Next Article in Journal
Can (We Make) Bacillus thuringiensis Crystallize More Than Its Toxins?
Previous Article in Journal
Assessment of Mycotoxin Exposure in a Rural County of Chile by Urinary Biomarker Determination
Previous Article in Special Issue
A Review on Mycotoxins and Microfungi in Spices in the Light of the Last Five Years
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Toxins
Volume 13
Issue 7
10.3390/toxins13070440
toxins-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

The Presence of Aflatoxin M1 in Milk and Milk Products in Bangladesh

by
Abu Hasan Sumon
1,
Farjana Islam
1,
Nayan Chandra Mohanto
1,
Rahanuma Raihanu Kathak
1,
Noyan Hossain Molla
1,
Sohel Rana
2,
Gisela H. Degen
3,† and
Nurshad Ali
1,*
1
Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet 3114, Bangladesh
2
Department of Veterinary and Animal Science, Rajshahi University, Rajshahi 6205, Bangladesh
3
Leibniz-Research Centre for Working Environment and Human Factors (IfADo) at the TU Dortmund, Ardeystr. 67, D-44139 Dortmund, Germany
*
Author to whom correspondence should be addressed.
†
shared senior authorship.
Toxins 2021, 13(7), 440; https://doi.org/10.3390/toxins13070440
Submission received: 6 June 2021 / Revised: 20 June 2021 / Accepted: 23 June 2021 / Published: 25 June 2021
(This article belongs to the Special Issue Exposure to Mycotoxins via Food Chain)
Review Reports Versions Notes

Abstract

:
As milk provides both micro- and macronutrients, it is an important component in the diet. However, the presence of aflatoxin B1 (AFB1) in the feed of dairy cattle results in contamination of milk and dairy products with aflatoxin M1 (AFM1), a toxic metabolite of the carcinogenic mycotoxin. With the aim to determine AFM1 concentrations in milk and milk products consumed in Bangladesh, in total, 145 samples were collected in four divisional regions (Sylhet, Dhaka, Chittagong, and Rajshahi). The samples comprised these categories: raw milk (n = 105), pasteurized milk (n = 15), ultra-high temperature (UHT)-treated milk (n = 15), fermented milk products such as yogurt (n = 5), and milk powder (n = 5). AFM1 levels in these samples were determined through competitive enzyme-linked immunosorbent assay (ELISA). Overall, AFM1 was present in 78.6% of milk and milk products in the range of 5.0 to 198.7 ng/L. AFM1 was detected in 71.4% of raw milk (mean 41.1, range 5.0–198.7 ng/L), and in all pasteurized milk (mean 106, range 17.2–187.7 ng/L) and UHT milk (mean 73, range 12.2–146.9 ng/L) samples. Lower AFM1 levels were found in yogurt (mean 16.9, range 8.3–41.1 ng/L) and milk powder samples (mean 6.6, range 5.9–7.0 ng/L). About one-third of the raw, pasteurized, and UHT milk samples exceeded the EU regulatory limit (50 ng/L) for AFM1 in milk, while AFM1 levels in yogurt and milk powder samples were well below this limit. Regarding regions, lower AFM1 contamination was observed in Chittagong (mean 6.6, max 10.6 ng/L), compared to Sylhet (mean 53.7, max 198.7 ng/L), Dhaka (mean 37.8, max 97.2 ng/L), and Rajshahi (mean 34.8, max 131.4 ng/L). Yet, no significant difference was observed in AFM1 levels between summer and winter season. In conclusion, the observed frequency and levels of aflatoxin contamination raise concern and must encourage further monitoring of AFM1 in milk and milk products in Bangladesh.
Keywords:
aflatoxin M1; Bangladesh; ELISA; milk; milk products
Key Contribution: This study found that AFM1 is frequently present in milk and milk products in Bangladesh and about one-third of the raw, pasteurized, and UHT milk samples exceeded the EU regulatory limit.

