Mycotoxins at the Start of the Food Chain in Costa Rica: Analysis of Six Fusarium Toxins and Ochratoxin A between 2013 and 2017 in Animal Feed and Aflatoxin M1 in Dairy Products

Molina, Andrea; Chavarría, Guadalupe; Alfaro-Cascante, Margarita; Leiva, Astrid; Granados-Chinchilla, Fabio

doi:10.3390/toxins11060312

Open AccessEditor’s ChoiceArticle

Mycotoxins at the Start of the Food Chain in Costa Rica: Analysis of Six Fusarium Toxins and Ochratoxin A between 2013 and 2017 in Animal Feed and Aflatoxin M₁ in Dairy Products

by

Andrea Molina

^1,2,

Guadalupe Chavarría

¹,

Margarita Alfaro-Cascante

¹,

Astrid Leiva

¹

and

Fabio Granados-Chinchilla

^1,*

¹

Centro de Investigación en Nutrición Animal (CINA), Universidad de Costa Rica, Ciudad Universitaria Rodrigo, San José 11501-2060, Costa Rica

²

Escuela de Zootecnia, Universidad de Costa Rica, Ciudad Universitaria Rodrigo, San José 11501-2060, Costa Rica

^*

Author to whom correspondence should be addressed.

Toxins 2019, 11(6), 312; https://doi.org/10.3390/toxins11060312

Submission received: 2 March 2019 / Revised: 16 April 2019 / Accepted: 17 April 2019 / Published: 31 May 2019

(This article belongs to the Special Issue Mycotoxin Exposure and Related Diseases)

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Abstract

:

Mycotoxins are secondary metabolites, produced by fungi of genera Aspergillus, Penicillium and Fusarium (among others), which produce adverse health effects on humans and animals (carcinogenic, teratogenic and immunosuppressive). In addition, mycotoxins negatively affect the productive parameters of livestock (e.g., weight, food consumption, and food conversion). Epidemiological studies are considered necessary to assist stakeholders with the process of decision-making regarding the control of mycotoxins in processing environments. This study addressed the prevalence in feed ingredients and compound feed of eight different types of toxins, including metabolites produced by Fusarium spp. (Deoxynivalenol/3-acetyldeoxynivalenol, T-2/HT-2 toxins, zearalenone and fumonisins) and two additional toxins (i.e., ochratoxin A (OTA) and aflatoxin M₁ (AFM₁)) from different fungal species, for over a period of five years. On the subject of Fusarium toxins, higher prevalences were observed for fumonisins (n = 80/113, 70.8%) and DON (n = 212/363, 58.4%), whereas, for OTA, a prevalence of 40.56% was found (n = 146/360). In the case of raw material, mycotoxin contamination exceeding recommended values were observed in cornmeal for HT-2 toxin (n = 3/24, 12.5%), T-2 toxin (n = 3/61, 4.9%), and ZEA (n = 2/45, 4.4%). In contrast, many compound feed samples exceeded recommended values; in dairy cattle feed toxins such as DON (n = 5/147, 3.4%), ZEA (n = 6/150, 4.0%), T-2 toxin (n = 10/171, 5.9%), and HT-2 toxin (n = 13/132, 9.8%) were observed in high amounts. OTA was the most common compound accompanying Fusarium toxins (i.e., 16.67% of co-occurrence with ZEA). This study also provided epidemiological data for AFM₁ in liquid milk. The outcomes unveiled a high prevalence of contamination (i.e., 29.6–71.1%) and several samples exceeding the regulatory threshold. Statistical analysis exposed no significant climate effect connected to the prevalence of diverse types of mycotoxins.

Keywords:

Fusarium mycotoxins co-contamination; ochratoxin A; feed prevalence and safety; HPLC analysis

Key Contribution: This study generated essential epidemiological and toxicological evidence about the individual and combined occurrence of Fusarium mycotoxins and ochratoxin A in feedstuffs in Costa Rica. These findings portray imperative implications for all stakeholders linked to the feed industry as well as supplies for improving the management of mycotoxins in animal production.

1. Introduction

Mycotoxins are toxic fungal metabolites that can be found in feed ingredients and compound feeds [1,2]. Due to their compositions, they are detrimental to animal and human health [3,4,5,6,7,8]. Currently, more than 400 different types of mycotoxins have been identified [9]. However, Fusarium toxins are among the most commonly monitored as they are acknowledged to present serious health concerns [7,10]. Under certain conditions, some fungi can produce several toxins simultaneously [11,12,13].

In feed production, ca. 60% of the formulation consists of cornmeal, soybean meal, and their derivates [14,15]. In Costa Rica, cereal production represents 38% of the agricultural sector imports [16], where its main suppliers are the United States and Brazil with 84% and 15% contribution, respectively [17]. In this regard, corn imports have increased from 738,539.97 to 781,903.54 metric tons from 2015 to 2017 [18]. On the other hand, soybean imports have risen to 309,897.97 metric tons per year, even though 83% of the soybean meal used as a feedstuff comes from national production [18]. Furthermore, only 38% of the products destined for animal consumption are from national origin, representing a total feed production of 1,238,243 metric tons in 2017. Approximately 45%, 27%, 20%, and 4% of this production is intended to be destined to poultry, higher ruminants, swine, and pets (i.e., cats and dogs), respectively [18]. That is, import and export of animal feed and feed ingredients play an essential part in the co-occurrence of various types of mycotoxins in the finished feed [19,20]. Hence, co-occurrence could be a far more certain and prevalent issue in real mycotoxin feed analysis [11,12,20,21,22,23].

Mycotoxin metabolites retain toxicity and thus must be surveilled [24,25]. Mycotoxins and their metabolites have several implications for animal and human health. Some are identified/classified as teratogenic, genotoxic, carcinogenic, and immunotoxic. The ingestion of contaminated feed affects animal health and may reduce productivity in animals, generating economic losses [26]. Some mycotoxins ingested and metabolized by productive animals could be accumulated in different organs and tissues reaching the food chain through meat, milk, or eggs [24,27,28]. In Costa Rica, during 2018, consumption of these commodities was estimated in 58.7 kg (i.e., 14.3, 15.4, and 29 kg year⁻¹ for cattle, pork, and chicken, respectively), 215 L, and 218 units per capita, individually [18].

In this regard, epidemiological information tends to be more comprehensive when exploring data from several toxins simultaneously [29]. Accurate mycotoxin data about their presence in feeds are paramount for stakeholders’ decision-making process towards the risk management in their manipulation [30]. Numerous reports have explicitly documented the incidence of mycotoxins in feeds, especially in Europe [11,31,32], USA [33], Asia [31], and China [34]. Nowadays, there are insufficient reports oriented to describe the incidence of mycotoxins in feed in Costa Rica. The emphasis has been made towards the investigation of aflatoxins [35,36].

Herein, the prevalent data from feed and feed ingredient samples of eight different toxins, mainly produced by Fusarium spp. (deoxynivalenol/3-acetyldexoynivalenol (DON/3-ADON), T-2/HT-2 toxins, zearalenone (ZEA) and fumonisins (FB₁ and FB₂)), but also ochratoxin A (OTA), during five years are provided. Finally, in the same period, we analyzed the behavior of AFM₁ in liquid milk.

2. Results

2.1. Fusarium Toxins Present in Animal Feed

The highest prevalence of Fusarium toxins during the analyzed period (2012–2017) was observed for fumonisin and DON in 70.8% (n = 80/113) and 58.4% (n = 212/363) of the cases, respectively. For FB₁ + FB₂ the prevalence ranged from 27.8% (n = 5/18) in 2013 to 85.2% (n = 23/27) in 2014, with a maximum concentration of 53,580 µg kg⁻¹ observed in 2015. The prevalence for DON ranged from 42.0% (n = 40/94) in 2016 to 79.3% (n = 69/87) in 2014, with a maximum concentration of 151,060 µg kg⁻¹ presented in 2013 (Table 1). Lower prevalences of 21.2% (n = 45/212) and 36.1% (n = 97/269) with a maximum mycotoxin level of 16,100 µg kg⁻¹ (in 2015) and 12,500 µg kg⁻¹ (in 2014) were observed for 3-acetyldeoxynivalenol and HT-2, respectively (Table 1). Concentration-wise and among periods, ZEA and T-2 toxin increased meaningfully in 2017 and 2013, respectively. For HT-2, OTA, DON, 3-ADON, FB₁, FB₂, and FB₁ + FB₂, no differences were observed.

2.2. Mycotoxin Prevalence in Feed Ingredients

In the matter of feed ingredients, cornmeal exceeded guideline values for HT-2 toxin (n = 3/24, 12.5%), T-2 toxin (n = 3/61, 4.9%), and ZEA (n = 2/45, 4.4%) (Table 2). In a soybean meal, merely HT-2 toxin (n = 1/6, 16.7%) was detected in this situation, and just one sample of wheat had an excessive amount of DON (n = 1/8, 12.5%) (Table 2). With reference to other raw materials, of less inclusion, such as rice byproducts, palm oil byproducts, of the citrus industry, as well as forages, silages, and hays (treated as a whole group), there are no regulatory guidelines to establish an acceptance parameter. However, it is interesting to notice that, in the groups described above, they share as a common feature a high prevalence of DON (i.e., 66.7%) (Table 2).

2.3. Mycotoxin Prevalence in Compound Feed

Among compound feeds, beef cattle feed presented only a few samples above the guideline level (specifically, T-2 and HT-2 toxin, n = 2/63, 3.2%). Dairy cattle feed presented the highest number of samples that surpassed the recommended levels of mycotoxins (n = 34/105, 32.4%), specifically DON (n = 5/147, 3.4%), ZEA (n = 6/150, 4.0%), T-2 toxin (n = 10/171, 5.8%) and HT-2 (n = 13/132, 9.8%) (Table 3). Poultry feed presented only 10 samples exceeding the guidelines, for DON (n = 2/14, 14.3%), FB₁ (n = 1/7, 14.3%), HT-2 toxin (n = 1/15, 6.7%), and OTA (n = 1/9, 11.1%). Cat and dog food also showed values above legal thresholds for fumonisins (n = 6/13, 46.1%), with a maximum of 18,910 µg kg⁻¹ (Table 3). The second highest prevalence was observed connected with swine feed (n = 14/71, 19.7%) with the mycotoxins ZEA (n = 2/18, 11.2%), FB₁ (n = 2/9, 22.2%), and DON (n = 6/17, 35.3%) infringing the respective recommended guidelines (Table 2). Fish feed also exceeded thresholds for DON (n = 2/16, 12.5%). Finally, in horse feed, Fumonisin B₂ was found (n = 1/26, 3.8%) (Table 3).

2.4. Geographical Distribution and Climate Influence for Fusarium Toxins Present in Animal Feed

Geographical and national toxin hotspot distribution was similar for those toxins produced by Fusarium species (Figure 1A–G). A completely different profile was observed when studying OTA and AFM₁. Interestingly, only 3-ADON and HT-2 toxins prevailed during the rainy season. For other toxins, there were no differences in the levels of contamination between the dry season and the rainy season (Table 4). As expected, the co-occurrence of two different toxins was the most common situation (i.e., n = 141/279, 50.5%) (Table 5). Therefore, as the number of simultaneous toxins increased, co-occurrence was less likely to be found (Table 5). In the case of the parent compound–metabolite comparison, the most common combination was the pair T-2/HT-2 toxin with (n = 66/155) 42.6% of prevalence, followed by FB₁/FB₂ (n = 23/137, 16.8%) and DON/3-ADON (n = 18/177, 10.2%) (Table 5).

