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Article

Involvement of the Cell Division Protein DamX in the Infection Process of Bacteriophage T4

Institute of Biology, University of Hohenheim, 190h, Garbenstr. 30, 70599 Stuttgart, Germany
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Author to whom correspondence should be addressed.
Viruses 2024, 16(4), 487; https://doi.org/10.3390/v16040487
Submission received: 30 January 2024 / Revised: 18 March 2024 / Accepted: 19 March 2024 / Published: 22 March 2024
(This article belongs to the Special Issue Phage Assembly Pathways — to the Memory of Lindsay Black 2.0)

Abstract

The molecular mechanism of how the infecting DNA of bacteriophage T4 passes from the capsid through the bacterial cell wall and enters the cytoplasm is essentially unknown. After adsorption, the short tail fibers of the infecting phage extend from the baseplate and trigger the contraction of the tail sheath, leading to a puncturing of the outer membrane by the tail tip needle composed of the proteins gp5.4, gp5 and gp27. To explore the events that occur in the periplasm and at the inner membrane, we constructed T4 phages that have a modified gp27 in their tail tip with a His-tag. Shortly after infection with these phages, cells were chemically cross-linked and solubilized. The cross-linked products were affinity-purified on a nickel column and the co-purified proteins were identified by mass spectrometry, and we found that predominantly the inner membrane proteins DamX, SdhA and PpiD were cross-linked. The same partner proteins were identified when purified gp27 was added to Escherichia coli spheroplasts, suggesting a direct protein–protein interaction.
Keywords: bacteriophage T4; DNA translocation; baseplate; tail tip; inner membrane proteins bacteriophage T4; DNA translocation; baseplate; tail tip; inner membrane proteins

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MDPI and ACS Style

Wenzel, S.; Hess, R.; Kiefer, D.; Kuhn, A. Involvement of the Cell Division Protein DamX in the Infection Process of Bacteriophage T4. Viruses 2024, 16, 487. https://doi.org/10.3390/v16040487

AMA Style

Wenzel S, Hess R, Kiefer D, Kuhn A. Involvement of the Cell Division Protein DamX in the Infection Process of Bacteriophage T4. Viruses. 2024; 16(4):487. https://doi.org/10.3390/v16040487

Chicago/Turabian Style

Wenzel, Sabrina, Renate Hess, Dorothee Kiefer, and Andreas Kuhn. 2024. "Involvement of the Cell Division Protein DamX in the Infection Process of Bacteriophage T4" Viruses 16, no. 4: 487. https://doi.org/10.3390/v16040487

APA Style

Wenzel, S., Hess, R., Kiefer, D., & Kuhn, A. (2024). Involvement of the Cell Division Protein DamX in the Infection Process of Bacteriophage T4. Viruses, 16(4), 487. https://doi.org/10.3390/v16040487

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