1. Introduction

Milk is an important food as it provides micro- and macronutrients essential for the growth and maintenance of human health [1]. As milk and milk products are consumed by all age groups, including young children, dairy milk must be free of toxic compounds, including mycotoxins [2]. However, milk and milk-based products can contain aflatoxin M1 (AFM1), a metabolite of the mycotoxin aflatoxin B1 (AFB1), a potent human carcinogen [3]. AFM1 occurs in the milk of dairy cattle ingesting feed contaminated with AFB1, which is partly converted to this hydroxylated metabolite and then excreted in milk. The fraction of AFB1 in feed that is transferred to milk as AFM1 (carry-over) ranges between 0.6% and 3% with up to 6.2% in high yielding cows [4,5,6]. Due to its strong toxicity in many species, AFB1 is an undesirable substance in animal feed [7], and its levels in feed for dairy cattle are restricted in several countries in order to minimize carry-over and thereby human AFM1 exposure with milk [1,8].
The presence of AFM1 in milk and dairy products represents a worldwide concern for several reasons: (i) although AFM1 is known to exert lower carcinogenic potency than AFB1, their acute toxicities are rather similar [1,9,10]; (ii) AFM1 is heat stable, and normal processing and storage are not effective in reducing its levels in milk and milk products [11,12]; and (iii) small levels of this contaminant may impose health risks for consumers of large quantities of milk products, such as children, a particularly vulnerable subgroup in the population [13,14].
To protect humans against adverse effects from mycotoxin exposure, regulatory standards (limit values) for aflatoxins as contaminants in food and feed have been set in many countries. For instance, the European Commission (EC) established a maximum limit (ML) of 50 ng of AFM1/kg in milk, and a limit for AFM1 of 25 ng/kg in infant formula and follow-up formula [15], while other regulatory authorities and the Codex Alimentarius Commission have set a higher limit of 500 ng/kg in raw milk [16].
Aflatoxin contamination of feed commodities is frequent in some regions of the world, including South and Southeast Asia [17,18]. Bangladesh has a tropical monsoon climate, with clear seasonal variations in rainfall, temperature, and humidity; agriculture is highly dependent on the weather, with crop production mainly in summer and winter [19,20]. As high humidity at harvest and in storage favor the growth of aflatoxin-producing fungi, it is expected that crops in Bangladesh are also affected. Indeed, when aflatoxins were analyzed in cereal samples collected in all Bangladeshi districts and six times during a year, maize had the highest prevalence and level of contamination; contaminant levels were lower, yet still significant, in rice and in wheat, and all samples showed considerable seasonal variability [21]. Another study in Bangladesh reported the presence of AFB1 in pooled samples of commonly consumed food commodities and in poultry feed [22]. However, data on mycotoxins are scarce, in particular in feeds for livestock, a major part of the economy of Bangladesh [23].
For developing countries, where regulation on food and feed contaminants is not in place or not enforced by regular surveillance, monitoring of AFM1 occurrence in milk is recommended, as it is easy to perform and an indicator for dairy feed contamination. Furthermore, as milk and its products are important components of the human diet, the presence of AFM1 in these products may impose health risks for the consumers. This view is supported by a recent, first study on AFM1 contamination of raw milk and processed milk samples, collected at regional small farms and grocery stores in the Dhaka region [24]: AFM1 was detected in more than half (53/100) of the samples, and among these, 75% were above regulatory limits set in Europe for AFM1 in milk, and 43% exceeded the limit set for AFM1 in dairy milk of 500 ng/kg in Bangladesh [25]. The results of this study in the Dhaka region [24] warrant further analysis, including other regions of the country. Furthermore, the frequent presence of AFM1 in the urine of adults and children in Bangladesh [20,26,27] indicates the necessity of monitoring and screening food items that may be possible sources of aflatoxin exposure in the Bangladeshi population. Thus, the objective of our present study was to determine AFM1 concentrations in milk and milk products collected from four major divisional regions in Bangladesh, and to explore some variables which may affect contamination levels in raw milk.

2. Results

2.1. Levels of AFM1 in Milk and Milk Products

The detection frequencies and contamination levels of AFM1 in milk (raw, pasteurized, UHT) and in dairy products (milk powder, yogurt) are presented in Table 1 Overall, AFM1 was detected in 78.6% of all milk and milk products in the range from 5.0 to 198.7 ng/L (mean 51.5 ng/L). AFM1 was detected in 71.4% of raw milk samples (mean 41.1 ng/L, range 5.0–198.7 ng/L), and in 100% of pasteurized milk (mean 106 ng/L, range 17.2–187.7 ng/L) and UHT milk (mean 73 ng/L, range 12.2–146.9 ng/L). AFM1 contamination levels were comparatively lower in yogurt (mean 16.9 ng/L, range 8.3–41.1 ng/L) and milk powder samples (mean 6.6 ng/L, range 5.9–7.0 ng/L). In total, 32.4% of milk samples (raw milk 23.8%, pasteurized and UHT milk 73.3%) exceeded the EU regulatory limit (50 ng/kg) for AFM1 in milk. AFM1 contamination levels in all yogurt and milk powder samples were well below the EU regulatory limit.