2.5. OTA Prevalence in Animal Feeds

Referring to OTA, the total prevalence from 2012 to 2017 was 40.6% (n = 146/360), ranging from 16.3% (n = 8/49) in 2013 to 76.6% (n = 49/64) in 2015. The maximum OTA reported level was 1810 µg kg⁻¹, in 2016 (Table 1). Only one sample exceeded the maximal advisory level for ochratoxin; this sample corresponded to poultry feed where the recommended concentration is 100 µg kg⁻¹. The overall OTA prevalence in non-traditional ingredients, poultry, and fish feed was of 56.3%, 44.4%, and 66.7%, respectively (Table 2 and Table 3). Furthermore, in May and September, the highest global concentrations of OTA were presented, corresponding to the rainy season releasing an evident difference compared with the findings of the dry season (Table 4). As the presence of OTA involves other toxin-producing fungi (other than Fusarium), co-occurrence with other metabolites is a possibility. The most prevalent Fusarium toxins present in feed (different from OTA), in decreasing order of incidence, were ZEA, DON + FB₁, FB₁, and T-2 toxin with (n = 17/102) 16.7%, (n = 14/102) 13.7%, and (n = 12/102) 11.8% of incidence, respectively (Table 5). As expected, OTA incidence had a completely different geographical/spatial (Figure 1H) and thermo/temporal (Figure 2H) distribution, when compared with the other toxins.

2.6. Aflatoxin M₁ in Liquid Milk

Water buffalo milk and butter samples were also analyzed for the presence of Aflatoxin M₁. Water buffalo (Bubalus bubalis) milk samples (n = 2) were reported below the limit of quantification (i.e., 0.014 µg kg⁻¹) and butter (n = 3) ranged from 0.021 to 0.024 µg kg⁻¹. Even though 2016 was the year with the lowest number of analyzed samples, it was also the year when fewer samples surpassed the 0.05 µg kg⁻¹ threshold (Table 6). An increase in AFM₁ prevalence with 71.1% and 63.2%, respectively (Table 6), was observed during 2014 and 2017. Excluding three samples from 2015, there were no other samples surpassing the US FDA threshold of 0.5 µg kg⁻¹, thus representing a very small overall percentage for the four years of the study (i.e., n = 3/175, 1.7%). It was studied/monitored that, consistently, higher concentrations of AFM₁ were obtained during March, August, and September (Table 6 and Figure 2I).

3. Discussion

3.1. Mycotoxin Prevalence between 2013 and 2017 in Animal Feed

Most of the studied toxins (except for 3-ADON, FB₁, and HT-2) had prevalences higher than 40% during the five years. The average concentrations found in the different toxins in animal feed did not vary between one year and another, except for ZEA and T-2. The drastic increase of ZEA concentrations during 2017 was observed in corn meal and sorghum silo. There is a prior documented avidity of Fusarium spp. to produce ZEA when using moderately alkaline cereals (e.g., maize) as substrates [43]. A general drop in annual temperature may have provoked this upsurge in ZEA contamination. For example, Fusarium graminearum has demonstrated that conditions of pH 9 and incubation temperature of 15.05 °C are required to favor ZEA production [44]. Interestingly, the most toxicologically relevant levels for ZEA were encountered at relatively low temperatures (i.e., near 15 °C). Despite a relatively high prevalence for mycotoxins (i.e., between 46% and 99%, except for FB₁ + FB₂ and DON), the positive samples possessed comparatively low concentrations (Table 1) based on guidance values for mycotoxins in animal feeds within the European Union (see Appendix A Table A1 and Table A2) [37,38]. This relatively low toxicological burden could be associated with the control of mycotoxin in animal feed and raw materials that were established in the country since 2007. This control policy covers the majority of the toxins analyzed in this study added to the control of imported raw materials, before its distribution. In coherence to what has been stated, since 2013, proficient manufacturing practices have been evaluated and audited by regulation in animal feed plants. These proficient practices involve the management of raw materials and storage measures, among others, contributing to the reduction of mycotoxin contamination [45].

However, some of the samples were observed with concentrations above the established guidelines with potentially adverse effects on animal health and productivity. It is worth of mentioning the fact that human health could be affected through the consumption of foods of animal origin contaminated with mycotoxins or their metabolites [24,27,28].

3.2. Mycotoxin Prevalence in Compound Feed and Feed Ingredients

3.2.1. Prevalence in Feed Ingredients

Vegetable ingredients may represent from 80% to 100% of the feed (e.g., in ruminants, animal origin ingredients are prohibited) [14,46,47]. For these vegetable-based formulations, corn and soybean meal may represent up to 60% of the input [14,15]. Costa Rican soybean meal and corn, as well as other relevant ingredients, are imported [18]. Quality grain assessment is a degree-based classification. Usually, grade 2 or 3 corn is purchased for feed production [18]. At least 97.9% of the samples contain around 3% of cracked material, and 36.2% of the samples exhibited higher moisture content (i.e., 17%); both factors promote the proliferation of fungi [48]. Toxin-wise, AFB_1, and DON were assayed and are regulated according to FDA criteria. Only 1.9% samples exceeded levels for AFB₁ but none for DON [49]. The data reveal coherence with the obtained results (Table 2). Notwithstanding, a high prevalence for DON was detected and reported by other researchers both for corn and wheat [49]. Conversely, a relatively lower incidence was found in OTA, different from what was conveyed elsewhere [50].

3.2.2. Prevalence in Cattle Feeds

In both dairy and meat cattle, forage, hay, and silage input must not be underplayed, especially in countries where extensive feeding systems based on grazing cattle predominate. Considering Costa Rica a particular case, 85% and 95.9% of the dairy and beef cattle are based on grazing farming, respectively [51]. Relatively favorable toxin profiles were still found in the tested samples. Thereby, surveillance efforts have been focused on compound feed. Generally speaking, ruminants are relatively less sensitive toward the effects of mycotoxins as rumen bacteria play a detoxification role [35,38]. For example, for DON (prevalence of 70.0% and 55.1% in beef cattle feed and dairy cattle feed, respectively), Charmley and collaborators determined that concentrations of 6000 μg kg⁻¹ neither affect feed intake nor are biotransferred to the milk [36,52].

3.2.3. Prevalence in Compound Feed destined for Poultry and Swine

Mycotoxin effects over monogastric animals are varied, depending on the species and physiological and productive stage [53]. For example, in pigs, fumonisin feed contamination is related to pulmonary, hepatic and cardiovascular lesions [54] while DON has been associated with a reduction of productive parameters and feed efficiency [54]. Besides, pigs are especially sensitive to ZEA, as it is directly related to reproductive disorders and low fertility rates [55]. Mycotoxin findings in poultry feed are also worrisome as birds are noticeably susceptible to molecules such as DON. For example, in broilers, trichothecene exposure (e.g., DON), through feed, increases mortality, reduces immune function, and impairs weight gain [56].

3.2.4. Prevalence in Pet Food

Mycotoxins in pet foods have already been reported by other countries, including industrialized ones (e.g., Portugal, USA, England, and Brazil) [57]. Mainly, Fusarium and Penicillium toxins have been described [51]. An elevated prevalence was described for DON and FB₁ (50.0% and 93.3%, respectively) [58]. Mycotoxicosis in pets is associated with chronic disease, liver and kidney damage, and cancer [58]. Finding mycotoxins in thermally treated foods is not uncommon as mycotoxins molecules can withstand relatively elevated temperature; low toxin reduction will occur during extrusion. Fungi colonization of pet extruded food is expected to be low as it possesses relatively low values of moisture and water activity [58,59]. Mycotoxin in pet foods may represent an additional burden to humans due to the pet closeness with their owners.

3.2.5. Prevalence in Fish Feed

Presence of mycotoxins in fish feed is another proof of an industry which has progressively substituted animal protein sources for vegetable ones [60,61]. In this regard, DON, OTA, and ZEA have been said to be responsible for weight loss, exacerbated feed conversion, and increased susceptibility to infection and disease in fish [61,62]. In line with the data reported herein, a recent report revealed that commercial fish feed samples were frequently contaminated with DON (i.e., over 80% of the samples) with mean concentrations of 289 μg kg⁻¹ [49]. Levels as low as 4.5 mg DON kg⁻¹ feed have already confirmed adverse effects in productive parameters and increased mortality in some fish. even in a relatively short period [62].

3.3. Geographical Distribution and Climate Influence for Fusarium Toxins Present in Animal Feed

A different spatial distribution profile was observed for AFM₁ and OTA, which are not produced by Fusarium species. Fusarium species have the potential of simultaneously producing the remainder of the toxins assayed [63,64]. OTA is a toxin produced by several fungal species including Aspergillus ochraceus, A. carbonarius, A. niger and Penicillium verrucosum [65]. On the other hand, AFM₁ is not only produced by Aspergillus species but it is also a product of metabolism [66]. Our data not only demonstrate that most sampling weight is centered on the Costa Rican Central Valley plateau, but the largest concentrations also occur therein (geographical zones with a high average relative humidity of 82%). The data also demonstrate that the intricate climate in tropical countries (such as Costa Rica) predicts the behavior of mycotoxin contamination as more challenging.

3.4. Aflatoxin M₁ in Liquid Milk

Milk is not only a staple commodity by itself, but it can accompany other potentially contaminated products (e.g., coffee, tea, or chocolate). Additionally, although AFM₁ is the most studied toxin in milk, other toxins have been described as well [67]. Other dairy products are derived from this raw material (e.g., cheese). Although processing is involved, these other dairy products can carry by themselves aflatoxin metabolites as well (see, for example, [68]). During 2017 alone, milk consumption was calculated to be 212 kg per capita [18]. Assuming the worst-case scenario (a sample with the highest concentration of 0.989 µg kg⁻¹), a Costa Rican citizen could be exposed up to 210 µg AFM₁ per year. Similarly, a Jersey calf weighing 25–30 kg at birth would be fed with 10% of its live weight with contaminated milk (from 2.5 to 3 kg of milk per day) [69]. Reiteratively, this means a daily exposure of 2.5–3 µg AFM₁ per day. Milk weaning can occur at ten weeks old [70]. Milk consumption level exposure is estimated to be 0.023 ng AFM₁ per kg body weight per day when a maximum level of 0.5 μg kg⁻¹ is used.

Much higher average concentrations of AFM₁ have been documented in other Latin-American countries [71]. Interestingly, AFB₁ (the parent compound of AFM₁) has been reported to be present in milk samples [71]). Besides the toxic burden that AFB₁ and AFM₁ have in the liver, recent evidence suggests that kidney toxicity is a certainty [66]. On the other hand, considerably low (i.e., 0.037 µg kg⁻¹) AFM₁ levels in milk have been recently reported, although prevalence rates are also relatively high (i.e., 38.8%), [71]. Other Latin-American countries have reported similar percentages [72,73,74,75], and recent prevalence studies have been published in industrialized countries [76,77,78,79]. Epidemiological studies [1] and risk assessment [80,81,82] are paramount to reduce mycotoxin exposure to both humans and animals.

