Increased CD4+CD8+ Double Positive T Cells during Hantaan Virus Infection
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Cohort and PBMC Isolation
2.2. Single Cell RNA Sequencing
2.3. Flow Cytometry
2.4. In Vitro Infection
2.5. Statistical Analysis
3. Results
3.1. DP T Cells Increased in HFRS Patients
3.2. The Gene Expression Profile of DP T Cells
3.3. DP T Cells Activated and Played Anti-Viral Role in HFRS Patients
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Mild/Moderate | Severe/Critical | NC | |
---|---|---|---|
Demographic characteristics | |||
number | 21 | 38 | 37 |
Age (years) | 43 (17–74) | 45 (20–70) | 36 (21–55) |
Male (%) | 82.6% | 89.1% | 70.0% |
Sample number | 23 | 43 | |
Acute phase (febrile/hypotensive/oliguric) | 11 | 24 | _ |
Convalescent phase (diuretic/convalescent) | 12 | 19 | _ |
Sample No. | Age | Sex | Disease Phase | Disease Severity | DP T Cell Counts/Total Sequencing Cell Counts |
---|---|---|---|---|---|
P04 | 52 | male | oligouria | critical | 28/4556 |
P06 | 34 | male | oligouria | moderate | 36/3746 |
P07 | 49 | male | fever | moderate | 189/4218 |
P15 | 29 | male | fever, shock, and oligouria | critical | 68/4551 |
P16 | 37 | male | fever | moderate | 23/4129 |
P26 | 26 | male | fever, and shock | critical | 66/3763 |
NC1 | 29 | male | / | / | 9/4253 |
NC2 | 33 | female | / | / | 23/3660 |
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Zhang, H.; Wang, Y.; Ma, Y.; Tang, K.; Zhang, C.; Wang, M.; Zhang, X.; Xue, M.; Jia, X.; Hu, H.; et al. Increased CD4+CD8+ Double Positive T Cells during Hantaan Virus Infection. Viruses 2022, 14, 2243. https://doi.org/10.3390/v14102243
Zhang H, Wang Y, Ma Y, Tang K, Zhang C, Wang M, Zhang X, Xue M, Jia X, Hu H, et al. Increased CD4+CD8+ Double Positive T Cells during Hantaan Virus Infection. Viruses. 2022; 14(10):2243. https://doi.org/10.3390/v14102243
Chicago/Turabian StyleZhang, Huiyuan, Yazhen Wang, Ying Ma, Kang Tang, Chunmei Zhang, Meng Wang, Xiyue Zhang, Manling Xue, Xiaozhou Jia, Haifeng Hu, and et al. 2022. "Increased CD4+CD8+ Double Positive T Cells during Hantaan Virus Infection" Viruses 14, no. 10: 2243. https://doi.org/10.3390/v14102243