Prevalence and Genetic Diversity of Staphylococcal Enterotoxin (-Like) Genes sey, selw, selx, selz, sel26 and sel27 in Community-Acquired Methicillin-Resistant Staphylococcus aureus
Abstract
:1. Introduction
2. Results
2.1. The Prevalence of sey, selw, selx, selz, sel26, and sel27
2.2. Phylogenetic and Sequence Analysis of sey, selw, selx, and selz
3. Discussion
4. Materials and Methods
4.1. CA-MRSA Isolates
4.2. Genetic Analysis of S. aureus (CA-MRSA) Isolates
4.3. GenBank Accession Numbers
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Genotype | Total No. of Isolates | No. of Isolates With SE(-Like) Gene *1 (%) | ||||
---|---|---|---|---|---|---|
coa Genotype | spa Type (n = 149) *2 | sey | selw | selx | selz | |
Ia | t375 (3) | 17 | 8 (47.1) | 8 (47.1) | 13 (76.5) | 12 (70.6) |
IIa | t002 (56), t548 (2), t2487 (2), t001 (1), t045 (2) | 455 | 157 (34.5) | 450 (98.9) | 397 (87.3) | 5 (1.1) |
IIIa | t008 (9), t4133 (2), t1767 (24), t5071 (1), t1627 (2), t1581(2) | 74 | 22 (29.7) | 64 (86.5) | 60 (81.1) | 0 |
IVa | t019 (1) | 3 | 0 | 1 (33.3) | 0 | 0 |
Va | t5110 (6), t10641 (10) | 16 | 13 (81.3) | 8 (50) | 16 (100) | 14 (87.5) |
Vb | NT (1) | 1 | 0 | 1 (100) | 1 (100) | 0 |
VIa | ND | 4 | 0 | 0 | 0 | 0 |
VIIa | t1784 (23) | 51 | 20 (39.2) | 46 (90.2) | 46 (90.2) | 4 (7.8) |
VIIb | t370 (2) | 3 | 1 (33.3) | 2 (66.7) | 1 (33.3) | 0 |
Total | 624 | 221 (35.4) | 580 (92.9) | 534 (85.6) | 35 (5.6) | |
SCCmec type | ||||||
SCCmec I | 2 | 0 | 1 (50) | 2 (100) | 0 | |
SCCmec II | 452 | 154 (34.1) | 431 (95.4) | 393 (86.9) | 5 (1.1) | |
SCCmec IV | 125 | 41 (32.8) | 115 (92) | 106 (84.8) | 4 (3.2) | |
SCCmec V | 34 | 26 (76.5) | 30 (88.2) | 30 (88.2) | 26 (76.5) | |
SCCmec NT | 11 | 0 | 3 (27.3) | 3 (27.3) | 0 | |
Specimen | ||||||
sputum | 136 | 45 (33.1) | 130 (95.6) | 121 (89) | 9 (6.6) | |
urine | 129 | 40 (31) | 118 (91.5) | 102 (79.1) | 3 (2.3) | |
ear discharge | 76 | 35 (46.1) | 72 (94.7) | 70 (92.1) | 6 (7.9) | |
nasal discharge | 75 | 28 (37.3) | 73 (97.3) | 67 (89.3) | 7 (9.3) | |
pus | 57 | 24 (42.1) | 54 (94.7) | 52 (91.2) | 3 (5.3) | |
wound swab | 29 | 13 (44.8) | 27 (93.1) | 27 (93.1) | 3 (10.3) | |
eye swab | 29 | 12 (41.4) | 28 (96.6) | 26 (89.7) | 2 (6.9) | |
stool | 33 | 9 (27.3) | 26 (78.8) | 24 (72.7) | 0 | |
skin | 26 | 10 (38.5) | 24 (92.3) | 23 (88.5) | 2 (7.7) | |
Others *3 | 34 | 5 (14.7) | 28 (82.4) | 22 (64.7) | 0 |
PVL/ACME Genes | ST (CC) | Total No. of Isolates (n = 100) | SE(-Like) Genes Identified *2 |
---|---|---|---|
PVL+/ACME+ | ST8 (CC8) | 9 *1 | sek, seq, selw, selx |
PVL+/ACME- | ST30 (CC30) | 1 *1 | sem, sen, seo, seu, selw, selx |
ST59 (CC59) | 1 *1 | seb, sek, seq, selw, selx | |
PVL-/ACME+ | ST5/ST764 (CC5) | 15 *1 | seb (67%), sec (20%), seg, sei, sem, sen, seo, seu, sep (33%), selw, selx, sey |
PVL-/ACME- | ST8 (CC8) (CA-MRSA/J *3) | 5 *1 | sec, sel, sep, selw, selx |
ST8 (CC8) | 14 | selj (29%), ser (29%), selw (93%), selx (93%), sey (43%) | |
ST5/ST764 (CC5) | 20 | seb (60%), sec (10%), seg, sei, sem, sen, seo, seu, selw, selx, sey (30%), selz (25%) | |
ST5425 (CC5) | 1 | seg, sei, sem, sen, seo, selw, selx | |
ST45 (CC45) | 2 | seg, sei, sem, sen, seo, selw, selx, sey | |
ST1 (CC1) | 13 | sea, sek, seq, selx (92%), selw (92%), sey (31%), selz (15%) | |
ST89 (CC89) | 8 | sem, seo, seu, selw, selx, sey, selz | |
ST121 (CC121) | 10 | seg, sei, sem, sen, seo, seu, selw (50%), selx, sey (80%), selz (85%) | |
ST12 (CC12) | 1 | sep, selw, selx, selz |
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Aung, M.S.; Urushibara, N.; Kawaguchiya, M.; Ito, M.; Habadera, S.; Kobayashi, N. Prevalence and Genetic Diversity of Staphylococcal Enterotoxin (-Like) Genes sey, selw, selx, selz, sel26 and sel27 in Community-Acquired Methicillin-Resistant Staphylococcus aureus. Toxins 2020, 12, 347. https://doi.org/10.3390/toxins12050347
Aung MS, Urushibara N, Kawaguchiya M, Ito M, Habadera S, Kobayashi N. Prevalence and Genetic Diversity of Staphylococcal Enterotoxin (-Like) Genes sey, selw, selx, selz, sel26 and sel27 in Community-Acquired Methicillin-Resistant Staphylococcus aureus. Toxins. 2020; 12(5):347. https://doi.org/10.3390/toxins12050347
Chicago/Turabian StyleAung, Meiji Soe, Noriko Urushibara, Mitsuyo Kawaguchiya, Masahiko Ito, Satoshi Habadera, and Nobumichi Kobayashi. 2020. "Prevalence and Genetic Diversity of Staphylococcal Enterotoxin (-Like) Genes sey, selw, selx, selz, sel26 and sel27 in Community-Acquired Methicillin-Resistant Staphylococcus aureus" Toxins 12, no. 5: 347. https://doi.org/10.3390/toxins12050347