Occurrence and Identification of Aspergillus Section Flavi in the Context of the Emergence of Aflatoxins in French Maize
Abstract
:1. Introduction
2. Results
2.1. Climatic Data
2.2. Occurrence of Aflatoxins in Maize Samples
2.2.1. Number and Location of Contaminated Samples
2.2.2. Types and Levels of AF Contamination
2.3. Fungal Flora of Maize Samples
2.3.1. AF+ Samples
2.3.2. AF− Samples
2.3.3. Quantitative Comparison
2.4. Characterization of Aspergillus Section Flavi Strains
3. Discussion
3.1. Impact of Climate on Aflatoxin Emergence in French Maize in 2015
3.2. Nature of Aspergillus Section Flavi Responsible for Aflatoxin Contamination of French Maize
4. Conclusions
5. Materials and Methods
5.1. Meteorological Data
5.2. Sampling
5.2.1. Sampling Strategy
5.2.2. Sample Collection
Field Samples
Silo Samples
5.2.3. Sample Preparation for Analysis
5.3. Aflatoxin and Cyclopiazonic Acid Quantification
5.4. Fungal Count and Identification
5.5. Characterization of Aspergillus Section Flavi
5.5.1. Morphological Identification
5.5.2. Molecular Identification
5.5.3. Mycotoxigenic Potential of Isolates
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Origin | Contaminated Samples (%) | Aflatoxin Content (µg/kg) | ||||
---|---|---|---|---|---|---|
AFB1 | AFB2 | AFG1 | AFG2 | Total AFs | ||
1.1 | ND | 0.4 | ND | 1.5 | ||
0.1 | ND | 0.2 | ND | 0.3 | ||
4.0 | 0.4 | 9.7 | 1.2 | 15.3 | ||
FIELD | 7 samples/118 | 0.3 | ND | 2.0 | 0.3 | 2.6 |
6% | 0.7 | ND | 0.5 | ND | 1.2 | |
20.4 | 2.1 | 24.8 | 3.5 | 50.8 | ||
66.0 | 3.1 | 0.9 | ND | 70 | ||
0.1 | ND | 0.7 | ND | 0.8 | ||
3.2 | 0.2 | 4.5 | ND | 7.9 | ||
0.6 | ND | ND | ND | 0.6 | ||
3.3 | 0.2 | ND | ND | 3.5 | ||
2.4 | 0.2 | ND | ND | 2.6 | ||
SILOS | 12 samples/81 | 1.4 | ND | 0.1 | ND | 1.5 |
15% | 3.8 | 0.2 | 0.8 | ND | 4.8 | |
0.6 | ND | ND | ND | 0.6 | ||
1.9 | 0.2 | 3.9 | 0.6 | 6.6 | ||
7.2 | 0.3 | 5.6 | 0.2 | 13.3 | ||
0.4 | ND | 0.1 | ND | 0.5 | ||
0.7 | ND | ND | ND | 0.7 |
Mycoflora | Mean Fungal Load (CFU/g) (Mini–Maxi) % Contaminated Samples | ||
---|---|---|---|
AF− Field Samples (n = 24) | AF+ Field Samples (n = 7) | AF+ Silo Samples (n = 12) | |
Total flora | 2.3 × 105 | 7.9 × 105 | 2 × 105 |
(1.2 × 104–1.2 × 106) | (6.9 × 104–2.5 × 106) | (1.4 × 104–7.7 × 105) | |
Fusarium sp. | 1.6 × 105 | 3.5 × 105 | 1.7 × 105 |
(103–5 × 105) | (104–2 × 106) | (104–7 × 105) | |
100% | 100% | 100% | |
Penicillium sp. | 3.5 × 104 | 3.2 × 105 | 6.1 × 103 |
(2 × 102–4 × 105) | (5 × 103–2 × 106) | (5 × 102–3 × 104) | |
100% | 100% | 100% | |
Acremonium sp. | 3.3 × 104 | 5.7 × 104 | 1.1 × 104 |
(0–3 × 105) | (0–2 × 105) | (0–3 × 104) | |
67% | 86% | 58% | |
Cladosporium sp. | 2.2 × 103 | 4.2 × 103 | 1.3 × 102 |
(0–104) | (0–2 × 104) | (0–103) | |
79% | 86% | 25% | |
