Discovery and Genomic Analysis of Three Novel Viruses in the Order Mononegavirales in Leafhoppers
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Preparation and RNA Extraction
2.2. Bioinformatic Analysis
2.3. Obtaining the Full-Length Virus Genomes through RACE and Validation of Virus Genome
2.4. Sequence and Phylogenetic Analyses
3. Results
3.1. Transcriptome Assembly and Virus Discovery after Sample Collection
3.2. Characterization of Two Novel Viruses of the Family Rhabdoviridae
3.3. Characterization of a Novel Member of the Family Lispiviridae
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Xiao, J.; Nie, B.; Chen, M.-E.; Ge, D.; Liu, R. Discovery and Genomic Analysis of Three Novel Viruses in the Order Mononegavirales in Leafhoppers. Viruses 2024, 16, 1321. https://doi.org/10.3390/v16081321
Xiao J, Nie B, Chen M-E, Ge D, Liu R. Discovery and Genomic Analysis of Three Novel Viruses in the Order Mononegavirales in Leafhoppers. Viruses. 2024; 16(8):1321. https://doi.org/10.3390/v16081321
Chicago/Turabian StyleXiao, Jiajing, Binghua Nie, Meng-En Chen, Danfeng Ge, and Renyi Liu. 2024. "Discovery and Genomic Analysis of Three Novel Viruses in the Order Mononegavirales in Leafhoppers" Viruses 16, no. 8: 1321. https://doi.org/10.3390/v16081321