2.2. Regional and Seasonal Variations in AFM1 Contamination Levels

AFM1 contamination levels were compared for the raw milk samples collected from the four divisional regions of Bangladesh. The lowest AFM1 contamination was observed in the Chittagong division (mean 6.6 ng/L, max 10.6 ng/L) compared to Sylhet (mean 53.7 ng/L, max 198.7 ng/L), Dhaka (mean 37.8 ng/L, max 97.2 ng/L), and Rajshahi (mean 34.8, max 131.4 ng/L) division (Table 2). The Sylhet region had the maximum amount of milk samples (32.5%) exceeding the EU regulatory limit, whereas all the samples from the Chittagong division were well below this limit.
Regarding seasonal variations, there was no significant difference at the level of AFM1 contamination between samples collected in the winter (mean 39.9 ng/L, max 115.5 ng/L) or summer (mean 41.6 ng/L, max 198.7 ng/L) period (Table 3). Yet, the percentage of milk samples that exceeded the EU regulatory limit was slightly higher in the summer (26.2%) than in the winter (20.5%) season.

3. Discussion

As AFM1 may pose a health risk for consumers of milk from dairy cattle fed with AFB1-contaminated feedstuffs, regulatory authorities have established limits for this mycotoxin metabolite (see Introduction). Regular monitoring of AFM1 is needed to control for compliance with food standards and protecting the population against hazardous dietary exposures. The prevalence and levels of AFM1 in milk and dairy products analyzed in various regions of the world (data reviewed by [1,8,28,29]) indicate a wide spectrum of human exposure to AFM1, with considerable differences between climate zones and countries. In the context of present work, there are studies on AFM1 occurrence in milk and milk products from South-Asian countries (listed in Table 4), which include recent ones from Bangladesh.
Regulation on mycotoxin contamination of major food categories, and limits for AFM1 in milk, were issued in 2017 in Bangladesh [25], yet data from related monitoring are recently emerging. The new data presented here on AFM1 in milk and milk products show a high prevalence of this contaminant (78.6%) in all samples collected in the four divisional regions in Bangladesh (Table 1). Detection frequency and mean levels of AFM1 in pasteurized and UHT milk were higher than those in raw milk, yet the concentration ranges observed in these sample types (5–198 ng/L) were similar. This observation is in accordance with findings that heat treatments in pasteurization and sterilization do not cause notable changes in AFM1 content in such products [1,8]. On the other hand, AFM1 contamination appeared to be lower in milk powder and yogurt (range 5.9–41.1 ng/L), but the sample numbers analyzed here preclude further conclusions on the effects of processing, although it has been found that AFM1 levels can decrease in yogurt production [14,45].
The higher number of raw milk samples allowed us to explore possible regional differences in AFM1 occurrence. The lowest AFM1 prevalence and levels were observed in milk from the Chittagong division (Table 2) with none of the samples exceeding the EU regulatory limit of 50 ng/kg. In contrast, in the Sylhet, Dhaka, and Rajshahi divisions, 32.5%, 28%, and 25%, respectively, of the collected milk had AFM1 levels exceeding this standard. Cattle feeding and grazing practices can vary between small farmers and commercial dairy farms. Small farmers are accustomed to open grazing practices of their cattle. In addition, they use stored feed such as dry rice paddy straw, rice husk, and wheat bran. Larger farms usually fully depend on stored feed such as dry rice paddy straw, rice husk, wheat bran, maize, different types of pulses, mustard oil cake, soybean bush, and, to a lesser extent, grass. Due to limited resources for this study, we could not collect background information on farming practices and dairy feeds (green fodder, dry fodder, and concentrate) provided at the different locations. Thus, we presently have no information on differences in AFM1 contamination. Yet, plans exist to investigate this aspect further, and to raise awareness on critical aspects such as proper storage of dairy feed and other relevant aspects in mycotoxin contamination [1,31].
Regarding seasonal variations, there was no significant difference in the levels of AFM1 contamination between the winter and summer period (Table 3), although the percentage of raw milk samples that exceeded the EU regulatory limit was slightly higher in the summer (26.2%) than in the winter (20.5%) season. Our findings differ from studies in Pakistan and India, at which the AFM1 levels in milk were often higher in the rainy/monsoon seasons and lower in the summer [31,36,46,47]. These outcomes are related to various factors, namely environmental conditions (high humidity) conducive for growth of Aspergillus in stored feed, and dairy animals getting more compound-feed in winter or more out-pasturing in summer. Yet, in these studies [31,36,46,47], the mean AFM1 milk levels in both seasons were high compared to those found in our study in Bangladesh.
Recently, Tarannum et al. [24] analyzed samples collected in the Dhaka district of Bangladesh and reported higher ranges and mean AFM1 concentrations in raw milk, while processed (pasteurized, UHT) milk had similar levels of contamination as observed in the present study (Table 4). Thus, both studies document frequent contamination of dairy milk in Bangladesh. Variations in AFM1 milk levels have been observed in other countries where multiple studies of this type have been conducted, as in Pakistan and India (Table 4). Such differences observed in the prevalence of AFM1 levels may depend on several factors, such as environmental conditions, different farming and feeding practices, and the quality and safety control system of the food business operators concordant with the legislations in force [1,31,36].
While the studies listed in Table 4 differ in milk types and dairy products covered, sample sizes, and methods used for AFM1 analysis, the reported outcomes show the frequent occurrence of the mycotoxin metabolite in food intended for human consumption in South Asia. AFM1 levels in a high percentage of samples were found to exceed the limit of 50 ng/kg set in the EU, and many samples crossed the 10-fold higher limit permitted in other regions of the world. In particular, the high AFM1 levels detected in infant formula in two studies in India [30,32] and in sweets in Pakistan [42] raise concerns. Thus, as these products are consumed by vulnerable parts of the population, they should be included in future sampling plans for AFM1 analysis in Bangladesh. AFM1 levels reported in dairy milk are higher than those determined recently in human breast milk (51.6%, mean 4.42 ng/L) in Bangladesh [48]. Thus, one can conclude that human breast milk is better and safer for infants than dairy milk.
In summary, the present study along with study [24] document the frequent presence of AFM1 in milk and milk products in Bangladesh, and at levels which often exceed regulatory standards for this toxic contaminant. Therefore, and to protect consumers against potential health risks from exposure to AFM1, more extensive and periodic control of AFM1 concentration in milk and dairy products is needed. AFM1 monitoring is relatively easy to perform, in contrast to an analysis of AFB1 contamination in animal feeds due to the heterogeneous distribution of the mycotoxin in raw materials and problems derived from sampling procedures [6]. As AFB1 contaminated feed is the source of AFM1 in milk, improved feed practices and proper storage conditions must be implemented to keep mycotoxin contamination in animal feedstuffs as low as possible.

