Vector Competence of Florida Culicoides insignis (Diptera: Ceratopogonidae) for Epizootic Hemorrhagic Disease Virus Serotype-2
Abstract
:1. Introduction
2. Materials and Methods
2.1. Field Collections of Culicoides insignis
2.2. Viral Screening of Field-Collected Midges
2.3. Per Os Infections of Culicoides
2.4. Midge Tissue Collection and Processing
2.5. Statistical Analysis
2.6. Intrathoracic Inoculation Assays
3. Results
3.1. Viral Screening of Pooled Field-Collected Midges
3.2. Infection, Dissemination, and Transmission Potential of C. insignis
3.3. Infectious Titer Comparisons
3.4. Intrathoracic Inoculation Assays
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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% Overall Rates | % Adjusted Rates | |||||||
---|---|---|---|---|---|---|---|---|
Trial | Population | Titer | N | IR a | DR a | TR a | DR b | TR b |
1 | Buck Island Ranch | 5.05 | 18 | 94.4 (17) | 61.1 (11) | 27.8 (5) | 64.7 (11) | 29.4 (5) |
2 | Ocala | 4.00 | 70 | 4.3 (3) | 2.9 (2) | 0.0 (0) | 66.7 (2) | 0.0 (0) |
3 | Ocala | 2.94 | 54 | 7.4 (4) | 1.9 (1) | 1.9 (1) | 25.0 (1) | 25.0 (1) |
MI | Buck Island Ranch | 5.00 | 12 | - | - | 41.7 (5) | - | - |
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McGregor, B.L.; Erram, D.; Alto, B.W.; Lednicky, J.A.; Wisely, S.M.; Burkett-Cadena, N.D. Vector Competence of Florida Culicoides insignis (Diptera: Ceratopogonidae) for Epizootic Hemorrhagic Disease Virus Serotype-2. Viruses 2021, 13, 410. https://doi.org/10.3390/v13030410
McGregor BL, Erram D, Alto BW, Lednicky JA, Wisely SM, Burkett-Cadena ND. Vector Competence of Florida Culicoides insignis (Diptera: Ceratopogonidae) for Epizootic Hemorrhagic Disease Virus Serotype-2. Viruses. 2021; 13(3):410. https://doi.org/10.3390/v13030410
Chicago/Turabian StyleMcGregor, Bethany L., Dinesh Erram, Barry W. Alto, John A. Lednicky, Samantha M. Wisely, and Nathan D. Burkett-Cadena. 2021. "Vector Competence of Florida Culicoides insignis (Diptera: Ceratopogonidae) for Epizootic Hemorrhagic Disease Virus Serotype-2" Viruses 13, no. 3: 410. https://doi.org/10.3390/v13030410