Laboratory Replication of Ostreid Herpes Virus (OsHV-1) Using Pacific Oyster Tissue Explants
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of OsHV-1 Stock
2.2. Animal Husbandry
2.3. Whole Animal Experimental Infections and Oyster Site Comparisons
2.4. Attenuation of Viral Infectivity in Seawater
2.5. Explant Infection
2.6. Tissue Fixation and OsHV-1 Quantification
2.7. Media OsHV-1 Quantification
2.8. Degradation of OsHV-1 DNA
2.9. Transmission Electron Microscopy
2.10. Statistical Analysis
3. Results
3.1. Attenuation of Viral Infectivity in Seawater
3.2. Degredation of Viral DNA in Water
3.3. Comparison of Mortality from Different Oyster Sources
3.4. OsHV-1 Replication in Tissue Explant Cultures
3.5. Quantification in Tissue VS Media
3.6. Electron Microscopy
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Potts, R.W.A.; Regan, T.; Ross, S.; Bateman, K.; Hooper, C.; Paley, R.; Houston, R.D.; Bean, T.P. Laboratory Replication of Ostreid Herpes Virus (OsHV-1) Using Pacific Oyster Tissue Explants. Viruses 2024, 16, 1343. https://doi.org/10.3390/v16081343
Potts RWA, Regan T, Ross S, Bateman K, Hooper C, Paley R, Houston RD, Bean TP. Laboratory Replication of Ostreid Herpes Virus (OsHV-1) Using Pacific Oyster Tissue Explants. Viruses. 2024; 16(8):1343. https://doi.org/10.3390/v16081343
Chicago/Turabian StylePotts, Robert W. A., Tim Regan, Stuart Ross, Kelly Bateman, Chantelle Hooper, Richard Paley, Ross D. Houston, and Tim P. Bean. 2024. "Laboratory Replication of Ostreid Herpes Virus (OsHV-1) Using Pacific Oyster Tissue Explants" Viruses 16, no. 8: 1343. https://doi.org/10.3390/v16081343