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Clinics and Practice
Volume 4
Issue 1
10.4081/cp.2014.608
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Clinics and Practice is published by MDPI from Volume 11 Issue 1 (2021). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with PAGEPress.
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Case Report

Assessment of Folic Acid and DNA Damage in Cleft Lip and Cleft Palate

by
Sivakumar Brooklyin
1,
Rashmoni Jana
1,*,
Singaravelu Aravinthan
1,
Bethou Adhisivam
2 and
Parkash Chand
1
1
Department of Anatomy, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), India
2
Department of Pediatrics, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), India
*
Author to whom correspondence should be addressed.
Clin. Pract. 2014, 4(1), 608; https://doi.org/10.4081/cp.2014.608
Submission received: 31 October 2013 / Revised: 21 January 2014 / Accepted: 25 February 2014 / Published: 31 March 2014

Abstract

Studies have identified the risk factors like folic acid deficiency during gestational period, family history for orofacial clefts, drugs like antiepileptic, vitamin A. But, the data regarding the folic acid status in children with cleft lip/palate is hardly evaluated in depth. Here, an assessment of folic acid and DNA damage were carried out in children with orofacial anomalies. Folic acid level and DNA damage were evaluated by folic acid assay (direct chemiluminescent technology) and single cell gel electrophoresis or comet assay method respectively. The mean value of plasma folic acid by direct chemiluminescent technology was 6.5±3.6 nmol/L and the normal value in children ranges from 11.3 to 47.6 nmol/L. The amount of damaged DNA, measured as the tail length of the comet in cases, was 19.4±8.9 ?m and the mean percentage of DNA in tail was 16.5±3.7. Folic acid deficiency could be the reason for DNA damage.
Keywords: cleft lip; cleft palate; comet assay; orofacial clefts; MTHFR gene cleft lip; cleft palate; comet assay; orofacial clefts; MTHFR gene

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MDPI and ACS Style

Brooklyin, S.; Jana, R.; Aravinthan, S.; Adhisivam, B.; Chand, P. Assessment of Folic Acid and DNA Damage in Cleft Lip and Cleft Palate. Clin. Pract. 2014, 4, 608. https://doi.org/10.4081/cp.2014.608

AMA Style

Brooklyin S, Jana R, Aravinthan S, Adhisivam B, Chand P. Assessment of Folic Acid and DNA Damage in Cleft Lip and Cleft Palate. Clinics and Practice. 2014; 4(1):608. https://doi.org/10.4081/cp.2014.608

Chicago/Turabian Style

Brooklyin, Sivakumar, Rashmoni Jana, Singaravelu Aravinthan, Bethou Adhisivam, and Parkash Chand. 2014. "Assessment of Folic Acid and DNA Damage in Cleft Lip and Cleft Palate" Clinics and Practice 4, no. 1: 608. https://doi.org/10.4081/cp.2014.608

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