4. Conclusions

AFM1 was frequently detected in milk and milk products collected in four regions of Bangladesh, and at levels that raise concern for the health of consumers, in particular for young children. Regular surveillance and monitoring are needed to prevent and control aflatoxin contamination in milk and milk products in Bangladesh. Furthermore, government agencies should train farmers by raising awareness of the toxicity of aflatoxins, of proper storage conditions of cattle feed to prevent mold growth, and encouraging further studies at dairy companies in order to reduce potential health risks and economic losses.

5. Materials and Methods

5.1. Study Areas and Sample Collection

This study was conducted at the Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet, Bangladesh. Between December 2018 and November 2019, a total of 145 cow milk and milk products were collected: raw milk samples were collected from four major divisional regions (Sylhet, Dhaka, Chittagong, and Rajshahi) of Bangladesh, whereas milk products were collected from the Sylhet and Dhaka regions. The samples were purchased to cover the following categories: raw milk (n = 105), fresh pasteurized milk (n = 15), ultra-high temperature (UHT)-treated milk (n = 15), fermented milk products such as yogurt (n = 5), and milk powder (n = 5). Raw milk samples were collected directly from farmers’ houses and dairy farms. Pasteurized milk, UHT-treated milk, and fermented milk products were bought from local retail shops; individual production and expiration dates, if provided, were recorded for the collected samples. Raw milk samples were collected in two seasonal periods (summer: March–October and winter: November–February) to check for variations in AFM1 contamination. Each category of milk samples was purchased in units of at least 250 mL. The collected samples were stored at −20 °C and analyzed within 2 months of collection.

5.2. Sample Preparation

Aflatoxin M1 concentration in milk and milk products was measured using a commercial enzyme-linked immunosorbent assay (ELISA) (Helica Biosystems Inc., Santa Ana, CA, USA, catalogue no. 961AFLM01M-96). The sample preparation was performed following the manufacturer’s instructions. Briefly, raw milk samples were placed at a refrigerated temperature overnight to initiate the coagulation of fat molecules. Pasteurized milk, UHT milk, and yogurt samples were refrigerated for 1–2 h. Then, all samples were centrifuged at 4000 rpm for 10 min at room temperature to induce separation of the upper fatty layer. For milk powder, about 10 g of sample was dissolved in 100 mL of distilled water and stirred with a magnetic stirrer for 5 min and centrifuged to separate the fat layer. The upper fatty layer was removed by aspiration, and the lower plasma layer of the milk was used in the assay.

