Herpes Simplex Virus Type 2 Mucin-Like Glycoprotein mgG Promotes Virus Release from the Surface of Infected Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Cells and Viruses
2.3. Purification of HSV-2 Virions, Glycoprotein gG, and Quantification of Viral DNA
2.4. Virus Replication Kinetics and Forcible Virus Liberation Assays
2.5. Total Internal Reflection Fluorescence Microscopy
2.6. Binding of Virus to Infected Cells
2.7. Immunogold Electron Microscopy
2.8. Assay to Detect a GAG Lyase Activity
2.9. Preparation of Cellular Extracts, Electrophoresis, and Immunoblot Assay
2.10. Statistical Analysis
3. Results
3.1. Biological Activities of the mgG Deficient Mutant of HSV-2
3.2. Release of the mgG-def Virions from the Surface of Infected Cells
3.3. Interaction of mgG-def Virions with Surface-Bound CS Chains
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Trybala, E.; Peerboom, N.; Adamiak, B.; Krzyzowska, M.; Liljeqvist, J.-Å.; Bally, M.; Bergström, T. Herpes Simplex Virus Type 2 Mucin-Like Glycoprotein mgG Promotes Virus Release from the Surface of Infected Cells. Viruses 2021, 13, 887. https://doi.org/10.3390/v13050887
Trybala E, Peerboom N, Adamiak B, Krzyzowska M, Liljeqvist J-Å, Bally M, Bergström T. Herpes Simplex Virus Type 2 Mucin-Like Glycoprotein mgG Promotes Virus Release from the Surface of Infected Cells. Viruses. 2021; 13(5):887. https://doi.org/10.3390/v13050887
Chicago/Turabian StyleTrybala, Edward, Nadia Peerboom, Beata Adamiak, Malgorzata Krzyzowska, Jan-Åke Liljeqvist, Marta Bally, and Tomas Bergström. 2021. "Herpes Simplex Virus Type 2 Mucin-Like Glycoprotein mgG Promotes Virus Release from the Surface of Infected Cells" Viruses 13, no. 5: 887. https://doi.org/10.3390/v13050887