Characterization of Winter Dysentery Bovine Coronavirus Isolated from Cattle in Israel
Abstract
:1. Introduction
2. Materials and Methods
2.1. Clinical Samples
2.2. RNA Extraction and BCoV Molecular Detection
2.3. Virus Isolation
2.4. Replication of BCoV in HRT-18G
2.5. Immunofluorescence
2.6. PCR and Sequencing
2.7. Molecular Analysis of Local BCoV Strains
3. Results
3.1. Clinical Signs and Molecular Detection of BCoV
3.2. BCoV Isolation and Replication
3.3. Molecular Analysis
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Farm (#) | Isolate * | Clinical Signs | Age | Year | Ct (RT-qPCR) | Prevalence € |
---|---|---|---|---|---|---|
1 | ISR2408 | Hemorrhagic diarrhea, reduction in milk | Primipara, second and third lactation | 2017 | 20.11 | 57/700 |
ISR2391 | Hemorrhagic diarrhea, reduction in milk | Primipara, second and third lactation | 2017 | 21.92 | ||
2 | ISR1601 | Hemorrhagic diarrhea | 6 and 7 years old | 2018 | 23.5 | 1/800 |
3 | ISR1205 | Hemorrhagic diarrhea, reduction in milk | Primipara, second lactation | 2018 | 19.74 | 6/270 |
4 | ISR2382 | Hemorrhagic diarrhea, reduction in milk | Third lactation | 2018 | 20.64 | 1/70 |
5 | ISR1255 | Hemorrhagic diarrhea | 6 years old | 2019 | 25.3 | 3/190 |
6 | ISR1348 | Hemorrhagic diarrhea, reduction in milk | In lactation | 2019 | 14.75 | 6/370 |
7 | ISR2218 | Hemorrhagic diarrhea, reduction in milk | Primipara, second, third and fourth lactation | 2020 | 23.71 | 15/320 |
8 | ISR2182 | Hemorrhagic diarrhea, reduction in milk, death | Primipara, second lactation | 2020 | 16.91 | 150/300 |
9 | ISR2292 | Hemorrhagic diarrhea, reduction in milk, anorexia | Primipara, second, third and fourth lactation | 2020 | 17.02 | 7/680 |
10 | ISR2348 | Hemorrhagic diarrhea, reduction in milk | In lactation | 2020 | 20.44 | 1/350 |
11 | ISR2703 | Hemorrhagic diarrhea, reduction in milk | Primipara, second lactation | 2020 | 23.23 | 3/350 |
12 | ISR1262 | Hemorrhagic diarrhea | Primipara, second, third and fourth lactation | 2021 | 21.67 | 10/400 |
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David, D.; Storm, N.; Ilan, W.; Sol, A. Characterization of Winter Dysentery Bovine Coronavirus Isolated from Cattle in Israel. Viruses 2021, 13, 1070. https://doi.org/10.3390/v13061070
David D, Storm N, Ilan W, Sol A. Characterization of Winter Dysentery Bovine Coronavirus Isolated from Cattle in Israel. Viruses. 2021; 13(6):1070. https://doi.org/10.3390/v13061070
Chicago/Turabian StyleDavid, Dan, Nick Storm, Waksman Ilan, and Asaf Sol. 2021. "Characterization of Winter Dysentery Bovine Coronavirus Isolated from Cattle in Israel" Viruses 13, no. 6: 1070. https://doi.org/10.3390/v13061070