Four Novel Caudoviricetes Bacteriophages Isolated from Baltic Sea Water Infect Colonizers of Aurelia aurita
Abstract
:1. Introduction
2. Material and Methods
2.1. Bacterial Strains and Growth Conditions
2.2. Taxonomic Classification of Bacterial Isolates
2.3. Phage Enrichment, Isolation, and Purification
2.4. Phage Purification, Titration, and Propagation
2.5. Transmission Electron Microscopy
2.6. One-Step Growth Curve Analysis
2.7. Efficiency of Plating and Host Range
2.8. Viral DNA Isolation
2.9. Sequencing, Bioinformatic Analysis, and Annotation of Viral Genomes
3. Results
3.1. Isolation of Bacteriophages from Baltic Seawater Targeting Marine Bacteria Associated with A. aurita
3.2. Plaque and Virion Morphology Assign the Phages to the Class of Caudoviricetes
3.3. Phages KMM1, KMM2, and KMM4 Have a Shorter Lytic Cycle Than Phage KMM3
3.4. KMM1 Is a Powerful Broad-Host-Range Phage, Whereas KMM2, KMM3, and KMM4 Are Narrow-Host-Range Phages
3.5. Novel Phage Species Confirmed by Genome Sequencing Analysis
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Strain No. | Strain | Reference | Phylum | Class | Order | Family | Source | Growth Medium | Growth Temp. | Use in This Study |
---|---|---|---|---|---|---|---|---|---|---|
74 | Micrococcus luteus | MK967048.1 | Actinomycetota | Actinomycetia | Micrococcales | Micrococcaceae | A. aurita polyp Baltic Sea husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
75 | Arthrobacter sp. | MK967049.1 | Actinomycetota | Actinomycetia | Micrococcales | Micrococcaceae | A. aurita polyp Baltic Sea husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
83 | Gordonia terrae | MK967057.1 | Actinomycetota | Actinomycetia | Mycobacteriales | Gordoniaceae | A. aurita polyp Baltic Sea husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
15 | Sulfitobacter sp. | MK967015.1 | Pseudomonadota | Alphaproteobacteria | Rhodobacterales | Rhodobacteraceae | A. aurita medusa Baltic Sea | Marine Bouillon | 30 °C | enrichment/ first screening |
20 | Sulfitobacter pontiacus | MK967020.1 | Pseudomonadota | Alphaproteobacteria | Rhodobacterales | Rhodobacteraceae | A. aurita medusa Baltic Sea husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
23 | Sulfitobacter sp. | MK967023.1 | Pseudomonadota | Alphaproteobacteria | Rhodobacterales | Rhodobacteraceae | A. aurita medusa Baltic Sea husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
69 | Rhodobacter sp. | MK967043.1 | Pseudomonadota | Alphaproteobacteria | Rhodobacterales | Rhodobacteraceae | M. leidyi Baltic Sea husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
78 | Sulfitobacter sp. | MK967052.1 | Pseudomonadota | Alphaproteobacteria | Rhodobacterales | Rhodobacteraceae | A. aurita polyp Baltic Sea husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
86 | Ruegeria sp. | MK967060.1 | Pseudomonadota | Alphaproteobacteria | Rhodobacterales | Rhodobacteraceae | A. aurita polyp Baltic Sea husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
89 | Ruegeria sp. | MK967063.1 | Pseudomonadota | Alphaproteobacteria | Rhodobacterales | Rhodobacteraceae | A. aurita polyp Baltic Sea husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
100 | Sulfitobacter sp. | MK967074.1 | Pseudomonadota | Alphaproteobacteria | Rhodobacterales | Rhodobacteraceae | A. aurita polyp North Sea husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
117 | Ruegeria mobilis | MK967091.1 | Pseudomonadota | Alphaproteobacteria | Rhodobacterales | Rhodobacteraceae | A. aurita polyp North Atlantic husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
