Full Genome Characterization of the First Oropouche Virus Isolate Imported in Europe from Cuba
Abstract
:1. Introduction
2. Materials and Methods
2.1. Virus Isolation
2.2. RNA Extraction, Library Preparation and Sequencing
2.3. Bioinformatic Analysis
2.3.1. Reads Processing, Assembly and Consensus
2.3.2. Variant Calling and Annotation
2.3.3. GenBank Data Download
2.3.4. Phylogenetic Analysis and Genetic Variability
3. Results
3.1. Quality Control (QC) Analyses, Read Alignment and Consensus Generation
3.2. Variant Calling and Annotation
3.3. Phylogenetic Analysis and Genetic Variability
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Accession ID | Total Number of Sequenced Fragments | Fragments Mapped on OROV (n) | Coverage (%) | Mean Depth Coverage (X) | 10× (%) | 30× (%) | 1000× (%) | |
---|---|---|---|---|---|---|---|---|
S | PP952117 | 88,041,642 | 5632 | 99.27 | 606 | 99.16 | 98.95 | 19.41 |
M | PP952118 | 88,041,642 | 34,481 | 99.73 | 991 | 99.15 | 92.26 | 38.15 |
L | PP952119 | 88,041,642 | 15,899 | 99.31 | 246 | 97.03 | 93.03 | 4.27 |
Accession ID | Assembly Length | Coverage (%) | # SNVs | # Dels. | # Ins. | # Missense | # Synonymous | |
---|---|---|---|---|---|---|---|---|
S | PP952117 | 951 | 99.27 | 38 | 1 | 0 | 2 | 34 |
M | PP952118 | 4400 | 99.73 | 165 | 0 | 0 | 23 | 138 |
L | PP952119 | 7700 | 99.32 | 727 | 0 | 1 | 54 | 637 |
Gen I | Gen II | Gen III | Gen IV | Mean Intragroup Divergence | |
---|---|---|---|---|---|
Gen I | - | 0.024 | |||
Gen II | 5.0 ± 0.6 | - | 0.018 | ||
Gen III | 5.9 ± 0.7 | 3.9 ± 0.6 | - | 0.007 | |
Gen IV | 6.2 ± 0.8 | 5.6 ± 0.7 | 6.4 ± 0.9 | - | 0.004 |
OROV_SCDC_2024 | 5.1 ± 0.6 | 3.4 ± 0.5 | 4.8 ± 0.7 | 6.0 ± 0.8 | 0.010 |
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Deiana, M.; Malagò, S.; Mori, A.; Accordini, S.; Matucci, A.; Passarelli Mantovani, R.; Gianesini, N.; Huits, R.; Piubelli, C.; Gobbi, F.G.; et al. Full Genome Characterization of the First Oropouche Virus Isolate Imported in Europe from Cuba. Viruses 2024, 16, 1586. https://doi.org/10.3390/v16101586
Deiana M, Malagò S, Mori A, Accordini S, Matucci A, Passarelli Mantovani R, Gianesini N, Huits R, Piubelli C, Gobbi FG, et al. Full Genome Characterization of the First Oropouche Virus Isolate Imported in Europe from Cuba. Viruses. 2024; 16(10):1586. https://doi.org/10.3390/v16101586
Chicago/Turabian StyleDeiana, Michela, Simone Malagò, Antonio Mori, Silvia Accordini, Andrea Matucci, Rebeca Passarelli Mantovani, Natasha Gianesini, Ralph Huits, Chiara Piubelli, Federico Giovanni Gobbi, and et al. 2024. "Full Genome Characterization of the First Oropouche Virus Isolate Imported in Europe from Cuba" Viruses 16, no. 10: 1586. https://doi.org/10.3390/v16101586