Aflatoxin-contaminated feed must also be monitored to avoid feeding dairy cows with contaminated batches [83]. For instance, the association among most aflatoxin-contaminated feed ingredients and prevalence has been detailed [36,73]. Although the samples reported herein come from a highly industrialized sector, similar prevalence has been reported in fresh milk from small farms [84]. Consistent with our results, the seasonal distribution does not seem to affect AFM₁ prevalence [71], probably because Costa Rica has a tropical climate. In general, Costa Rica has relatively high temperatures (19–30°C), humidity (60–91%) and abundant rainfall (1400–4500 mm per year) during a great part of the year (i.e., two distinct seasons), in opposition to an Iranian study exhibited a lower prevalence of AFM₁ in bovine milk during spring [85]. Seasonal variations (i.e., during rainy season) were also described for milk from other species (i.e., sheep, goat, and camel) [81]. Other researchers have not documented a clear tendency regarding AFM₁ occurrence during seasons [73]. It has been suggested, however, that climate change can bear an impact on human exposure to aflatoxins and health [85]. Finally, the burden of AFM₁ exposure for a human can be twice as much as breast milk contamination, as has also been well documented [86]. Although some methods for reducing AFM₁ contamination are available [87], pre- and post-harvest strategies are still the most effective strategies [88].

4. Conclusions

Toxicologically relevant concentrations were found during the five-year survey as some sample concentrations exceeded the regulatory guidelines. Fumonisin and deoxynivalenol feed contamination is worrisome since these toxins have the capacity of being found in significant levels in these matrices, and, in our case, higher levels of toxins are found in the Central Valley of the country. Therefore, surveillance programs should be expanded to the outermost productive regions of the country to suppress sampling bias, if existing any. Thermopluvial conditions do not seem to have a considerable effect on toxin levels, although some metabolites actually seem to behave concurrently. Fusarium metabolites must be stridently monitored as it is clear that contamination in feed and feed ingredients is unfortunately common; this is especially true for fumonisins and T-2. Feed manufacturers, farmers (both in the field and storage facilities) and pet owners alike should be educated as to the proper conditions for food storage to avoid mycotoxin-producing fungal colonization. Toxin metabolite analysis and co-occurrence are paramount for complete surveillance of toxin feeds, and efficiently execute systems for the control and reduction of mycotoxins, as well as their metabolites in feeds. In addition, a strict control of AFM₁ in milk is necessary, because the prevalence of AFM₁ in milk is considerable and several samples exceeded the regulatory thresholds. It must be remembered that milk is the raw material for a wide variety of dairy products (butter, cheese, and yogurt, among others), therefore, the exposure of the population to this mycotoxin is increased.

5. Materials and Methods

5.1. Reagents

An analytical standard with certified concentrations, dissolved in acetonitrile, for DON, 3-ADON, T-2 (TSL-314), HT-2 (TSL-333), ZEA (TSL-401), FB₁, FB₂ (TSL-202), and OTA (TSL-504) was purchased from Trilogy^® Analytical Laboratory Inc (Washington, MO, USA). All standards have an initial concentration of 100 mg L⁻¹, except for FB₂ that was at 30 mg L⁻¹. Additionally, a naturally contaminated reference material (TRMT100, cornmeal) was used as a quality control sample (TS-108, Washington, MO, USA). Acetonitrile (ACN) and methanol (MeOH), both chromatographic grade, were purchased from J.T. Baker (Avantor Materials, Center Valley, PA, USA). Ultrapure water (type I, 0.055 µS cm⁻¹ at 25°C, 5 µg L⁻¹ TOC) was obtained using an A10 Milli-Q Advantage system and an Elix 35 system (Merck KGaA, Darmstadt, Germany).

5.2. Sampling

A total of n = 487 different feedstuffs of ca. 5 kg were collected during 2013–2017 by government inspectors from n = 107 Costa Rican feed manufacturers, as part of a countrywide surveillance program. Sample collection was composed of compound feed and feed ingredients, as follows: dairy cattle feed 28.9% (n = 141), cornmeal 9.9% (n = 48), citrus pulp 5.5% (n = 27), cattle feed 5.5% (n = 27), pig feed 5.3% (n = 26), calf feed 4.3% (n = 21), palm kernel meal 4.1% (n = 20), fish feed (Tilapia) 3.7% (n = 18), poultry feed 3.5% (n = 17), distillers dried grains 3.5% (n = 17), hay 3.3% (n = 16), dog food 3.3% (n = 16), wheat middlings 2.9% (n = 14), soybean meal 2.7% (n = 13), layer hen feed 2.0% (n = 10), horse feed 1.8% (n = 9), forage 1.8% (n = 7), pineapple byproducts 1.2% (n = 6), cassava meal 1.2% (n = 6), sorghum meal 0.6% (n = 3), rodent feed 0.6% (n = 3), ground roasted coffee 0.6% (n = 3), banana peel 0.6% (n = 3), rice bran 0.4% (n = 2), chamomile flowers 0.4% (n = 2), soybean hulls 0.2% (n = 1), shrimp feed 0.2% (n = 1), rice meal 0.2% (n = 1), rabbit feed 0.2% (n = 1), hydrolyzed feather meal 0.2% (n = 1), fish feed (snapper, n = 1), fish feed (salmon and trout, n = 1), corn silage (n = 1), and corn gluten (n = 1). Selection of feed and feed ingredients to be tested, number of samples, sampling sites, and specific toxins to assay (per matrix) were chosen by feed control officials. The selection considered the most common feedstuffs used in Costa Rica, import and export regulations, contamination risk factors, the productivity of the feed industry, and the risk for human and animal health associated with each feed or feed ingredient. Sampling was performed following the Association of American Feed Control Officials (AAFCO) recommendations for mycotoxin test object collection [89], and samples were taken from silos and storage reservoirs from feed manufacturing plants. All samples were quartered and sieved (1 mm particle size) [89]. Additionally, n = 180 dairy samples (mostly liquid bovine milk) from n = 13 different Costa Rican dairy farms were assayed; 50 mL subsamples were processed from 500 mL samples.

5.3. Reference Methods for Toxin Determination

Mycotoxins were assayed using the following methods: DON/3-ADON [90], T-2 and HT-2 toxins [91], ZEA AOAC 976.22, fumonisins AOAC 995.15, and OTA AOAC 991.44. AFM₁ was assayed according to the methods in [36,92] for milk and butter, respectively.

5.4. Chromatographic System and Conditions

All analytes were assayed using HPLC. Equipment consisted of an Agilent 1260 Infinity series HPLC with a quaternary pump (G1311B), a column compartment (G1316A), a variable wavelength and fluorescence detector (G1314B and G1321B) and an autosampler system (G1329A) (Agilent Technologies, Santa Clara, CA, USA). Peak separation was accomplished using a 5 mm Agilent Zorbax Eclipse C₁₈ column (3.0 × 150 mm, 5 µm) except for T-2/HT-2 toxin analyses for which a Luna^® Phenyl-Hexyl column (4.6 × 150 mm, 5 µm) was used (Phenomenex, Torrance, CA, USA). All analytes, except AFM₁, were extracted using Immunoaffinity columns (R-biopharm Rhöne Ltd, Darmstadt, Germany).

5.4.1. DON/3-ADON

DONPREP^® (R-biopharm) columns were used for sample extraction. Briefly, 200 mL of purified H₂O was added to 25 g of test portion. The mixture was dispersed using an Ultra-Turrax^® (T25, IKA Works GmbH & Co, Staufen, Germany) at 8000 rpm. The supernatant was filtered by gravity over an ashless filter paper (Grade 541, Whatman^®, GE Healthcare Life Sciences, Marlborough, MA, USA). Subsequently, an exact 2 mL aliquot from the supernatant was transferred to the IAC column and passed at 1 mL min⁻¹ using an SPE 12 port vacuum manifold (57044, Visiprep™, Supelco Inc., Bellefonte, PA, USA) at 15 mm Hg vacuum. After a washing step using 2× 10 mL water, the columns were left to dry and then four MeOH fractions of 500 µL were passed through the IAC. The total volume recovered was concentrated to dryness under vacuum at 60°C. The sample was reconstituted with MeOH to 300 µL and transferred to an analytical HPLC conical vial insert (5182-0549, Agilent Technologies, Santa Clara, CA, USA) before injection into the chromatograph.

Gradient mode starting at 80:20 H₂O, Solvent A/CH₃OH, Solvent B as per chromatographic conditions. The rest of the program was as follows: at 0.5 min 80% A, at 5.50 min 90% A, at 10 min 90% A, at 11 min 80% A, and at 15 min 80% A. DON and 3-ADON absorption at 220 nm was exploited for detection purposes. Linear calibration curves ranging from 1.25 to 10.00 µg mL⁻¹ were prepared during quantification. The limit of quantification for DON/3-ADON was 10.00 and 40.00 μg kg⁻¹.

5.4.2. T-2 and HT-2 Toxin

The extraction was similarly performed as detailed for DON/3ADON using an EASI-EXTRACT^® T-2 and HT-2 IAC (R-biopharm). Extraction solvent consisted in 125 mL of MeOH/H₂O (90:10) and 2.5 g of NaCl. An aliquot of 5 mL 10-fold diluted in PBS (1.37 mol L⁻¹) was passed through the column. Precolumn derivatization was performed after the evaporation step using 50 µL of 4-dimethylaminopyridine (107700, Sigma-Aldrich, St. Louis, Mo, USA) and 50 µL of 1-anthroyl cyanide (017-12101, FUJIFILM (Wako Pure Chemical Corporation, Osaka, Japan) both at 1 mg mL⁻¹ in toluene (TX0737, Sigma-Aldrich). Gradient mode started at 70:30 CH₃CN, Solvent A/H₂O, Solvent B as per chromatographic conditions. The rest of the program was as follows: at 5 min 70% A, at 15 min 70% A, at 25 min 85% A, at 27 min 100% A, at 32 min 100% A, and at 35 min 70% A. Flow rate was set at 1 mL min⁻¹. Adduct fluorescence was measured at λ_ex = 381 and λ_em = 470 nm. Linear calibration curves ranging from 125.00 to 1000.00 µg L⁻¹ were prepared during quantification. The limit of quantification for T-2 and HT-2, was 5.00 and 3.00 μg kg⁻¹, respectively.

5.4.3. ZEA

Extraction was performed using 100 mL of CH₃CN/H₂O 60:40 and an EASI-EXTRACT^® ZEARALENONE IAC (R-biopharm). Isocratic mode using a 40:10:50 CH₃CN/CH₃OH/H₂O mixture at a flow rate of 0.7 mL min⁻¹ was used as per chromatographic conditions. ZEA natural fluorescence (at λ_ex = 236, λ_em = 464 nm) was exploited for detection purposes. Linear calibration curves ranging from 300.00 to 1200.00 µg L⁻¹ were prepared during quantification. The limit of quantification was 0.072 μg kg⁻¹.

5.4.4. FB₁ and FB₂

Extraction was performed using 100 mL of CH₃CN/MeOH/H₂O (25:25:50) and FUMONIPREP^® IAC (R-biopharm). Fumonisin derivatization was based on the reaction with o-phthalaldehyde (Millipore Sigma, P0657) and 2-mercaptoethanol (Millipore Sigma, 97622) as stated on the reference method. However, pre-column derivatization was performed in situ in the autosampler injector, according to Bartolomeo and Maisano (2006), but increasing the sample and OPA reagent volume 5-fold. Adduct fluorescence was measured at λ_ex = 335 and λ_em = 440 nm. Isocratic mode using MeOH/0.1 mol L⁻¹ NaH₂PO₄ (77:23), adjusted to apparent pH 3.3 with H₃PO₄, was used at a 0.8 mL min⁻¹ flow rate. The limit of quantification was 0.05 μg kg⁻¹ for both FB₁ and FB₂.

