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Volume 15
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Correction to Viruses 2023, 15(4), 965.
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Correction

Correction: Alathari et al. A Multiplexed, Tiled PCR Method for Rapid Whole-Genome Sequencing of Infectious Spleen and Kidney Necrosis Virus (ISKNV) in Tilapia. Viruses 2023, 15, 965

by
Shayma Alathari
1,*,
Dominique L. Chaput
1,
Luis M. Bolaños
1,
Andrew Joseph
2,
Victoria L. N. Jackson
1,
David Verner-Jeffreys
2,3,
Richard Paley
2,
Charles R. Tyler
1,3 and
Ben Temperton
1,*
1
Department of Biosciences, University of Exeter, Stocker Road, Exeter EX4 4QD, UK
2
Centre for Environment, Fisheries and Aquaculture Science (Cefas), The Nothe, Barrack Road, Weymouth DT4 8UB, UK
3
Sustainable Aquaculture Futures Centre, University of Exeter, Exeter EX4 4QD, UK
*
Authors to whom correspondence should be addressed.
Viruses 2023, 15(7), 1476; https://doi.org/10.3390/v15071476
Submission received: 7 June 2023 / Accepted: 8 June 2023 / Published: 29 June 2023
(This article belongs to the Section Animal Viruses)
Browse Figures
Versions Notes
In the original publication [1], there was a mistake in Figure 1 and Figure 2 as published.
In Figure 1, there is a loss of colour in most of the dots representing the farms on the map. The corrected Figure 1 appears below.
In Figure 2, there is a loss of all dots. The corrected Figure 2 appears below.
The authors state that the scientific conclusions are unaffected. This correction was approved by the Academic Editor. The original publication has also been updated.

Reference

  1. Alathari, S.; Chaput, D.L.; Bolaños, L.M.; Joseph, A.; Jackson, V.L.N.; Verner-Jeffreys, D.; Paley, R.; Tyler, C.R.; Temperton, B. A Multiplexed, Tiled PCR Method for Rapid Whole-Genome Sequencing of Infectious Spleen and Kidney Necrosis Virus (ISKNV) in Tilapia. Viruses 2023, 15, 965. [Google Scholar] [CrossRef] [PubMed]
Viruses 15 01476 g001 550
Figure 1. A map of the lower region of Lake Volta in Ghana, West Africa, showing the date and location of the farms where the outbreaks of mortality occurred; locations retrieved from [11]. This map was constructed using ArcGIS (GIS software). Version 10.0. Redlands, CA, USA: Environmental Systems Research Institute, Inc., 2010.
Figure 1. A map of the lower region of Lake Volta in Ghana, West Africa, showing the date and location of the farms where the outbreaks of mortality occurred; locations retrieved from [11]. This map was constructed using ArcGIS (GIS software). Version 10.0. Redlands, CA, USA: Environmental Systems Research Institute, Inc., 2010.
Viruses 15 01476 g001
Viruses 15 01476 g002 550
Figure 2. Successful recovery of >50% of the ISKNV genome required 482 template strands per µL (2410 viral templates per 5 µL sequencing reaction), with a minimum of 0.2 copies per µL to recover >0% of the genome with at least 20-fold coverage for error correction. Number of viral templates was measured using ddPCR from a serially diluted ISKNV template, which was subsequently sequenced and processed as described in the text.
Figure 2. Successful recovery of >50% of the ISKNV genome required 482 template strands per µL (2410 viral templates per 5 µL sequencing reaction), with a minimum of 0.2 copies per µL to recover >0% of the genome with at least 20-fold coverage for error correction. Number of viral templates was measured using ddPCR from a serially diluted ISKNV template, which was subsequently sequenced and processed as described in the text.
Viruses 15 01476 g002
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MDPI and ACS Style

Alathari, S.; Chaput, D.L.; Bolaños, L.M.; Joseph, A.; Jackson, V.L.N.; Verner-Jeffreys, D.; Paley, R.; Tyler, C.R.; Temperton, B. Correction: Alathari et al. A Multiplexed, Tiled PCR Method for Rapid Whole-Genome Sequencing of Infectious Spleen and Kidney Necrosis Virus (ISKNV) in Tilapia. Viruses 2023, 15, 965. Viruses 2023, 15, 1476. https://doi.org/10.3390/v15071476

AMA Style

Alathari S, Chaput DL, Bolaños LM, Joseph A, Jackson VLN, Verner-Jeffreys D, Paley R, Tyler CR, Temperton B. Correction: Alathari et al. A Multiplexed, Tiled PCR Method for Rapid Whole-Genome Sequencing of Infectious Spleen and Kidney Necrosis Virus (ISKNV) in Tilapia. Viruses 2023, 15, 965. Viruses. 2023; 15(7):1476. https://doi.org/10.3390/v15071476

Chicago/Turabian Style

Alathari, Shayma, Dominique L. Chaput, Luis M. Bolaños, Andrew Joseph, Victoria L. N. Jackson, David Verner-Jeffreys, Richard Paley, Charles R. Tyler, and Ben Temperton. 2023. "Correction: Alathari et al. A Multiplexed, Tiled PCR Method for Rapid Whole-Genome Sequencing of Infectious Spleen and Kidney Necrosis Virus (ISKNV) in Tilapia. Viruses 2023, 15, 965" Viruses 15, no. 7: 1476. https://doi.org/10.3390/v15071476

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