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Thalassemia Reports
Volume 4
Issue 2
10.4081/thal.2014.1837
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Article

Differential Regulation of Plasma Proteins between Members of a Family with Homozygous HbE and HbEβ-thalassemia

by
Suchismita Halder
1,
Tridip Chatterjee
2,
Amit Chakravarty
2,
Sudipa Chakravarty
2 and
Abhijit Chakrabarti
3,*
1
Biophysics & Structural Genomics Division, Saha Institute of Nuclear Physics, Kolkata 700064, India
2
Institute of Genetic Medicine and Genomic Science, Kolkata 700064, India
3
Crstallography and Molecular Biology Division, Saha Institute of Nuclear Physics, Kolkata 700064, India
*
Author to whom correspondence should be addressed.
Thalass. Rep. 2014, 4(2), 1837; https://doi.org/10.4081/thal.2014.1837
Submission received: 22 July 2013 / Revised: 18 November 2013 / Accepted: 11 February 2014 / Published: 29 September 2014

Abstract

In this report we’ve compared the plasma protein profiles of 4 individuals in a family. Father and the younger son both are hemoglobin (Hb) Eβ-thalassemic {Cod 26 (G-A)/IVS 1- 5 (G-C)}, but the father never requires transfusion, whereas the younger son requires monthly blood transfusion. Mother and the elder son are HbEE {Cod 26 (G-A)/Cod 26 (GA)} without any history of transfusion. Proteomic study was done on the plasma fraction of the blood following ammonium sulphate precipitation. Proteins were separated by 2D-gel electrophoresis, expression of proteins compared by densitometry and proteins identified by tandem MALDI mass spectrometry. Proteins responsible in hemolysis, hypercoagulation and hemoglobin scavenging have shown differential regulation, establishing the relation between the differences in the levels of plasma proteins with the progression of the disease phenotype, manifested in the extent of transfusion dependence of the patient.
Keywords: HbE-β thalassemia; blood transfusion; differential proteomics HbE-β thalassemia; blood transfusion; differential proteomics

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MDPI and ACS Style

Halder, S.; Chatterjee, T.; Chakravarty, A.; Chakravarty, S.; Chakrabarti, A. Differential Regulation of Plasma Proteins between Members of a Family with Homozygous HbE and HbEβ-thalassemia. Thalass. Rep. 2014, 4, 1837. https://doi.org/10.4081/thal.2014.1837

AMA Style

Halder S, Chatterjee T, Chakravarty A, Chakravarty S, Chakrabarti A. Differential Regulation of Plasma Proteins between Members of a Family with Homozygous HbE and HbEβ-thalassemia. Thalassemia Reports. 2014; 4(2):1837. https://doi.org/10.4081/thal.2014.1837

Chicago/Turabian Style

Halder, Suchismita, Tridip Chatterjee, Amit Chakravarty, Sudipa Chakravarty, and Abhijit Chakrabarti. 2014. "Differential Regulation of Plasma Proteins between Members of a Family with Homozygous HbE and HbEβ-thalassemia" Thalassemia Reports 4, no. 2: 1837. https://doi.org/10.4081/thal.2014.1837

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