
Rodríguez-Gandarilla MG, Rodríguez-Negrete EA, Rivera-Bustamante RF. Superinfection by PHYVV Alters the Recovery Process in PepGMV-Infected Pepper Plants. Viruses. 2020; 12(3):286. https://doi.org/10.3390/v12030286
Chicago/Turabian StyleRodríguez-Gandarilla, Myriam G., Edgar A. Rodríguez-Negrete, and Rafael F. Rivera-Bustamante. 2020. "Superinfection by PHYVV Alters the Recovery Process in PepGMV-Infected Pepper Plants" Viruses 12, no. 3: 286. https://doi.org/10.3390/v12030286
APA StyleRodríguez-Gandarilla, M. G., Rodríguez-Negrete, E. A., & Rivera-Bustamante, R. F. (2020). Superinfection by PHYVV Alters the Recovery Process in PepGMV-Infected Pepper Plants. Viruses, 12(3), 286. https://doi.org/10.3390/v12030286