Cytokine Signatures in Inflamed Mucosa of IBD Patients: State-of-the-Art

Round 1

Reviewer 1 Report

Comments and Suggestions for Authors

A- The review article revolves around illustrating important techniques involved in cytokine profile analysis on different markers during inflammatory responses in IBD patients. A- The topic taps into the techniques and their relevance in IBD. Though the techniques have been used for a long time for studying inflammation, this review talks specifically on their importance in IBD and related diseases. Therefore it does address a specific area where more can be looked into in the future while researching on IBD. A- The other published material talks specifically on related inflammatory insights into the IBD as a disease but there seems to be an area where the techniques involved during IBD can be looked into, which this review specifically talks about. The manuscript is well-written, describes in detail, with the figure and table. Therefore it can be accepted in its current form.

Author Response

Authors’ response: We thank Reviewer 1 for the positive comments and appreciation. The encouraging words validate the effort we have put into this research and provide valuable motivation for our ongoing and future work. We are grateful for the recognition of the significance and quality of our study, and we look forward to addressing any further suggestions or insights the referee may have.

Reviewer 2 Report

Comments and Suggestions for Authors

The manuscript by Milena Peruhova et al provided a very comprehensive review of state-of-the-art techniques for cytokine profiling in IBD patients, as well as the mainstay treatments for IBD and their impact on cytokine modulation. First, the authors concisely summarized the role of mucosal immunology in IBD pathogenesis. They further thoroughly and comprehensively discussed state-of-the-art techniques for cytokine profiling in IBD patients, including microarray, RNA-seq and scRNA-seq, mass cytometry (CyTOF), and multiplex cytokine assays (Luminex). Clinical implications and therapeutic opportunities in IBD have also been discussed and summarized. Lastly, The manuscript also summarized various current IBD therapies targeting cytokines in IBD patients and their impact on cytokines.

Overall, it is a well-written, well-balanced review, with most recent findings incorporated and critically discussed, and it provides highly significant clinical implications for IBD treatments.

I have no significant criticisms.

 

A minor point:

In several places (such as in lines -84, -371, -405, -478), for “Inflammatory Bowel 84 Disease (IBD)”, please just use IBD for consistency.

Author Response

Authors’ response: We thank Reviewer 2 for the careful work for improving the quality of our manuscript. Their insightful comments have been instrumental in enhancing the clarity and rigor of our work. We appreciate the constructive feedback and have made the appropriate corrections as suggested. We are grateful for the valuable contribution to our paper.