5.4.5. OTA

Extraction was performed using 100 mL of CH₃CN/H₂O 60:40 and an OCRAPREP^® IAC column. OTA elution from column and resuspension after evaporation was achieved using a 98:2 MeOH and acetic acid solution to ensure OTA protonation. Isocratic mode using a 50:50 H₂O/CH₃CN mixture using 0.2 mol L⁻¹ trifluoroacetic acid, pH = 2.1 (74564 Millipore Sigma) at a flow rate of 0.7 mL min⁻¹ was used as per chromatographic conditions. OTA natural fluorescence (at λ_ex = 247, λ_em = 480 nm) was exploited for detection purposes. Linear calibration curves ranging from 2.50 to 40 µg L⁻¹ were prepared during quantification. The limit of quantification was 0.011 μg kg⁻¹.

5.4.6. AFM₁ in Milk and Butter

AflaStar^® M₁ (Romer Labs Diagnostic GmbH, Tulln an der Donau, Austria) columns were used for sample extraction. An exact 50 mL of raw or processed milk, previously homogenized and filtered by gravity over an ashless filter paper, was transferred to the IAC column. After a washing step using 3× 10 mL of water, the columns were left to dry and eluted using MeOH and concentrated as described above in 5.4.1. Isocratic mode using a 10:35:55 CH₃CN/CH₃OH/H₂O mixture at a flow rate of 0.6 mL min⁻¹ was used as per chromatographic conditions. AFM₁ natural fluorescence (at λ_ex = 365, λ_em = 455 nm) was exploited for detection purposes. Linear calibration curves ranging from 0.50 to 2.00 µg L⁻¹ were prepared during quantification. The limit of quantification was 0.014 μg kg⁻¹.

In the case of the butter samples, the preparation was performed according to the method in [84]. Briefly, 25 mL of aqueous methanol (70 mL/100 mL) was added to 5 g of butter. Afterwards, the solution was extracted by mixing gently for 10 min at room temperature using sonication. The extract was filtered through a paper filter, and 15 mL of distilled water was added to 5 mL of filtered solution. After that, 0.25 mL of Tween 20 were added and dispersed for 2 min, followed by the entire amount of the sample solution (20 mL) passing over the IAC.

5.5. Data Analysis

For Table 1, Table 2 and Table 3, prevalence is expressed as the ratio between the total of assays above the limit of detection and the total of assays performed for each toxin. Descriptive statistics displayed in Table 1 are expressed without considering samples below the limit of detection. Heat maps used in Figure 1 were rendered using ArcGIS Pro v2.2 (Esri^TM, Redlands, CA, USA). For each contaminant, Spearman Rank Order tests were applied to assess the association among the toxin concentration and climatic variables (i.e., precipitation, rainy days and temperature). In this particular case, toxin levels below the limit of detection were considered zero for association purposes; this analysis was performed using SigmaPlot 14 (Systat Software Inc., San Jose, CA, USA). Sampling date was linked to mean monthly values and data were retrieved from the closest climatological station to the sampling region. Meteorological data were provided by the Costa Rican National Weather Service (https://www.imn.ac.cr/boletin-meteorologico).

Author Contributions

Conceptualization, F.G.-C., and M.A.-C.; methodology, A.L., G.C., and F.G.-C.; validation, F.G.-C.; formal analysis, F.G.-C. and A.L.; resources, M.A.-C., G.C., and F.G.-C.; data curation, A.M. and F.G.-C.; writing—original draft preparation, A.M., A.L. and F.G.-C.; writing—review and editing, F.G.-C.; visualization, F.G.-C. and A.L.; supervision, F.G.-C.; project administration, M.A.-C. and G.C.; and funding acquisition, A.M.

Funding

The University of Costa Rica funded this research through grants ED-427 and ED-428, and the APC was supported by the Office of the Vice Provost for Research of the University of Costa Rica.

Acknowledgments

Geovanna Méndez is acknowledged for tabulating the data that corresponds to the year 2017. Special thanks to Mauricio Redondo-Solano and María Sabrina Sánchez for their suggestions, revising the manuscript and for language editing.

Conflicts of Interest

The authors declare no conflict of interest.

Appendix A

Table A1. Indicative Levels for T-2 and HT-2 in Cereals and Cereal products according to UE ^a.

Matrix	Indicative Levels for the Sum of T-2 and HT-2 (µg kg⁻¹) from Which Onwards/above Which Investigations Should be Performed, Certainly in Case of Repetitive Findings
Unprocessed Cereals
Barley (including malting barley) and maize	200
Oats (with husk)	1000
Wheat, rye and other cereals	100
Cereal Products for Feed and Compound Feed
Oat milling products (husks)	2000
Other cereal products	500
Compound feed with the exception of feed for cats	250

^a Based on Reference [38] and according to 2013/165/EU. Please see notes contained in each recommendation.

Table A2. Relevant guidance values for each mycotoxin in products intended for animal feed according to UE ^a.

Mycotoxin	Products Intended for Animal Feed	Guidance Value in mg kg⁻¹ Relative to a Feedstuff with a Moisture Content of 12 g/100 g
Deoxynivalenol	Feed materials
	Cereals and cereal products with the exception	8
	Cereals and cereal products with the exception	12
	Compound feed (exception of compound feed for pigs, calves (<4 months), lambs, kids and dogs)	5
	Compound feed for pigs	0.9
	Compound feed for calves (<4 months), lambs, kids and dogs	2
Zearalenone	Feed materials
	Cereals and cereal products with the exception of maize byproducts	2
	Maize byproducts	3
	Compound feed for:
	Piglets, gilts (young sows), puppies, kittens, dogs and cats for reproduction	0.1
	Adult dogs and cats other than for reproduction	0.2
	Sows and fattening pigs	0.25
	Calves, dairy cattle, sheep (including lamb) and goats (kids)	0.5
Ochratoxin A	Feed materials
	Cereals and cereal products	0.25
	Compound feed for
	Pigs	0.05
	Poultry	0.1
	Cats and dogs	0.01
Fumonisin FB₁ + FB₂	Feed materials
	Maize and maize products	60
	Compound feed for
	Pigs, horses (Equidae), rabbits and pet animals	5
	Fish
	Poultry, calves (<4 months), lambs and kids	20
	Adult ruminants (> 4 months) and mink	50
T2 + HT-2	Compound Feed for Cats	0.05

^a Based on Reference [37] and according to 2006/576/EC, 2016/1319, and definitions stated in 68/2013/EC. Please see notes contained in each recommendation.

