Characterization of a Novel Mycovirus from the Phytopathogenic Fungus Botryosphaeria dothidea

Wang, Yanfen; Zhao, Hang; Cao, Jiayuan; Yin, Xinming; Guo, Yashuang; Guo, Lihua; Wu, Haiyan; Zhang, Meng

doi:10.3390/v14020331

Open AccessCommunication

Characterization of a Novel Mycovirus from the Phytopathogenic Fungus Botryosphaeria dothidea

by

Yanfen Wang

^1,†

,

Hang Zhao

^1,†,

Jiayuan Cao

¹,

Xinming Yin

¹,

Yashuang Guo

¹,

Lihua Guo

²,

Haiyan Wu

^3,* and

Meng Zhang

^1,2,*

¹

School of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China

²

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China

³

Analytical Instrument Center, Henan Agricultural University, Zhengzhou 450002, China

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this study.

Viruses 2022, 14(2), 331; https://doi.org/10.3390/v14020331

Submission received: 15 December 2021 / Revised: 1 February 2022 / Accepted: 2 February 2022 / Published: 6 February 2022

(This article belongs to the Special Issue Diversity and Coinfections of Plant or Fungal Viruses)

Download

Browse Figures

Versions Notes

Abstract

Botryosphaeria dothidea is, globally, one of the most economically important phytopathogenic fungi worldwide, causing the canker and dieback of fruit trees. An increasing number of viruses infecting B. dothidea have lately been reported, several of which could confer hypovirulence. In this study, isolated from strain ZM170285-1 of B. dothidea, a novel double-stranded RNA (dsRNA) mycovirus, tentatively named Botryosphaeria dothidea partitivirus 2 (BdPV2), was identified well. The BdPV2 harbored three dsRNA segments (1–3) with lengths of 1751, 1568, and 1198 bp, which encoded an RNA-dependent RNA polymerase (RdRp), a capsid protein (CP), and a hypothetical protein of unknown function, respectively. BLASTp searches revealed that the predicted protein sequences of dsRNA1 and dsRNA2 had the highest identities (74.95% and 61.01%) with the corresponding dsRNAs of Penicillium stoloniferum virus S (PsV-S), whereas dsRNA3 shared the highest identity (32.95%) with the dsRNA3 of Aspergillus ochraceous virus 1 (AoV1). Phylogenetic analysis indicated that BdPV2 belonged to the Gammapartitivirus genus and Partitiviridae family. To our knowledge, this is the first report of a Gammapartitivirus in B. dothidea.

Keywords: Botryosphaeria dothidea; Botryosphaeria dothidea partitivirus 2 (BdPV2); Gammapartitivirus

Share and Cite

MDPI and ACS Style

Wang, Y.; Zhao, H.; Cao, J.; Yin, X.; Guo, Y.; Guo, L.; Wu, H.; Zhang, M. Characterization of a Novel Mycovirus from the Phytopathogenic Fungus Botryosphaeria dothidea. Viruses 2022, 14, 331. https://doi.org/10.3390/v14020331

AMA Style

Wang Y, Zhao H, Cao J, Yin X, Guo Y, Guo L, Wu H, Zhang M. Characterization of a Novel Mycovirus from the Phytopathogenic Fungus Botryosphaeria dothidea. Viruses. 2022; 14(2):331. https://doi.org/10.3390/v14020331

Chicago/Turabian Style

Wang, Yanfen, Hang Zhao, Jiayuan Cao, Xinming Yin, Yashuang Guo, Lihua Guo, Haiyan Wu, and Meng Zhang. 2022. "Characterization of a Novel Mycovirus from the Phytopathogenic Fungus Botryosphaeria dothidea" Viruses 14, no. 2: 331. https://doi.org/10.3390/v14020331

APA Style

Wang, Y., Zhao, H., Cao, J., Yin, X., Guo, Y., Guo, L., Wu, H., & Zhang, M. (2022). Characterization of a Novel Mycovirus from the Phytopathogenic Fungus Botryosphaeria dothidea. Viruses, 14(2), 331. https://doi.org/10.3390/v14020331

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Menu

Characterization of a Novel Mycovirus from the Phytopathogenic Fungus Botryosphaeria dothidea

Abstract

Share and Cite

Article Metrics

Article Access Statistics

Further Information

Guidelines

MDPI Initiatives

Follow MDPI