αD-Conotoxins in Species of the Eastern Pacific: The Case of Conus princeps from Mexico
Abstract
:1. Introduction
2. Results
2.1. Peptidic Fractions of the C. princeps Venom and Determination of Activity on nAChRs
2.2. Purification of an Active Peptide of C. princeps Venom and Determination of Activity on nAChRs
2.3. Molecular Mass and Amino Acid Sequence of the Purified Peptide
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Obtainment of Crude Venom Extract
4.3. Fractionation of the Crude Venom Extract
4.4. Purification of the Active Peptide
4.5. Determination of Electrophysiological Activity on nAChRs
4.6. Determination of Molecular Mass and Amino Acid Sequence of the Purified Peptide
4.7. Similarity Search and Sequence Alignment
4.8. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Hernández-Sámano, A.C.; Falcón, A.; Zamudio, F.; Batista, C.V.F.; Michel-Morfín, J.E.; Landa-Jaime, V.; López-Vera, E.; Jeziorski, M.C.; Aguilar, M.B. αD-Conotoxins in Species of the Eastern Pacific: The Case of Conus princeps from Mexico. Toxins 2019, 11, 405. https://doi.org/10.3390/toxins11070405
Hernández-Sámano AC, Falcón A, Zamudio F, Batista CVF, Michel-Morfín JE, Landa-Jaime V, López-Vera E, Jeziorski MC, Aguilar MB. αD-Conotoxins in Species of the Eastern Pacific: The Case of Conus princeps from Mexico. Toxins. 2019; 11(7):405. https://doi.org/10.3390/toxins11070405
Chicago/Turabian StyleHernández-Sámano, Arisaí C., Andrés Falcón, Fernando Zamudio, César V.F. Batista, Jesús Emilio Michel-Morfín, Víctor Landa-Jaime, Estuardo López-Vera, Michael C. Jeziorski, and Manuel B. Aguilar. 2019. "αD-Conotoxins in Species of the Eastern Pacific: The Case of Conus princeps from Mexico" Toxins 11, no. 7: 405. https://doi.org/10.3390/toxins11070405