References

Tola, M.; Kebede, B. Occurrence, Importance and Control of Mycotoxins: A Review. Cogent Food Agric. 2016. [Google Scholar] [CrossRef]
Lee, H.J.; Ryu, D. Worldwide occurrence of mycotoxins in cereals and cereal derived food products: Public Health Perspectives of their Co-occurrence. J. Agric. Food Chem. 2017, 65, 7034–7051. [Google Scholar] [CrossRef] [PubMed]
Arcella, D.; Gergelova, P.; Innocenti, M.L.; Steinkellner, H. Human and animal dietary exposure to T-2 and HT-2. EFSA J. 2017, 15, 4972. [Google Scholar]
Chen, S.S.; Li, Y.-H.; Lin, M.-F. Chronic Exposure to the Fusarium Mycotoxin Deoxynivalenol: Impact on Performance, Immune Organ, and Intestinal Integrity of Slow-Growing Chickens. Toxins 2017, 9, 334. [Google Scholar] [CrossRef] [PubMed]
Zhang, Y.; Han, J.; Zhu, C.-C.; Tang, F.; Cui, X.-S.; Kim, N.-H. Exposure to HT-2 toxins causes oxidative stress-induced apoptosis/autophagy in porcine oocytes. Sci. Rep. 2016, 6, 33904. [Google Scholar] [CrossRef]
Ismail, Z.; Basha, E.A.; Al-Nabulsi, F. Mycotoxins in animal feed, hazardous to both animals and human health. J. Vet. Med. Res. 2018, 5, 1145. [Google Scholar]
Bertero, A.; Moretti, A.; Spicer, L.J.; Caloni, F. Fusarium Molds and Mycotoxins: Potential Species-Specific Effects. Toxins 2018, 10, 244. [Google Scholar] [CrossRef] [PubMed]
Kinoshita, A.; Keese, C.; Meyer, U.; Starke, A.; Wrenzycki, C.; Dänicke, S.; Rehage, J. Chronic Effects of Fusarium Mycotoxins in rations with or without Increased Concentrate Proportion on the Insulin Sensitivity in Lactating Dairy Cows. Toxins 2018, 10, 188. [Google Scholar] [CrossRef] [PubMed]
Ashiq, S. Natural Occurrence of Mycotoxins in Food and Feed: Pakistan Perspective. Compr. Rev. Food Sci. Food Saf. 2015, 14, 159–175. [Google Scholar] [CrossRef]
Antonissen, G.; Van Immerseel, F.; Pasmans, F.; Ducatelle, R.; Haesebrouck, F.; Timbermont, L.; Verlinden, M.; Janssens, G.P.J.; Eeckhaut, V.; Eekhpout, M.; et al. The mycotoxin deoxynivalenol predisposes for the development of Clostridium perfringens-induced necrotic enteritis in broiler chickens. PLoS ONE 2014, 9, e108775. [Google Scholar] [CrossRef] [PubMed]
Streit, E.; Schatzmayr, G.; Tassis, P.; Tzika, E.; Marin, D.; Taranu, I.; Tabuc, C.; Nicoau, A.; Aprodu, I.; Puel, O.; et al. Current Situation of Mycotoxin Contamination and Co-occurrence in Animal Feed-Focus on Europe. Toxins 2012, 4, 788–809. [Google Scholar] [CrossRef] [PubMed]
Greco, M.; Pardo, A.; Pose, G. Mycotoxigenic Fungi and Natural Co-Occurrence of Mycotoxins in Rainbow Trout (Oncorhynchus mykiss) Feeds. Toxins 2015, 7, 4595–4609. [Google Scholar] [CrossRef] [PubMed]
Ismaeil, A.A.; Papenbrock, J. Mycotoxins: Producing Fungi and Mechanisms of Phytotoxicity. Agriculture 2015, 5, 492–537. [Google Scholar] [CrossRef] [Green Version]
Rostagno, H.; Teixeira, L.; Hannas, M.; Lopes, J.; Kazue, N.; Perazzo, F.; Saraiva, A.; Teixeira de Abreu, M.; Borges, P.; Flávia De Oliveira, R.; et al. Tablas Brasileñas Para aves y Cerdos, 4th ed.; Universidad Federal de Viçosa, Departamento de Zootecnia: Viçosa, Brasil, 2017; pp. 28–256. [Google Scholar]
De Blass, C.; Mateos, G.G.; García-Rebollar, P. Tablas FEDNA de Composición y Valor Nutritivo de Alimentos Para la Fabricación de Piensos Compuestos, 3rd ed.; Fundación Española para el Desarrollo de la Nutrición Animal: Madrid, Spain, 2010; Available online: http://www.fundacionfedna.org/ingredientes-para-piensos (accessed on 4 February 2019).
SEPSA [Secretaría Ejecutiva de Planificación Agropecuaria]. Informe de Comercio Exterior del Sector Agropecuario 2016–2017. 2018. Available online: http://www.sepsa.go.cr/docs/2018-004-Informe_Comercio_Exterior_Sector_Agropecuario_2016-2017.pdf (accessed on 4 February 2019).
Chacón, M. Evolución del Cultivo de Maíz en Costa Rica. Oficina Nacional de Semillas, 2017. Available online: http://ofinase.go.cr/certificacion-de-semillas/certificacion-de-semillas-de-maiz/evolucion-cultivo-maiz/ (accessed on 4 February 2019).
CIAB [Cámara de Industriales de Alimentos Balanceados]. Situación Actual de Alimentos Balanceados—Informe Anual. 2018. Available online: https://www.ciabcr.com/charlas/Nutrici%C3%B3n%20Animal%202018/Charlas/Carl_Oroz.pdf (accessed on 4 February 2019).
Karlovsky, P.; Suman, M.; Berthiller, F.; De Meester, J.; Eisenbrand, G.; Perrin, I.; Oswald, I.P.; Speijers, G.; Chiodini, A.; Recker, T.; et al. Impact of food processing and detoxification treatments on mycotoxin contamination. Mycotoxin Res. 2016, 32, 179. [Google Scholar] [CrossRef] [PubMed]
Ma, R.; Zhang, L.; Su, Y.-T.; Xie, W.-M.; Zhang, N.-Y.; Dai, J.-F.; Wang, Y.; Rajput, S.A.; Qi, D.-S.; Karrow, N.A.; et al. Individual and Combined Occurrence of Mycotoxins in Feed Ingredients and Complete Feeds in China. Toxins 2018, 10, 113. [Google Scholar] [CrossRef] [PubMed]
Chagwa, R.; Abia, W.; Msagati, T.; Nyoni, H.; Ndleve, K.; Njobeh, P. Multi-Mycotoxin Occurrence in Dairy Cattle Feeds from the Gauteng Province of South Africa: A Pilot Study Using UHPLC-QTOF-MS/MS. Toxins 2018, 10, 294. [Google Scholar] [CrossRef] [PubMed]
Franco, L.T.; Petta, T.; Rottinghaus, G.E.; Bordin, K.; Gomes, G.A.; Oliveira, C.A.F. Co-occurrence of mycotoxins in maize food and maize-based feed from small-scale farms in Brazil: A pilot study. Mycotoxin Res. 2018. [Google Scholar] [CrossRef]
Ul Hassan, Z.; Al Thani, R.; Atia, F.A.; Al Meer, S.; Migheli, Q.; Jaoua, S. Co-occurrence of mycotoxins in commercial formula milk and cereal-based baby food in Qatar. Food Addit. Contam. Part B 2018, 11, 191–197. [Google Scholar] [CrossRef] [PubMed]
Bozzo, G.; Bonerba, E.; Ceci, E.; Valeriana, C.; Tantillo, G. Determination of ochratoxin A in eggs and target tissues of experimentally drugged hens using HPLC–FLD. Food Chem. 2011, 126, 1278–1282. [Google Scholar]
Payros, D.; Alassane-Kpembi, I.; Pierron, A.; Loiseau, N.; Pinton, P.; Oswald, I.P. Toxicology for deoxynivalenol and its acetylated and modified forms. Arch. Toxicol. 2016, 90, 2931–2957. [Google Scholar] [CrossRef]
Munkvold, G.P.; Arias, S.; Taschl, I.; Gruber-Dorninger, C. Chapter 9: Mycotoxin in Corn: Occurrence, Impacts and Management. In Corn; AACC International Press: St. Paul, MN, USA, 2019; pp. 235–287. [Google Scholar] [CrossRef]
Guerre, P. Fusariotoxins in Avian Species: Toxicokinetics, Metabolism, and Persistence in Tissues. Toxins 2015, 7, 2289–2305. [Google Scholar] [CrossRef] [PubMed]
Alshannaq, A.; Yu, J.H. Occurrence, Toxicity, and Analysis of Major Mycotoxins in Food. Int. J. Envron. Res. Public Health 2017, 14, 632. [Google Scholar] [CrossRef] [PubMed]
Krska, R.; Sulyok, M.; Berthiller, F.; Schuhmacher, R. Mycotoxin testing: From Multi toxin analysis to metabolomics. JSM Mycotoxins 2017, 67, 11–16. [Google Scholar] [CrossRef]
Van der Fels-Klerx, H.J.; Adamse, P.; Punt, A.; van Asselt, E.D. Data analyses and modeling for risk-based monitoring of mycotoxins in animal feed. Toxins 2018, 10, 54. [Google Scholar] [CrossRef] [PubMed]
Streit, E.; Naehrer, K.; Rodrigues, I.; Schatzmayr, G. Mycotoxin occurrence in feed and feed raw materials worldwide: Long-term analysis with special focus on Europe and Asia. J. Sci. Food Agric. 2013, 93, 2892–2899. [Google Scholar] [CrossRef]
Pinotti, L.; Ottoboni, M.; Giormini, C.; Dell’Orto, V.; Cheli, F. Mycotoxin Contamination in the EU Feed Supply Chain: A Focus on Cereal Byproducts. Toxins 2016, 8, 45. [Google Scholar] [CrossRef]
Rodrigues, I.; Naehrer, K. A Thre-Year Survey on the Worldwide Occurrence of mycotoxins in feedstuff and feed. Toxins 2012, 4, 663–675. [Google Scholar] [CrossRef]
Selvaraj, J.N.; Wang, Y.; Zhou, L.; Zhao, Y.; Xing, F.; Dai, X.; Liu, Y. Recent mycotoxin survey data and advanced mycotoxin detection techniques reported from China: A review. Food Addit. Contam. Part A 2015, 32, 440–452. [Google Scholar] [CrossRef]
Granados-Chinchilla, F.; Molina, A.; Chavarría, G.; Alfaro-Cascante, M.; Bogantes, D.; Murillo-Williams, A. Aflatoxins occurrence through the food chain in Costa Rica: Applying the One Health approach to mycotoxin surveillance. Food Control 2017, 82, 217–226. [Google Scholar] [CrossRef]
Chavarría, G.; Granados-Chinchilla, F.; Alfaro-Cascante, M.; Molina, A. Detection of Aflatoxin Mn milk, cheese and sour cream samples from Costa Rica using enzyme-assisted extraction and HPLC. Food Addit. Contam. Part B 2015, 8, 128–135. [Google Scholar] [CrossRef]
EU Commission Recommendations (2006/576/EC) of 17 August 2006 on the presence of deoxynivalenol, zearalenone, ochratoxin A, T-2 and HT-2 and fumonisins in products intended for animal feeding. Off. J. Eur. Union 2016, 229, 7–9.
EU Commission Recommendations (2013/165/EU) of March 2013 on the presence of T-2 and HT-2 toxin in cereals and cereal products. Off. J. Eur. Union 2013, 91, 12–15.
FAO (Food and Agriculture Organization of the United Nations). Aquaculture Feed and Fertilizer Resources Information System. 2018. Available online: http://www.fao.org/fishery/affris/species-profiles/nile-tilapia/tables/en/ (accessed on 19 July 2018).
INRA. Equine Nutrition, 1st ed.; Wageningen Academic Publishers: Wageningen, The Netherlands, 2015; pp. 97–120. [Google Scholar]
FEDIAF. Nutritional Guidelines for Complete and Complementary Pet Food for Cats and Dog; FEDIAF: Brussels, Belgium, 2016; pp. 1–102. [Google Scholar]
Martínez Marín, A.L. Inclusion of feeds in pelleted concentrates intended for stall-fed leisure horses. Arch. Zootec 2008, 57, 115–122. [Google Scholar]
Milani, J.M. Ecological conditions affecting mycotoxin production in cereals: A review. Vet. Med. 2013, 58, 405–411. [Google Scholar] [CrossRef]
Wu, L.; Qiu, L.; Zhang, H.; Sun, J.; Hu, X.; Wang, B. Optimization for the Production of Deoxynivalenol and Zearalenone by Fusarium graminearum Using Response Surface Methodology. Toxins 2017, 9, 57. [Google Scholar] [CrossRef] [PubMed]
CODEX. Code of Practice for the Prevention and Reduction of Mycotoxin Contamination in Cereals; CAC/RCP 51-2003; CODEX: Rome, Italy, 2014. [Google Scholar]
Pettersson, H. Mycotoxin contamination of animal feed. In Woodhead Publishing Series in Food Science, Technology and Nutrition, Animal Feed Contamination; Fink-Gremmels, J., Ed.; Woodhead Publishing: Cambridge, UK, 2012; ISBN 9781845697259. [Google Scholar] [CrossRef]
Leiva, A.; Granados-Chinchilla, F.; Redondo-Solano, M.; Arrieta-González, M.; Pineda Salazar, E.; Molina, A. Characterization of the animal by-product meal industry in Costa Rica: Manufacturing practices through the production chain and food safety. Poult. Sci. 2018, 97, 2159–2169. [Google Scholar] [CrossRef] [PubMed]