147 | Phaeobacter gallaeciensis | MK967120.1 | Pseudomonadota | Alphaproteobacteria | Rhodobacterales | Rhodobacteraceae | Artificial Seawater 18 PSU | Marine Bouillon | 30 °C | enrichment/ first screening |
188 | Sulfitobacter pseudonitzschiae | MK967160.1 | Pseudomonadota | Alphaproteobacteria | Rhodobacterales | Rhodobacteraceae | Artificial Seawater 30 PSU | Marine Bouillon | 30 °C | enrichment/ first screening |
13 | Bacillus cereus | MK967013.1 | Bacillota | Bacilli | Bacillales | Bacillaceae | A. aurita medusa Baltic Sea | Marine Bouillon | 30 °C | enrichment/ first screening |
16 | Bacillus sp. | MK967016.1 | Bacillota | Bacilli | Bacillales | Bacillaceae | A. aurita medusa Baltic Sea | Marine Bouillon | 30 °C | enrichment/ first screening |
17 | Bacillus cereus | MK967017.1 | Bacillota | Bacilli | Bacillales | Bacillaceae | A. aurita medusa Baltic Sea | Marine Bouillon | 30 °C | enrichment/ first screening |
19 | Bacillus sp. | MK967019.1 | Bacillota | Bacilli | Bacillales | Bacillaceae | A. aurita medusa Baltic Sea husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
76 | Bacillus weihenstephanensis | MK967050.1 | Bacillota | Bacilli | Bacillales | Bacillaceae | A. aurita polyp Baltic Sea husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
85 | Staphylococcus warneri | MK967059.1 | Bacillota | Bacilli | Bacillales | Staphylococcaceae | A. aurita polyp Baltic Sea husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
88 | Staphylococcus sp. | MK967062.1 | Bacillota | Bacilli | Bacillales | Staphylococcaceae | A. aurita polyp Baltic Sea husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
73 | Enterococcus casseliflavus | MK967047.1 | Bacillota | Bacilli | Lactobacillales | Enterococcaceae | A. aurita polyp Baltic Sea husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
24 | Maribacter sp. | MK967024.1 | Bacteroidota | Flavobacteriia | Flavobacteriales | Flavobacteriaceae | A. aurita medusa Baltic Sea husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
57 | Olleya marilimosa | MK967032.1 | Bacteroidota | Flavobacteriia | Flavobacteriales | Flavobacteriaceae | M. leidyi Baltic Sea | Marine Bouillon | 30 °C | enrichment/ first screening |
79 | Olleya sp. | MK967053.1 | Bacteroidota | Flavobacteriia | Flavobacteriales | Flavobacteriaceae | A. aurita polyp Baltic Sea husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
181 | Chryseobacterium sp. | MK967154.1 | Bacteroidota | Flavobacteriia | Flavobacteriales | Weeksellaceae | Artificial Seawater 30 PSU | Marine Bouillon | 30 °C | enrichment/ first screening |
257 | Chryseobacterium sp. | MK967218.1 | Bacteroidota | Flavobacteriia | Flavobacteriales | Weeksellaceae | M. leidyi Baltic Sea husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
22 | Pseudolateromonas sp. | MK967022.1 | Pseudomonadota | Gammaproteobacteria | Alteromonadales | Pseudoalteromonadaceae | A. aurita medusa Baltic Sea husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
91 | Pseudoalteromonas prydzensis | MK967065.1 | Pseudomonadota | Gammaproteobacteria | Alteromonadales | Pseudoalteromonadaceae | A. aurita polyp Baltic Sea husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
101 | Pseudoalteromonas issachenkonii | MK967075.1 | Pseudomonadota | Gammaproteobacteria | Alteromonadales | Pseudoalteromonadaceae | A. aurita polyp North Sea husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