Aspergillus sp. | 2.2 × 103 | 5.3 × 104 | 9.2 × 103 |
(0–2 × 104) | (2 × 103–1.7 × 105) | (102–4 × 104) | |
54% | 100% | 100% | |
Aspergillus section Nigri | 103 | 2.1 × 104 | 5.8 × 102 |
(0–2 × 104) | (102–7 × 104) | (0–4 × 103) | |
46% | 100% | 83% | |
Aspergillus section Flavi | 9.9 × 102 | 3.2 × 104 | 6.2 × 103 |
(0–2 × 104) | (6 × 102–105) | (102–3 × 104) | |
38% | 100% | 100% |
Gene | Gene Name | Length (bp) | Primers | Sequence (Nucleotides: 5’→3’) | |
---|---|---|---|---|---|
Forward | Reverse | ||||
ITS (4–5) | Internal transcribed spacer | 300–330 | ITS5 | 5′-GGAAGTAAAAGTCGTAACAAGG | |
ITS 4 | 5′-TCCTCCGCTTATTGATATGC | ||||
benA-2 | ß-tubulin | 1125 | benA 2a | 5′-GGTAACCAAATCGGTGCTGC | |
benA 2b | 5′-ACCCTCAGTGTAGTGACCCTTGGC | ||||
cmdA | Calmodulin | 543 | Cmd5 | 5′-CCGAGTACAAGGAGGCCTTC-3’ | |
Cmd6 | 5′-CCGATAGAGGTCATAACGTGG-3’ |
Species and Strain Number | Accession Number | ||
---|---|---|---|
A. parasiticus | ITS | cmdA | benA |
G622Rc | MK165710 | MK165730 | MK172077 |
G622Rd | MK165711 | MK165731 | MK172078 |
G628Ra | MK165712 | MK165732 | MK172079 |
G632b | MK165713 | MK165733 | MK172080 |
G632c | MK165714 | MK165734 | MK172081 |
G638b | MK165709 | MK165735 | MK172082 |
G639 | MK165726 | MK165736 | MK172083 |
G640a | MK165715 | MK165737 | MK172084 |
G641b | MK165716 | MK165738 | MK172085 |
G643Rb | MK165717 | MK165739 | MK172086 |
G644b | MK165718 | MK165740 | MK172087 |
G647Rc | MK165719 | MK165741 | MK172088 |
G648b | MK165720 | MK172072 | MK172089 |
G649a | MK165708 | MK165742 | MK172090 |
G649b | MK165721 | MK165743 | MK172091 |
G650d | MK165722 | MK165744 | MK172092 |
G651a | MK165723 | MK165745 | MK172093 |
G651e | MK165724 | MK172073 | MK172094 |
G652b | MK165725 | MK172096 | MK172095 |
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Bailly, S.; Mahgubi, A.E.; Carvajal-Campos, A.; Lorber, S.; Puel, O.; Oswald, I.P.; Bailly, J.-D.; Orlando, B. Occurrence and Identification of Aspergillus Section Flavi in the Context of the Emergence of Aflatoxins in French Maize. Toxins 2018, 10, 525. https://doi.org/10.3390/toxins10120525
Bailly S, Mahgubi AE, Carvajal-Campos A, Lorber S, Puel O, Oswald IP, Bailly J-D, Orlando B. Occurrence and Identification of Aspergillus Section Flavi in the Context of the Emergence of Aflatoxins in French Maize. Toxins. 2018; 10(12):525. https://doi.org/10.3390/toxins10120525
Chicago/Turabian StyleBailly, Sylviane, Anwar El Mahgubi, Amaranta Carvajal-Campos, Sophie Lorber, Olivier Puel, Isabelle P. Oswald, Jean-Denis Bailly, and Béatrice Orlando. 2018. "Occurrence and Identification of Aspergillus Section Flavi in the Context of the Emergence of Aflatoxins in French Maize" Toxins 10, no. 12: 525. https://doi.org/10.3390/toxins10120525