U.S. Grains Council. 2017/2018 Corn Harvest Quality Report 2018. Available online: https://grains.org/corn_report/corn-harvest-quality-report-2017-2018/ (accessed on 4 February 2019).
Xie, S.; Zheng, L.; Wan, M.; Niu, J.; Liu, Y.; Tian, L. Effect of deoxynivalenol on growth performance, histological morphology, anti-oxidative ability and immune response of juvenile Pacific white shrimp, Litopenaeus vannamei. Fish Shellfish Immunol. 2018, 82, 442–452. [Google Scholar] [CrossRef]
Di Stefano, V. Occurrence & Risk of OTA Food and Feed in Food and Feed. Ref. Modul. Food Sci. 2018. [Google Scholar] [CrossRef]
INEC (Instituto Nacional de Estadística y Censos). Encuesta Nacional Agropecuaria 2017: Resultados Generales de las Actividades Ganaderas Vacuna y Porcina. 2019. Available online: http://www.inec.go.cr/multimedia/encuesta-nacional-agropecuaria-2017-datos-de-la-ganaderia-vacuna-y-porcina (accessed on 4 February 2019).
Charmley, E.; Trenholm, H.L.; Thompson, B.K.; Vudathala, D.; Nicholson, J.W.G.; Prelusky, B.D.; Charmley, L.L. Influence of level of deoxynivalenol in the diet of dairy cows on feed intake, milk production, and its composition. J. Dairy Sci. 1993, 76, 3580–3587. [Google Scholar] [CrossRef]
CAST. Mycotoxins: Risks in Plant, Animal, and Human Systems. Council for Agricultural Science and Technology; CAST: Ames, IA, USA, 2003; pp. 1–217. [Google Scholar]
Focht Müller, L.K.; Paiano, D.; Gugel, J.; Lorenzetti, W.R.; Morais Santurio, J.; de Castro Tavernari, F.; Micotti da Gloria, E.; Baldissera, M.D.; Da Silva, A.S. Post-weaning piglets fed with different levels of fungal mycotoxins and spray-dried porcine plasma have improved weight gain, feed intake and reduced diarrhea incidence. Microb. Pathog. 2018, 117, 259–264. [Google Scholar] [CrossRef]
Dänicke, S.; Winkler, J. Invited review: Diagnosis of zearalenone (ZEN) exposure of farm animals and transfer of its residues into edible tissues (carry over). Food Chem. Toxicol. 2015, 84, 225–249. [Google Scholar] [CrossRef] [PubMed]
Wang, A.; Hogan, N.S. Performance effects of feed-borne Fusarium mycotoxins on broiler chickens: Influences of timing and duration of exposure. Anim. Nutr. 2018, 5, 32–40. [Google Scholar] [CrossRef] [PubMed]
Boermans, H.J.; Leung, M.C.K. Mycotoxins and the pet food industry: Toxicological evidence and risk assessment. Int. J. Food Microbiol. 2007, 119, 95–102. [Google Scholar] [CrossRef] [PubMed]
Gazzotti, T.; Biagi, G.; Pagliuca, G.; Pinna, C.; Scardilli, M.; Grandi, M.; Zaghini, G. Occurrence of mycotoxins in extrude commercial dog food. Anim. Feed Sci. Technol. 2015, 202, 81–89. [Google Scholar] [CrossRef]
Atungulu, G.G.; Mohammadi-Shad, Z.; Wilson, S. Chapter 2—Mycotoxin Issues in Pet. In Food and Feed Safety Systems and Analysis; Academic Press: Cambridge, MA, USA, 2018; pp. 25–44. [Google Scholar]
García-Herranz, V.; Valdehita, A.; Navvas, J.M.; Fernández-Cruz, M.L. Cytotoxicity against fish and mammalian cells lines and endocrine activity of the mycotoxins beauvericin, deoxynivalenol and ochratoxin-A. Food Chem. Toxicol. 2019. [Google Scholar] [CrossRef]
Gonçalves, R.A.; Navarro-Guillén, C.; Gilannejad, N.; Días, J.; Schatzmayr, D.; Bichl, G.; Czabany, T.; Moyano, F.J.; Rema, P.; Yúfera, M.; et al. Impact of deoxynivalenol on rainbow trout: Growth performance, digestibility, key gene expression regulation and metabolism. Aquaculture 2018, 490, 362–372. [Google Scholar] [CrossRef] [Green Version]
Woźny, M.; Obremski, K.; Hliwa, P.; Gomulka, P.; Rożyński, R.; Wojtacha, P.; Florczyk, M.; Segner, H.; Brzuzan, P. Feed contamination with zearalenone promotes growth but affects the immune system of rainbow trout. Fish Shellfish Immunol. 2019, 84, 680–694. [Google Scholar]
Ferrigo, D.; Raiola, A.; Causin, R. Fusarium Toxins in Cereals: Occurrence, Legislation, Factors Promoting the Appearance and Their Management. Molecules 2016, 21, 627. [Google Scholar] [CrossRef]
Shi, W.; Tan, Y.; Wang, S.; Gardiner, D.M.; De Saeger, S.; Liao, Y.; Wang, C.; Fan, Y.; Wang, Z.; Wu, A. Mycotoxigenic Potentials of Fusarium Species in Various Culture Matrices Revealed by Mycotoxin Profiling. Toxins 2017, 9, 6. [Google Scholar] [CrossRef]
Malir, F.; Ostry, V.; Pfohl-Leszkowicz, A.; Malir, J.; Toman, J. Ochratoxin A: 50 Years of Research. Toxins 2016, 8, 191. [Google Scholar] [CrossRef]
Li, H.; Xing, L.; Zhang, M.; Wang, J.; Zheng, N. The Toxic Effects of Aflatoxin B1 and Aflatoxin M1 on Kidney through Regulating L-Proline and Downstream Apoptosis. BioMed Res. Int. 2018, 2018, 9074861. [Google Scholar] [CrossRef] [PubMed]
Becker-Algeri, T.A.; Castagnaro, D.; de Bartoli, K.; de Souza, C.; Drunkler, D.A.; Badiale-Furlong, E. Mycotoxins in Bovine Milk and Dairy Products: A Review. J. Food Sci. 2016, 81, R545–R552. [Google Scholar] [CrossRef] [PubMed]
Ramírez-Martínez, A.; Camarillo-Hernández, E.; Carvajal-Moreno, M.; Vargas-Ortiz, M.; Wesolek, N.; Del Carmen, G.; Jimenes, R.; García Alvarado, M.A.; Roudot, A.-C.; Salgado Cervantes, M.A.; et al. Assessment of Aflatoxin M1 and M2 exposure risk through Oaxaca cheese consumption in southeastern Mexico. Int. J. Environ. Health Res. 2018, 28, 202–213. [Google Scholar]
US Jersey. A Quality Heifer. Cornell University, 2008. Available online: https://www.usjersey.com/Portals/0/AJCA/2_Docs/QualityHeiferBrochure.pdf (accessed on 27 February 2019).
Franklin, S.T.; Amaral-Phillips, D.M.; Jackson, J.A.; Campbell, A.A. Health and performance of Holstein calves that suckled or were hand-fed colostrum and were fed one of three physical forms of starter. J. Dairy Sci. 2003, 86, 2145–2153. [Google Scholar] [CrossRef]
Scaglioni, P.T.; Becker-Algeri, T.; Drunkler, D.; Badiale-Furlong, E. Aflatoxin B1 and M1 in milk. Anal. Chim. Acta 2014, 829, 68–74. [Google Scholar] [CrossRef] [PubMed]
Michlig, N.; Signorini, M.; Gaggiotti, M.; Chiericatti, C.; Basílico, J.C.; Repetti, M.R.; Beldomenico, H.R. Risk factors associated with the presence of aflatoxin M1 in raw bulk milk from Argentina. Food Control 2016, 64, 151–156. [Google Scholar] [CrossRef]
Quevedo-Garza, P.A.; Amador-Espejo, G.G.; Cantú-Martínez, P.C.; Trujillo-Mesa, J.A. Aflatoxin M1 occurrence in fluid milk commercialized in Monterrey, Mexico. J. Food Saf. 2018, 38, e12507. [Google Scholar] [CrossRef]
Peña-Rodas, O.; Martinez-Lopez, R.; Hernandez-Rauda, R. Occurrence of Aflatoxin M1 in cow milk in El Salvador: Results from a two-year survey. Toxicol. Rep. 2018, 5, 671–678. [Google Scholar] [CrossRef]
Sibaja, K.V.; Gonçalves, K.D.M.; Garcia, S.D.O.; Feltrin, A.C.P.; Noguiera, W.V.; Badiale-Furlong, E.; Garda-Buffon, J. Aflatoxin M₁ and B₁ in Colombian milk powder and estimated risk exposure. Food Addit. Contam. Part B 2019, 30, 1–8. [Google Scholar] [CrossRef] [PubMed]
Iqbal, S.Z.; Jinap, S.; Pirouz, A.A.; Faizal, A.R.A. Aflatoxin M1 in milk and dairy products, occurrence and recent challenges: A review. Trends Food Sci. Technol. 2015, 46, 110–119. [Google Scholar] [CrossRef]
Yoon, B.R.; Hong, S.-Y.; Cho, S.M.; Lee, K.R.; Kim, M.; Chung, S.H. Aflatoxin M1 levels in dairy products from South Korea determined by high-performance liquid chromatography with fluorescence detection. J. Food Nutr. Res. 2016, 55, 171–180. [Google Scholar]
Assunçao, R.; Martins, C.; Viegas, S.; Viegas, C.; Jakobsen, L.S.; Pires, S.; Alvito, P. Climate change and the health impact of aflatoxins exposure in Portugal—An overview. Food Addit. Contam. Part A 2018, 35, 1610–1621. [Google Scholar] [CrossRef]
Bellio, A.; Bianchi, D.M.; Gramaglia, M.; Loria, A.; Nucera, D.; Gallina, S.; Gili, M.; Decastelli, L. Aflatoxin M1 in Cow’s Milk: Method Validation for Milk Sampled in Northern Italy. Toxins 2016, 8, 57. [Google Scholar] [CrossRef] [PubMed]
Gong, Y.Y.; Watson, S.; Routledge, M.N. Aflatoxin Exposure and Associated Human Health Effects, a Review of Epidemiological Studies. Food Saf. 2016, 4, 14–27. [Google Scholar] [CrossRef] [Green Version]
Milićević, D.R.; Spirić, D.; Radičević, T.; Velebit, B.; Steganović, S.; Milojević, L.; Janković, S. A review of the current situation of aflatoxin M1 in cow’s milk in Serbia: Risk assessment and regulatory aspects. Food Addit. Contam. Part A 2017, 34, 1617–1631. [Google Scholar] [CrossRef] [PubMed]
Ahlberg, S.; Grace, D.; Kiarie, G.; Kirino, Y.; Lindahl, J. A Risk Assessment of Aflatoxin M1 Exposure in Low Mid-Income Dairy Consumers in Kenya. Toxins 2018, 10, 348. [Google Scholar] [CrossRef] [PubMed]
Walte, H.-G.; Schwake-Anduschus, C.; Geisen, R.; Fritsche, J. Afaltoxin: Food chain transference from feed to food. J. Verbr. Lebensm 2016, 11, 295–297. [Google Scholar] [CrossRef]
Gonçalves, L.; Dalla Rosa, A.; Gonzales, S.L.; Feltes, M.M.C.; Badiale-Furlong, E.; Dors, G.C. Incidence of aflatoxin M1 in fresh milk from small farms. Food Sci. Technol. 2017, 37, 11–15. [Google Scholar] [Green Version]
Fallah, A.A.; Fazlollahi, R.; Emami, A. Seasonal study of aflatoxin M1 contamination in milk of four dairy species in Yazd, Iran. Food Control 2016, 68, 77–82. [Google Scholar] [CrossRef]
Cantú-Cornelio, F.; Aguilar-Toalá, J.E.; de León-Rodríguez, C.I.; Esparza-Romero, J.; Vallejo-Córdoba, A.F.; García, H.S.; Hernández-Mendoza, A. Occurrence and factors associated with the presence of aflatoxin M1 in breast milk samples of nursing mothers in central Mexico. Food Control 2016, 62, 16–22. [Google Scholar] [CrossRef]
Naeimipour, F.; Aghajani, J.; Kojuri, S.A.; Ayoubi, S. Useful Approaches for Reducing Aflatoxin M1 Content in Milk and Dairy Products. Biomed. Biotechnol. Res. J. 2018, 2, 94–99. [Google Scholar]
Udomkun, P.; Wiredu, A.N.; Nagle, M.; Müller, J.; Vanlauwe, B.; Bandyopadhyay, R. Innovative technologies to manage aflatoxins in foods and feeds and the profitability of application—A review. Food Control 2017, 76, 127–138. [Google Scholar] [CrossRef] [PubMed]
Association of American Feed Control Officials (AAFCO). Feed Inspector’s Manual, 7th ed.; AAFCO Inspection and Sampling Committee: Atlanta, GA, USA, 2017. [Google Scholar]
Czerwiecki, L.; Wilczyńska, G. Determination of Deoxynivalenol in cereal by HPLC-UV. Mycotoxin Res. 2003, 19, 31–34. [Google Scholar] [CrossRef]
Visconti, A.; Lattanzio, V.M.T.; Pascale, M.; Haidukowski, M. Analysis of T-2 and HT-2 toxins in cereal grains by immunoaffinity clean-up and liquid chromatography with fluorescence detection. J. Chromatogr. A 2005, 1075, 151–158. [Google Scholar] [CrossRef] [PubMed]
Aydemir Atasever, M.; Atasever, M.; Özturan, K.; Urçar, S. Determination of Aflatoxin M₁ level in Butter Samples Consumed in Erzurum, Turkey. Kafkas Univ. Vet. Fak. Derg. 2010, 16 (Suppl. A), S159–S162. [Google Scholar]