167 | Pseudoalteromonas sp. | MK967140.1 | Pseudomonadota | Gammaproteobacteria | Alteromonadales | Pseudoalteromonadaceae | Artificial Seawater 18 PSU | Marine Bouillon | 30 °C | enrichment/ first screening |
203 | Pseudoalteromonas espejiana | MK967174.1 | Pseudomonadota | Gammaproteobacteria | Alteromonadales | Pseudoalteromonadaceae | Artificial Seawater 30 PSU | Marine Bouillon | 30 °C | enrichment/ first screening |
219 | Pseudoalteromonas tunicata | MK967188.1 | Pseudomonadota | Gammaproteobacteria | Alteromonadales | Pseudoalteromonadaceae | M. leidyi Baltic Sea | Marine Bouillon | 30 °C | enrichment/ first screening |
224 | Pseudoalteromonas lipolytica | MK967191.1 | Pseudomonadota | Gammaproteobacteria | Alteromonadales | Pseudoalteromonadaceae | M. leidyi Baltic Sea | Marine Bouillon | 30 °C | enrichment/ first screening |
105 | Shewanella basaltis | MK967079.1 | Pseudomonadota | Gammaproteobacteria | Alteromonadales | Shewanellaceae | A. aurita polyp North Sea husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
21 | Cobetia amphilecti | MK967021.1 | Pseudomonadota | Gammaproteobacteria | Oceanospirillales | Halomonadaceae | A. aurita medusa Baltic Sea husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
55 | Marinomonas hwangdonensis | MK967030.1 | Pseudomonadota | Gammaproteobacteria | Oceanospirillales | Oceanospirillaceae | M. leidyi Baltic Sea | Marine Bouillon | 30 °C | enrichment/ first screening |
222 | Marinomonas pontica | MK967189.1 | Pseudomonadota | Gammaproteobacteria | Oceanospirillales | Oceanospirillaceae | M. leidyi Baltic Sea | Marine Bouillon | 30 °C | enrichment/ first screening |
262 | Oceanospirillaceae bacterium | MK967222.1 | Pseudomonadota | Gammaproteobacteria | Oceanospirillales | Oceanospirillaceae | M. leidyi Baltic Sea | Marine Bouillon | 30 °C | enrichment/ first screening |
11 | Pseudomonas sp. | MK967012.1 | Pseudomonadota | Gammaproteobacteria | Pseudomonadales | Pseudomonadaceae | A. aurita medusa Baltic Sea | Marine Bouillon | 30 °C | enrichment/ first screening |
90 | Pseudomonas putida | MK967064.1 | Pseudomonadota | Gammaproteobacteria | Pseudomonadales | Pseudomonadaceae | A. aurita polyp Baltic Sea husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
92 | Pseudomonas putida | MK967066.1 | Pseudomonadota | Gammaproteobacteria | Pseudomonadales | Pseudomonadaceae | A. aurita polyp Baltic Sea husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
93 | Pseudomonas sp. | MK967067.1 | Pseudomonadota | Gammaproteobacteria | Pseudomonadales | Pseudomonadaceae | A. aurita polyp Baltic Sea husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
94 | Pseudomonas sp. | MK967068.1 | Pseudomonadota | Gammaproteobacteria | Pseudomonadales | Pseudomonadaceae | A. aurita polyp Baltic Sea husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
132 | Pseudomonas fluorescens | MK967106.1 | Pseudomonadota | Gammaproteobacteria | Pseudomonadales | Pseudomonadaceae | Artificial Seawater 18 PSU | Marine Bouillon | 30 °C | enrichment/ first screening |
196 | Pseudomonas syringae | MK967168.1 | Pseudomonadota | Gammaproteobacteria | Pseudomonadales | Pseudomonadaceae | Artificial Seawater 30 PSU | Marine Bouillon | 30 °C | enrichment/ first screening |
77 | Vibrio anguillarum | MK967051.1 | Pseudomonadota | Gammaproteobacteria | Vibrionales | Vibrionaceae | A. aurita polyp Baltic Sea husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
80 | Vibrio anguillarum | MK967054.1 | Pseudomonadota | Gammaproteobacteria | Vibrionales | Vibrionaceae | A. aurita polyp Baltic Sea husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
