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Volume 14
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10.3390/v14010092
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Article

Breaking Entry-and Species Barriers: LentiBOOST® Plus Polybrene Enhances Transduction Efficacy of Dendritic Cells and Monocytes by Adenovirus 5

by
Astrid Strack
1,*,
Andrea Deinzer
1,2,
Christian Thirion
3,
Silke Schrödel
3,
Jan Dörrie
4,
Tatjana Sauerer
4,
Alexander Steinkasserer
1 and
Ilka Knippertz
1,*
1
Department of Immune Modulation, Universitätsklinikum Erlangen, Friedrich-Alexander Universität Erlangen-Nürnberg, Hartmannstr. 14, 91052 Erlangen, Germany
2
Institute of Clinical Microbiology, Immunology and Hygiene, Universitätsklinikum Erlangen, Friedrich-Alexander Universität Erlangen-Nürnberg, Wasserturmstraße 3/5, 91054 Erlangen, Germany
3
SIRION Biotech GmbH, Am Klopferspitz 19, 82152 Martinsried, Germany
4
Department of Dermatology, Universitätsklinikum Erlangen, Friedrich-Alexander Universität Erlangen-Nürnberg, Hartmannstr. 14, 91052 Erlangen, Germany
*
Authors to whom correspondence should be addressed.
Viruses 2022, 14(1), 92; https://doi.org/10.3390/v14010092
Submission received: 20 December 2021 / Accepted: 29 December 2021 / Published: 5 January 2022
(This article belongs to the Special Issue Novel Developments and Perspectives in Viral Vector Technology)
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Abstract

Due to their ability to trigger strong immune responses, adenoviruses (HAdVs) in general and the serotype5 (HAdV-5) in particular are amongst the most popular viral vectors in research and clinical application. However, efficient transduction using HAdV-5 is predominantly achieved in coxsackie and adenovirus receptor (CAR)-positive cells. In the present study, we used the transduction enhancer LentiBOOST® comprising the polycationic Polybrene to overcome these limitations. Using LentiBOOST®/Polybrene, we yielded transduction rates higher than 50% in murine bone marrow-derived dendritic cells (BMDCs), while maintaining their cytokine expression profile and their capability to induce T-cell proliferation. In human dendritic cells (DCs), we increased the transduction rate from 22% in immature (i)DCs or 43% in mature (m)DCs to more than 80%, without inducing cytotoxicity. While expression of specific maturation markers was slightly upregulated using LentiBOOST®/Polybrene on iDCs, no effect on mDC phenotype or function was observed. Moreover, we achieved efficient HAdV5 transduction also in human monocytes and were able to subsequently differentiate them into proper iDCs and functional mDCs. In summary, we introduce LentiBOOST® comprising Polybrene as a highly potent adenoviral transduction agent for new in-vitro applications in a set of different immune cells in both mice and humans.
Keywords: adenovirus; adenoviral transduction; monocytes; dendritic cells; T cells; LentiBOOST® adenovirus; adenoviral transduction; monocytes; dendritic cells; T cells; LentiBOOST®

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MDPI and ACS Style

Strack, A.; Deinzer, A.; Thirion, C.; Schrödel, S.; Dörrie, J.; Sauerer, T.; Steinkasserer, A.; Knippertz, I. Breaking Entry-and Species Barriers: LentiBOOST® Plus Polybrene Enhances Transduction Efficacy of Dendritic Cells and Monocytes by Adenovirus 5. Viruses 2022, 14, 92. https://doi.org/10.3390/v14010092

AMA Style

Strack A, Deinzer A, Thirion C, Schrödel S, Dörrie J, Sauerer T, Steinkasserer A, Knippertz I. Breaking Entry-and Species Barriers: LentiBOOST® Plus Polybrene Enhances Transduction Efficacy of Dendritic Cells and Monocytes by Adenovirus 5. Viruses. 2022; 14(1):92. https://doi.org/10.3390/v14010092

Chicago/Turabian Style

Strack, Astrid, Andrea Deinzer, Christian Thirion, Silke Schrödel, Jan Dörrie, Tatjana Sauerer, Alexander Steinkasserer, and Ilka Knippertz. 2022. "Breaking Entry-and Species Barriers: LentiBOOST® Plus Polybrene Enhances Transduction Efficacy of Dendritic Cells and Monocytes by Adenovirus 5" Viruses 14, no. 1: 92. https://doi.org/10.3390/v14010092

APA Style

Strack, A., Deinzer, A., Thirion, C., Schrödel, S., Dörrie, J., Sauerer, T., Steinkasserer, A., & Knippertz, I. (2022). Breaking Entry-and Species Barriers: LentiBOOST® Plus Polybrene Enhances Transduction Efficacy of Dendritic Cells and Monocytes by Adenovirus 5. Viruses, 14(1), 92. https://doi.org/10.3390/v14010092

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