Figure 1. Heat map representing the geographical origin of samples and the mycotoxin concentration: (A) DON; (B) 3-ADON; (C) T-2 toxin; (D) HT-2 toxin; (E) ZEA; (F) FB₁; (G) FB₂; (H) OTA; and (I) AFM₁.

Figure 2. 3D mesh graphs representing the relationship among mycotoxin concentration, mean temperature, and sample date: (A) DON; (B) 3-ADON; (C) T-2 toxin; (D) HT-2 toxin; (E) ZEA; (F) FB₁; (G) FB₂; (H) OTA; and (I) AFM₁.

Table 1. Mycotoxin presence and concentration in animal feedstuff commercialized in Costa Rica.

Year	Sample Numbers, n						Prevalence (%) (Samples over the Limit of Detection)	Average ± Standard Deviation ^b	Median ^b
		Concentration Range, µg kg⁻¹ ^a					Concentration, µg kg⁻¹
		x < LoD	x < 250	250 ≤ x < 500	500 ≤ x < 1000	x ≥ 1000	Concentration, µg kg⁻¹
Zearalenone
2013	47	19	27	1	0	0	59.6	30 ± 80	10
2014	57	8	49	0	0	0	86.0	15 ± 15	11
2015	62	44	18	0	0	0	29.0	33 ± 62	7
2016	99	79	12	6	2	0	20.0	180 ± 225	44
2017	61	35	8	9	3	6	42.6	1055 ± 1587	392
Total	335	194	114	16	5	6	42.1	236 ± 784	18
3-acetyldeoxynivalenol
2015	67	53	7	3	2	2	20.9	1602± 4238	251
2016	91	74	7	0	3	7	18.7	1691 ± 2757	594
2017	54	40	7	3	3	1	25.9	400 ± 398	275
Deoxynivalenol
Total	212	167	21	6	8	10	21.2	1261 ± 2909	295
2013	40	11	0	7	10	12	72.5	10,439 ± 29,521	830
2014	87	18	15	32	10	12	79.3	966 ± 2442	372
2015	81	44	9	11	10	7	45.7	703 ± 916	467
2016	94	54	13	7	7	11	42.5	1150 ± 1888	355
2017	61	24	10	13	8	6	60.7	4147 ± 18,710	400
Total	363	151	47	70	45	48	58.4	2822 ± 13,805	439
		x < LoD	5 < x	10 ≤ x < 25	25 ≤ x < 50	x ≥ 50
Ochratoxin A
2013	49	41	8	0	0	0	16.3	2 ± 2	2
2014	101	59	42	0	0	0	41.6	1 ± 2	1
2015	64	15	49	0	0	0	76.7	11 ± 23	1
2016	95	68	26	0	0	1	28.4	90 ± 346	3
2017	50	30	20	0	0	0	40.0	32 ± 63	5
Total	360	214	145	0	0	0	40.6	25 ± 152	1
T-2 toxin
2013	48	23	12	8	1	4	52.1	406 ± 467	273
2014	126	49	56	15	6	0	61.1	171 ± 227	61
2015	91	66	24	0	1	0	27.5	39 ± 130	9
2016	93	77	15	0	0	1	17.2	180 ± 509	15
2017	47	36	11	0	0	0	23.4	20 ± 18	13
Total	406	251	119	23	8	5	47.0	177 ± 317	47
HT-2 toxin
2014	47	17	16	6	4	4	63.8	1113 ± 2661	217
2015	86	66	14	3	2	1	23.2	257 ± 399	151
2016	92	56	29	3	1	3	39.1	199 ± 359	53
2017	44	33	10	1	0	0	25.0	108 ± 71	103
Total	269	172	66	13	7	8	36.1	463 ± 1495	115
		x < LoD	x < 1250	1250 ≤ x < 2500	2500 ≤ x < 5000	x ≥ 5000
Fumonisin B₁
2013	31	29	1	0	1	0	6.4	1691 ± 2117	1670
2014	35	27	3	1	2	2	22.9	3814 ± 3793	3625
2015	24	10	6	2	1	5	58.3	4551 ± 5774	3865
2016	88	54	13	5	4	12	38.6	3468.48 ± 7159	740
2017	59	43	11	2	3	0	27.1	203.64 ± 48	230
Total	237	163	34	10	11	19	31.2	3390 ± 5505	3110
Fumonisin B₂
2014	8	0	4	0	0	4	100.0	2794 ± 2252	2830
2015	11	1	3	2	2	3	90.9	6635 ± 9404	2010
2016	33	21	10	0	0	2	36.4	9931 ± 18,380	1793
2017	29	24	5	0	0	0	17.2	866 ± 1131	175
Total	81	46	22	2	2	9	43.2	6353 ± 13,559	1560

^a Ranges based on guidance values for mycotoxins in animal feeds within the European Union (Commission Recommendations 2006/576/EC and 2013/165/EU). [37,38]. ^b Values are calculated based on the number of samples above limit of detection.

Table 2. Mycotoxin contamination levels for feed ingredients. ^a

Average ± Standard Deviation	Median	Sample Numbers above Guidance Value, n	Prevalence, % (Sample Totals Analyzed by Toxin) ^c
Concentration, µg kg⁻¹
Corn and Byproducts
Deoxynivalenol (12,000 µg kg⁻¹)^b
650 ± 346	440	0	61.1 (36)
Fumonisin B₁ (60,000 µg kg⁻¹ sum FB₁/FB₂)^b
18,280 ± 16,016	3230	0	35.9 (39)
HT-2 toxin (500 µg kg⁻¹ sum T-2/HT-2)^b
493 ± 927	84	3	62.6 (24)
Ochratoxin A (250 µg kg⁻¹)^b
18 ± 45	1	0	25.6 (39)
T-2 toxin
195 ± 256	53	3	55.7 (61)
Zearalenone (3000 µg kg⁻¹)^b
314 ± 895	15	2	71.1 (45)
Soybean Meal (there is no recommended Guidelines) ^b
Deoxynivalenol
188 ± 69	200	Not applicable	60.0 (5)
Fumonisin B₁
3045 ± 1096	3045	Not applicable	100.0 (2)
HT-2 toxin
5013 ± 6542	2140	Not applicable	50.0 (6)
T-2 toxin
120 ± 141	50	Not applicable	61.5 (13)
Wheat and Byproducts
Deoxynivalenol (8000 µg kg⁻¹)^b
20,290 ± 52,867	890	1	100.0 (8)
Fumonisin B₁ (60,000 µg kg⁻¹ sum FB₁/FB₂)^b
2050 ± 2234	576	0	50.0 (4)
HT-2 toxin (500 µg kg⁻¹ sum T-2/HT-2)^b
44 ± 50	65	0	66.7 (3)
Ochratoxin A (250 µg kg⁻¹)^b
2 ± 2	1	0	50.0 (4)
T-2 toxin
64 ± 61	54	0	75.0 (8)
Zearalenone (2000 µg kg⁻¹)^b
12 ± 14	5	0	28.6 (7)
Rice and Byproducts
3-acetyldeoxynivalenol (there is no recommended guideline)^c
351 ± 79	351	Not applicable	50.0 (4)
Deoxynivalenol (8000 µg kg⁻¹)^b
890 ± 400	1101	0	60.0 (5)
Palm Oil and Byproducts (there is no recommended guidelines) ^b
Deoxynivalenol
400 ± 359	286	Not applicable	55.6 (18)
T-2 toxin
330 ± 625	58	Not applicable	61.5 (13)
Zearalenone
19 ± 18	13	Not applicable	30.0 (10)
Fruit Pulps and Peels (there is no recommended guidelines) ^b
3-acetyldeoxynivalenol
2204 ± 2394	2104	Not applicable	40.0 (10)
Deoxynivalenol
21,249 ± 41,315	2160	Not applicable	50.0 (14)
Fumonisin B₁
16,564 ± 18,916	7010	Not applicable	34.7 (32)
Fumonisin B₂
10,100 ± 13,096	16,564	Not applicable	50.0 (4)
Ochratoxin A
4 ± 7	1	Not applicable	50.0 (12)
T-2 toxin
330 ± 464	50	Not applicable	13.3 (15)
Zearalenone
43 ± 31	21	Not applicable	11.8 (17)
Forages, Silages, and Hay (there is no recommended guidelines) ^b
3-acetyldeoxynivalenol
476 ± 431	335	Not applicable	54.5 (22)
Deoxynivalenol
655 ± 514	410	Not applicable	66.7 (30)
Fumonisin B₁
11,883 ± 6917	7740	Not applicable	9.4 (32)
Fumonisin B₂
3985 ± 5310	1020	Not applicable	22.2 (9)
HT-2 toxin
124 ± 132	126	Not applicable	25.0 (16)
Ochratoxin A
15 ± 30	2	Not applicable	54.5 (22)
T-2 toxin
119 ± 177	25	Not applicable	30.4 (23)
Zearalenone
314 ± 724	27	Not applicable	37.5 (24)
Others (there is no recommended guidelines) ^b
Deoxynivalenol
610 ± 519	567	Not applicable	38.5 (13)
Fumonisin B₁
4931 ± 5994	693	Not applicable	66.7 (3)
HT-2 toxin
193 ± 136	197	Not applicable	75.0 (12)
Ochratoxin A
1 ± 3	1	Not applicable	56.3 (64)
T-2 toxin
6 ± 3	6	Not applicable	38.5 (13)
Zearalenone
9 ± 5	9	Not applicable	18.2 (11)

^a Toxins detected only once for a specific matrix type were not included. ^b Data in parentheses indicate the permitted maximum or recommended toxin concentrations according to EU Commission Recommendations (2006/576/EC) [37] and (2013/165/EU) [34]. ^c Prevalence is calculated based on the number of samples above limit of detection.