18 | Staphylococcus aureus | OQ398157 | Bacillota | Bacilli | Bacillales | Staphylococcaceae | A. aurita medusa Baltic Sea husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
134 | Staphylococcus aureus | OQ398164 | Bacillota | Bacilli | Bacillales | Staphylococcaceae | Artificial Seawater 18 PSU | Marine Bouillon | 30 °C | enrichment/ first screening |
87 | Staphylococcus aureus | OQ398160 | Bacillota | Bacilli | Bacillales | Staphylococcaceae | A. aurita polyp Baltic Sea husbandry | Marine Bouillon | 30 °C | enrichment/ first screening |
14 | Staphylococcus warneri | OQ398156 | Bacillota | Bacilli | Bacillales | Staphylococcaceae | A. aurita medusa Baltic Sea | Marine Bouillon | 30 °C | enrichment/ first screening |
6 | Citrobacter freundii | OQ398153 | Pseudomonadota | Gammaproteobacteria | Enterobacterales | Enterobacteriaceae | A. aurita medusa Baltic Sea | Marine Bouillon | 30 °C | enrichment/ first screening/ host range |
7 | Citrobacter sp. | OQ398154 | Pseudomonadota | Gammaproteobacteria | Enterobacterales | Enterobacteriaceae | A. aurita medusa Baltic Sea | Marine Bouillon | 30 °C | enrichment/ first screening/ host range |
8 | Pseudomonas sp. | MK967010.1 | Pseudomonadota | Gammaproteobacteria | Pseudomonadales | Pseudomonadaceae | A. aurita medusa Baltic Sea | Marine Bouillon | 30 °C | enrichment/ first screening/ host range |
62 | Sulfitobacter pontiacus | OQ398158 | Pseudomonadota | Alphaproteobacteria | Rhodobacterales | Rhodobacteraceae | M. leidyi Baltic Sea | Marine Bouillon | 30 °C | host range |
97 | Shewanella sp. | OQ398161 | Pseudomonadota | Gammaproteobacteria | Alteromonadales | Shewanellaceae | A. aurita polyp North Sea husbandry | Marine Bouillon | 30 °C | host range |
199 | Staphylococcus aureus | OQ398168 | Bacillota | Bacilli | Bacillales | Staphylococcaceae | Artificial Seawater 30 PSU | Marine Bouillon | 30 °C | host range |
DSMZ 11823 | Staphylococcus aureus | DSMZ 11823 | Bacillota | Bacilli | Bacillales | Staphylococcaceae | clinical material | Trypticase Soy Yeast Broth | 37 °C | host range |
67 | Staphylococcus aureus | OQ398159 | Bacillota | Bacilli | Bacillales | Staphylococcaceae | M. leidyi Baltic Sea husbandry | Marine Bouillon | 30 °C | host range |
102 | Staphylococcus aureus | OQ398162 | Bacillota | Bacilli | Bacillales | Staphylococcaceae | A. aurita polyp North Sea husbandry | Marine Bouillon | 30 °C | host range |
158 | Staphylococcus aureus | OQ398165 | Bacillota | Bacilli | Bacillales | Staphylococcaceae | Artificial Seawater 18 PSU | Marine Bouillon | 30 °C | host range |
161 | Staphylococcus aureus | OQ398166 | Bacillota | Bacilli | Bacillales | Staphylococcaceae | Artificial Seawater 18 PSU | Marine Bouillon | 30 °C | host range |
127 | Staphylococcus aureus | OQ398163 | Bacillota | Bacilli | Bacillales | Staphylococcaceae | A. aurita polyp North Atlantic husbandry | Marine Bouillon | 30 °C | host range |
DSMZ 28319 | Staphylococcus epidermidis | DSMZ 28319 | Bacillota | Bacilli | Bacillales | Staphylococcaceae | catheter sepsis | Trypticase Soy Yeast Broth | 37 °C | host range |
DSMZ 20328 | Staphylococcus hominis | DSMZ 20328 | Bacillota | Bacilli | Bacillales | Staphylococcaceae | human skin | Trypticase Soy Yeast Broth | 37 °C | host range |
DSMZ 100616 | Staphylococcus warneri | DSMZ 100616 | Bacillota | Bacilli | Bacillales | Staphylococcaceae | cleanroom facility, TAS | Trypticase Soy Yeast Broth | 30 °C | host range |