Table 3. Mycotoxin contamination levels for compound animal feed. ^a

Average ± Standard Deviation	Median	Sample Numbers above Recommended Guidance Value, n	Prevalence, % (Sample Totals Analyzed by Toxin) ^d
Concentration, µg kg⁻¹
Beef Cattle Feed
3-acetyldeoxynivalenol (there is no recommended guideline)^c
166 ± 159	77	Not applicable	42.9 (7)
Deoxinivalenol (5000 µg kg⁻¹)^c
988 ± 1371	530	0	70.0 (10)
Fumonisin B₁ (50,000 µg kg⁻¹ sum FB₁/FB₂)^c
8912 ± 13,416	3305	0	88.9 (9)
Fumonisin B₂
4020 ± 4921	134	0	66.7 (3)
HT-2 toxin (250 µg kg⁻¹ sum T-2/HT-2^c
442 ± 736	20	1	37.5 (8)
T-2 toxin
128 ± 126	110	1	30.0 (10)
Ochratoxin A (there is no recommended guideline)^c
19 ± 22	12	Not applicable	44.4 (9)
Zearalenone (500 µg kg⁻¹)^c
269 ± 216	157	0	57.1 (7)
Ingredients ^b†: cornmeal (no restriction), soybean meal (no restriction), DDGG (12–15 g/100 g), palm kernel meal (max 10–15 g/100 g), wheat middlings (max 10–20 g/100 g), rice bran and polishings (max 10–20 g/100 g), soybean hulls (max 10 g/100 g), citrus pulp (10 g/100 g).
Dairy cattle Feed (Adults and Heifers)
3-acetyldeoxynivalenol (there is no recommended guideline)^c
1843 ± 4135	218	Not applicable	19.0 (105)
Deoxynivalenol (5000 µg kg⁻¹)^c
1578 ± 4613	338	5	55.1 (147)
Fumonisin B₁ (50,000 µg kg⁻¹ sum FB₁/FB₂^c
6171 ± 7908	1480	0	44.4 (144)
Fumonisin B₂
3838 ± 5913	2310	0	43.2 (44)
HT-2 toxin (250 µg kg⁻¹ sum T-2/HT-2^c
207 ± 282	106	13	35.6 (132)
Ochratoxin A (there is no recommended guideline^c
55 ± 259	1	Not applicable	35.0 (140)
T-2 toxin
184 ± 351	40	10	27.5 (171)
Zearalenone (500 µg kg⁻¹^c
215 ± 810	16	6	44.0 (150)
Ingredients ^b†: cornmeal (no restriction), soybean meal (no restriction), DDGG (12–15 g/100 g), palm kernel meal (max 10–15 g/100 g), wheat middlings (max 10–20 g/100 g), rice bran and polishings (max 10–20 g/100 g), soybean hulls (max 10 g/100 g), citrus pulp (10 g/100 g).
Poultry Feed
Deoxynivalenol (5000 µg kg⁻¹)^c
1550 ± 2327	405	2	71.4 (14)
Fumonisin B₁ (20,000 µg kg⁻¹ sum FB₁/FB₂)^c
17,147 ± 33,569	3860	1	70.0 (10)
Fumonisin B₂
436 ± 467	835	0	80.0 (5)
HT-2 toxin (250 µg kg⁻¹ sum T-2/HT-2)^c
353 ± 284	208	1	33.3 (15)
Ochratoxin A (100 µg kg⁻¹)^c
31 ± 48	11	1	44.4 (9)
T-2 toxin
316 ± 462	67	5	51.7 (29)
Zearalenone (there is no recommended guideline)^c
75 ± 117	28	Not applicable	50.0 (10)
Ingredients^b†: corn meal (no restriction), soybean meal (no restriction), DDGG (max 10–15 g/100 g), palm kernel meal (3–3.5 g/100 g), wheat middlings (max 3–3.5 g/100 g), rice bran and polishings (max 3–3.5 g/100 g), soybean hulls (max 3–3.5 g/100 g).
Pet Food (Cat and Dog Dry Food)
Deoxynivalenol (2000 µg kg⁻¹)^c
940 ± 1317	470	0	50.0 (14)
Fumonisin B1 (5000 µg kg⁻¹ sum FB1/FB2)^c
143,560 ± 479,783	3570	6	93.3 (15)
Ingredients ^b†: cornmeal (max 50 g/100 g), DDGG (max 25 g/100 g), palm kernel meal, wheat middlings (max 20 g/100 g), rice meal and bran (max 20 g/100 g).
Swine Feed (Lactating and Gestating Sows and Pig Grower)
Deoxynivalenol (900 µg kg⁻¹)^c
6302 ± 14,932	590	6	76.5 (17)
Fumonisin B₁ (5000 µg kg⁻¹ sum FB₁/FB₂)^c
20,042 ± 35,978	3124	2	55.6 (9)
Fumonisin B₂
376 ± 472	376	0	40.0 (5)
HT-2 toxin (250 µg kg⁻¹ sum T-2/HT-2)^c
3409 ± 4738	3409	1	28.6 (7)
T-2 toxin
183 ± 187	88	3	46.7 (15)
Zearalenone (100 µg kg⁻¹)^c
518 ± 1327	37	2	44.4 (18)
Ingredients ^b†: cornmeal (no restriction), soybean meal (no restriction), DDGG (max 10 g/100 g), palm kernel meal (max 10 g/100 g), wheat middlings (max 20–25 g/100 g), rice bran and polishing (max 20–25 g/100 g), soybean hulls (no restriction).
Fish Feed
Deoxynivalenol (500 µg kg⁻¹)^c
570 ± 318	635	2	25.0 (16)
Fumonisin B₁ (10,000 µg kg⁻¹ sum FB₁/FB₂)^c
10,851 ± 10,781	1565	2	52.4 (21)
Ochratoxin A (there is no recommended guideline)^c
3 ± 5	1	Not applicable	66.7 (24)
	T-2 toxin
4 ± 4	3	0	35.0 (20)
Zearalenone (there is no recommended guideline)^c
84 ± 122	35	Not applicable	25.0 (16)
Ingredients^b†: cornmeal (max 15 g/100 g), soybean meal (max 75 g/100 g), DDGG, palm kernel meal (max 30 g/100 g), wheat middlings (max 20 g/100 g), rice meal and bran (max 15 g/100 g), soybean hulls.
Horse Feed
Deoxynivalenol (5000 µg kg⁻¹)^c
740 ± 295	580	0	50.0 (6)
Fumonisin B₂
3355 ± 2623	3355	1	66.7 (3)
HT-2 toxin (250 µg kg⁻¹ sum T-2/HT-2)^c
52 ± 26	52	0	40.0 (5)
Ochratoxin A (there is no recommended guideline)^c
95.36 ± 47.43	95	Not applicable	33.3 (6)
T-2 toxin
49 ± 60	49	0	33.3 (6)
Ingredientsb†: cornmeal (max 45 g/100 g), soybean meal (max 13 g/100 g), DDGG (max 20 g/100 g), palm kernel meal, wheat middlings (max 25 g/100 g), rice bran, soybean hulls (max 20 g/100 g).

^a Toxins detected only once for a specific matrix type were not included. ^b Plant-derived constituents according to guaranteed labels. Data in parentheses indicate maximum inclusion recommended for each ingredient during feed formulation. ^† Data compiled from [15,39,40,41,42]. ^c Data in parentheses indicate maximum permitted or recommended toxin concentrations according to EU Commission Recommendations (2006/576/EC) [37] and (2013/165/EU) [38]. ^d Prevalence is calculated considering the number of samples above limit of detection.

Table 4. Seasonal prevalence and behavior per toxin.

Concentration, mg kg⁻¹
Season ^a	Positive Samples, n (Prevalence, %)	Average ± SD	Maximum
3-ADON
Rainy Season	36/145 (24.8)	2 ± 3	16
DON
Dry Season	57/101 (56.4)	3 ± 7	52
Rainy Season	130/229 (56.8)	17 ± 161	1830
FB₁
Dry Season	29/97 (29.9)	7 ± 12	40
Rainy Season	111/226 (49.1)	7 ± 13	77
FB₂
Dry Season	9/21 (42.9)	4 ± 8	23
Rainy Season	25/56 (44.6)	3 ± 4	19
HT-2 toxin
Rainy Season	96/180 (53.3)	1 ± 2	11
T-2 toxin
Dry Season	54/145 (37.2)	< 1	2
Rainy Season	94/248 (37.9)	< 1	1
OTA, μg kg⁻¹
Dry Season	31/112 (27.7)	7 ± 24	137
Rainy Season	88/204 (43.1)	37 ± 193	1810
ZEA
Dry Season	46/94 (48.9)	1 ± 1	6
Rainy Season	90/228 (39.5)	1 ± 6	4
Overall Months with Higher Levels and Prevalence
3-ADON	April and May	DON	No clear distribution
FB₁	June, July, and September	FB₂	April, June, and September
HT-2 toxin	October and November	T-2 toxin	No clear distribution
OTA	May and September	ZEA	May, July, and October

^a Dry season and rainy season defined as per mean precipitation, the former exemplified by the months between December and April where x < 80 mm rain.

Table 5. Mycotoxin co-occurrence in the sample totals.

Number of Toxins Simultaneously Present	2	3		4		5		6	7
Samples, n (Incidence, %)	141/279 ^a (50.54)	81/279 (29.0)		36/279 (12.9)		17/279 (6.1)		1/279 (0.4)	3/279 (1.1)
Toxin/Metabolite	Sample Numbers with the toxin present, n		Co-occurrence, n				Incidence, %
DON/3-ADON	177		18				10.2
FB₁/FB₂	137		23				16.8
T-2/HT-2 toxin	155		66				42.6
Toxin Co-occurrence with OTA	Sample Numbers, n				Incidence, %
DON + HT-2 toxin + ZEA	1				1.0
DON + 3-ADON + FB₁ + ZEA	1				1.0
T-2 toxin + FB₁ + ZEA	1				1.0
DON + FB1 + FB2 + ZEA	1				1.0
3-ADON	1				1.0
DON + 3-ADON + T-2 toxin + FB₁	1				1.0
DON + HT-2 toxin + FB₁ + ZEA	2				2.0
T-2 toxin + HT-2 toxin + FB₁ + ZEA	2				2.0
DON + 3-ADON + T-2 toxin + HT-2 T-2 toxin + FB_{1 +} FB₂ + ZEA	2				2.0
FB1 + ZEA	2				2.0
T-2/HT-2 toxin + ZEA	3				2.9
T-2 toxin + FB₁	3				2.9
DON + T-2 toxin + HT-2 toxin	4				3.9
DON + ZEA	4				3.9
DON + T-2 + FB₁ + ZEA	6				5.9
DON	7				6.9
HT-2 toxin	8				7.8
T-2 toxin	10				9.8
FB₁/FB₂	12				11.8
DON + FB₁	14				13.7
ZEA	17				16.7

^a Corresponds to the total number of samples in which ≥ 2 simultaneous toxins occurred.

Table 6. Prevalence and epidemiological data regarding AFM₁ in fresh bovine milk for four years.

Concentration ^b, ng mL⁻¹
Year	Positive Samples, n (Prevalence, %) ^a	Samples > 0.05 µg kg−¹, n (%)	Samples > 0.5 µg kg⁻¹, n (%)	Average ± SD	Median	Maximum	Minimum
2017	24/38 (63.2)	16 (42.1)	0	0.083 ± 0.076	0.061	0.334	0.013
2016	8/27 (29.6)	2 (7.4)	0	0.042 ± 0.030	0.032	0.109	0.014
2015	34/73 (46.6)	16 (21.9)	3 (4.1)	0.154 ± 0.236	0.057	0.989	0.017
2014	32/45 (71.1)	11 (24.4)	0	0.042 ± 0.038	0.030	0.164	0.005
Overall	98/183 (53.5)	45 (45.9)	3 (3.1)	0.091 ± 0.155	0.049	0.989	0.005
Dry season ^c	28/45 (62.2)	14 (50.0)	0	0.075 ± 0.105	0.050	0.485	0.005
Rainy season ^c	69/138 (50.0)	34 (49.3)	3 (4.3)	0.098 ± 0.172	0.049	0.989	0.005
Overall months with higher levels and prevalence				March, August, and September

^a Prevalence understood as the number of samples > Limit of quantificaction of 0.014 µg kg⁻¹. ^b Values obtained using only positive samples, i.e., > limit of detection. ^c Dry season and rainy season defined as per mean precipitation, the former defined by the months between December and April where x < 80 mm rain.

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Molina, A.; Chavarría, G.; Alfaro-Cascante, M.; Leiva, A.; Granados-Chinchilla, F. Mycotoxins at the Start of the Food Chain in Costa Rica: Analysis of Six Fusarium Toxins and Ochratoxin A between 2013 and 2017 in Animal Feed and Aflatoxin M₁ in Dairy Products. Toxins 2019, 11, 312. https://doi.org/10.3390/toxins11060312

AMA Style

Molina A, Chavarría G, Alfaro-Cascante M, Leiva A, Granados-Chinchilla F. Mycotoxins at the Start of the Food Chain in Costa Rica: Analysis of Six Fusarium Toxins and Ochratoxin A between 2013 and 2017 in Animal Feed and Aflatoxin M₁ in Dairy Products. Toxins. 2019; 11(6):312. https://doi.org/10.3390/toxins11060312

Chicago/Turabian Style

Molina, Andrea, Guadalupe Chavarría, Margarita Alfaro-Cascante, Astrid Leiva, and Fabio Granados-Chinchilla. 2019. "Mycotoxins at the Start of the Food Chain in Costa Rica: Analysis of Six Fusarium Toxins and Ochratoxin A between 2013 and 2017 in Animal Feed and Aflatoxin M₁ in Dairy Products" Toxins 11, no. 6: 312. https://doi.org/10.3390/toxins11060312

APA Style

Molina, A., Chavarría, G., Alfaro-Cascante, M., Leiva, A., & Granados-Chinchilla, F. (2019). Mycotoxins at the Start of the Food Chain in Costa Rica: Analysis of Six Fusarium Toxins and Ochratoxin A between 2013 and 2017 in Animal Feed and Aflatoxin M₁ in Dairy Products. Toxins, 11(6), 312. https://doi.org/10.3390/toxins11060312

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