DSMZ 12643 | Streptococcus mitis | DSMZ 12643 | Bacillota | Bacilli | Lactobacillales | Streptococcaceae | oral cavity, human | Trypticase Soy Yeast Broth | 37 °C | host range |
DSMZ 20523 | Streptococcus mutans | DSMZ 20523 | Bacillota | Bacilli | Lactobacillales | Streptococcaceae | carious dentine | Trypticase Soy Yeast Broth | 37 °C | host range |
296 | Citrobacter braakii | OQ398170 | Pseudomonadota | Gammaproteobacteria | Enterobacterales | Enterobacteriaceae | A. aurita polyp North Atlantic husbandry | Marine Bouillon | 30 °C | host range |
283 | Citrobacter freundii | OQ398169 | Pseudomonadota | Gammaproteobacteria | Enterobacterales | Enterobacteriaceae | A. aurita polyp Baltic Sea husbandry | Marine Bouillon | 30 °C | host range |
321 | Citrobacter freundii | OQ398172 | Pseudomonadota | Gammaproteobacteria | Enterobacterales | Enterobacteriaceae | Artifical Seawater 30 PSU | Marine Bouillon | 30 °C | host range |
313 | Citrobacter sp. | OQ398171 | Pseudomonadota | Gammaproteobacteria | Enterobacterales | Enterobacteriaceae | Artifical Seawater 18 PSU | Marine Bouillon | 30 °C | host range |
DSMZ 18039 | Escherichia coli | DSMZ 18039 | Pseudomonadota | Gammaproteobacteria | Enterobacterales | Enterobacteriaceae | unknown source | Luria-Bertani Bouillon | 37 °C | host range |
strain 8 | Escherichia coli | [47] | Pseudomonadota | Gammaproteobacteria | Enterobacterales | Enterobacteriaceae | unknown source | Luria-Bertani Bouillon | 37 °C | host range |
DSMZ 30083 | Escherichia coli | DSMZ 30083 | Pseudomonadota | Gammaproteobacteria | Enterobacterales | Enterobacteriaceae | urine | Luria-Bertani Bouillon | 37 °C | host range |
DSMZ 13698 | Escherichia fergusonii | DSMZ 13698 | Pseudomonadota | Gammaproteobacteria | Enterobacterales | Enterobacteriaceae | faeces of 1-year-old boy | Luria-Bertani Bouillon | 37 °C | host range |
strain 27 | Klebsiella oxytoca | Prof. Dr. Podschun, (National Reference Laboratory for Klebsiella species, Kiel University) | Pseudomonadota | Gammaproteobacteria | Enterobacterales | Enterobacteriaceae | unknown source | Nutrient Broth | 30 °C | host range |
DSMZ 30104 | Klebsiella pneumoniae | DSMZ 30104 | Pseudomonadota | Gammaproteobacteria | Enterobacterales | Enterobacteriaceae | unknown source | Nutrient Broth | 30 °C | host range |
DSMZ 4782 | Shigella flexeneri | DSMZ 4782 | Pseudomonadota | Gammaproteobacteria | Enterobacterales | Enterobacteriaceae | unknown source | Caso Bouillon | 37 °C | host range |
DSMZ 1707 | Pseudomonas aeruginosa | DSMZ 1707 | Pseudomonadota | Gammaproteobacteria | Pseudomonadales | Pseudomonadaceae | unknown source | Caso Bouillon | 30 °C | host range |
9 | Pseudomonas sp. | OQ398155 | Pseudomonadota | Gammaproteobacteria | Pseudomonadales | Pseudomonadaceae | A. aurita medusa Baltic Sea | Marine Bouillon | 30 °C | host range |
170 | Pseudomonas sp. | OQ398167 | Pseudomonadota | Gammaproteobacteria | Pseudomonadales | Pseudomonadaceae | Artificial Seawater 18 PSU | Marine Bouillon | 30 °C | host range |
DSMZ 50256 | Pseudomonas syringae | DSMZ 50256 | Pseudomonadota | Gammaproteobacteria | Pseudomonadales | Pseudomonadaceae | Triticum aestivum, glume rot of wheat | Caso Bouillon | 30 °C | host range |
24 | Maribacter sp. | MK967024.1 | Bacteroidota | Flavobacteriia | Flavobacteriales | Flavobacteriaceae | A. aurita medusa Baltic Sea husbandry | Marine Bouillon | 30 °C | host range |
79 | Olleya sp. | MK967053.1 | Bacteroidota | Flavobacteriia | Flavobacteriales | Flavobacteriaceae | A. aurita polyp Baltic Sea husbandry | Marine Bouillon | 30 °C | host range |
98 | Olleya marilimosa | MK967072.1 | Bacteroidota | Flavobacteriia | Flavobacteriales | Flavobacteriaceae | A. aurita polyp North Sea husbandry | Marine Bouillon | 30 °C | host range |
108 | Chryseobacterium hominis | MK967082.1 | Bacteroidota | Flavobacteriia | Flavobacteriales | Flavobacteriaceae | A. aurita polyp North Sea husbandry | Marine Bouillon | 30 °C | host range |
57 | Olleya marilimosa | MK967032.1 | Bacteroidota | Flavobacteriia | Flavobacteriales | Flavobacteriaceae | M. leidyi Baltic Sea | Marine Bouillon | 30 °C | host range |
199 | Maribacter sp. | MK967170.1 | Bacteroidota | Flavobacteriia | Flavobacteriales | Flavobacteriaceae | Artificial Seawater 30 PSU | Marine Bouillon | 30 °C | host range |
Phage | NCBI Accession No. | No. of Reads | No. of Filtered Reads | Sequence Coverage | N50 | Genome Length (bps) | GC Content (%) | Predicted ORFs | Unknown Proteins |
---|---|---|---|---|---|---|---|---|---|
Pseudomonas phage BSwM KMM1 | OP902294 | 4.085 | 3.214 | 247.488 | 17.553 | 137.386 | 31.77 | 259 | 200 |
Citrobacter phage BSwM KMM2 | OP902295 | 810 | 595 | 74.163 | 22.118 | 88.537 | 39.55 | 137 | 94 |
Citrobacter phage BSwS KMM3 | OP902292 | 837 | 598 | 130.676 | 20.517 | 49.164 | 43.17 | 92 | 58 |
Citrobacter phage BSwM KMM4 | OP902293 | 6.433 | 5.371 | 544.327 | 23.894 | 86.911 | 39.02 | 138 | 100 |
KMM1 | KMM2 | KMM3 | KMM4 | |
---|---|---|---|---|
Plaques properties | Small, round, and clear Size: 0.8–1 mm | Small, round, and clear Size: 1–1.5 mm | Big, round with a clear center and surrounding turbid halo Size: 2.5–3.5 mm | Small, round, and clear Size: 1.2–1.5 mm |
Phage morphology Tail length (nm) Head width (nm) Head shape | 176.4 ± 8.4 75.8 ± 3 icosahedral | 90.9 ± 3.9 58.2 ± 2.7 icosahedral | 131.7 ± 9.3 50 ± 1.9 icosahedral | 90.5 ± 4.5 57.5 ± 2.8 icosahedral |
Latent period (min) | 27 | 20 | 45 | 30 |
Burst size (phages/infected bacterial cell) | 55 | 280 | 60 | 120 |
Taxonomy prediction | Caudoviricetes (Myovirus-like) | Caudoviricetes (Myovirus-like) | Caudoviricetes (Siphovirus-like) | Caudoviricetes (Myovirus-like) |
Bacteriophage | Phage Titre, 0 min (pfu/mL) | Phage Titre, 5 min (pfu/mL) | % of Adsorbed Phages | Adsorption Constant, k (mL/min) |
---|---|---|---|---|
KMM1 | 2.3 × 107 ± 1.2 × 107 | 3.4 × 104 ± 3.9 × 104 | 99.9 | 1.08 × 10−7 |
KMM2 | 3.1 × 107 ± 2.0 × 107 | 4.8 × 104 ± 1.2 × 104 | 99.9 | 4.62 × 10−8 |
KMM3 | 2.0 × 107 ± 1.3 × 107 | 5.7 × 103 ± 4.7 × 102 | 99.8 | 1.26 × 10−7 |
KMM4 | 4.5 × 107 ± 2.8 × 107 | 1.4 × 105 ± 1.3 × 105 | 99.8 | 8.70 × 10−8 |
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Share and Cite
Stante, M.; Weiland-Bräuer, N.; Repnik, U.; Werner, A.; Bramkamp, M.; Chibani, C.M.; Schmitz, R.A. Four Novel Caudoviricetes Bacteriophages Isolated from Baltic Sea Water Infect Colonizers of Aurelia aurita. Viruses 2023, 15, 1525. https://doi.org/10.3390/v15071525
Stante M, Weiland-Bräuer N, Repnik U, Werner A, Bramkamp M, Chibani CM, Schmitz RA. Four Novel Caudoviricetes Bacteriophages Isolated from Baltic Sea Water Infect Colonizers of Aurelia aurita. Viruses. 2023; 15(7):1525. https://doi.org/10.3390/v15071525
Chicago/Turabian StyleStante, Melissa, Nancy Weiland-Bräuer, Urska Repnik, Almut Werner, Marc Bramkamp, Cynthia M. Chibani, and Ruth A. Schmitz. 2023. "Four Novel Caudoviricetes Bacteriophages Isolated from Baltic Sea Water Infect Colonizers of Aurelia aurita" Viruses 15, no. 7: 1525. https://doi.org/10.3390/v15071525