5.3. Laboratory Analyses

Standard solutions and prepared samples (200 μL) were added to the precoated ELISA plates in duplicates and incubated for 2 h at ambient temperature. At the end of incubation, the contents of the wells were discarded, and the wells were washed three times with the washing buffer provided with the assay kit. After the washing steps, 100 μL of the conjugate was added to the wells and incubated for 15 min at room temperature. After incubation, the wells were washed and 100 μL of enzyme substrate was added to each well and incubated for 15 min. Following that, 100 μL of stop solution was added to each well and gently mixed. The absorbance of each microwell was measured at 450 nm by using an ELISA reader (Apollo 11 LB 913, Berthold, Germany) within 15 min. AFM1 concentration in each well was calculated using a semi-logarithmic standard curve (prepared using 0, 5, 10, 25, 50, and 100 pg/mL of AFM1 solutions), and the mean of the duplicates was used as the final result. To test the accuracy of the AFM1 estimations, recovery studies were performed by spiking skim milk samples with three different concentrations of AFM1 (5, 10, and 25 pg/mL). The repeatability at these three spike concentrations showed acceptable precisions for AFM1 measurements (Table 5). The recovery was 102%, 98%, and 94.8% in the spiked concentration, respectively. The detection limit (LOD) of this ELISA method was 5 pg/mL or 5 ng/L. Samples exceeding the signal for the highest AFM1 standard concentration (100 pg/mL) were further diluted and re-tested.

5.4. Statistical Analysis

All data were analyzed statistically using IBM SPSS Statistics version 23. Descriptive analysis was conducted to determine mean, median, and interquartile ranges of the analyte. The obtained data are presented as mean ± standard deviation, and ranges, frequency, and percentiles for the parameters. Differences in AFM1 concentrations between seasons and regions were analyzed by an independent sample t-test. One-way ANOVA was used to compare AFM1 concentrations in the four divisional regions in Bangladesh. A level of p-value < 0.05 was considered statistically significant.

Author Contributions

Conceptualization, N.A.; data curation, S.R., A.H.S. and F.I.; formal analysis, A.H.S. and F.I.; funding acquisition, F.I. and N.C.M.; investigation, N.A.; methodology, A.H.S., R.R.K., and N.H.M.; software, A.H.S.; supervision, N.A.; validation, G.H.D.; writing—original draft, N.A.; writing—review & editing, G.H.D. and N.A. All authors have read and agreed to the published version of the manuscript.

Funding

This study was funded by Shahjalal University of Science and Technology Research Centre (Project code: LS/2019/1/03).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Data are available from the corresponding authors upon reasonable request.

Acknowledgments

The authors are grateful to the grant LS/2019/1/03 from the Shahjalal University of Science and Technology Research Center.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Iqbal, S.Z.; Jinap, S.; Pirouz, A.A.; Faizal, A.A. Aflatoxin M1 in Milk and Dairy Products, Occurrence and Recent Challenges: A Review. Trends Food Sci. Technol. 2015, 46, 110–119. [Google Scholar] [CrossRef]
  2. Flores-Flores, M.E.; Lizarraga, E.; de Cerain, A.L.; González-Peñas, E. Presence of Mycotoxins in Animal Milk: A Review. Food Control 2015, 53, 163–176. [Google Scholar] [CrossRef]
  3. IARC International Agency for Research on Cancer. Aflatoxins. In Chemical Agents and Related Occupations; IARC Monogr Eval Carcinog Risk Chem Hum Vol 100F; IARC Press: Lyon, France, 2012; pp. 225–248. [Google Scholar]
  4. Britzi, M.; Friedman, S.; Miron, J.; Solomon, R.; Cuneah, O.; Shimshoni, J.; Soback, S.; Ashkenazi, R.; Armer, S.; Shlosberg, A. Carry-Over of Aflatoxin B1 to Aflatoxin M1 in High Yielding Israeli Cows in Mid- and Late-Lactation. Toxins 2013, 5, 173–183. [Google Scholar] [CrossRef]
  5. Gonçalves, B.L.; Gonçalves, J.L.; Rosim, R.E.; Cappato, L.P.; Cruz, A.G.; Oliveira, C.A.F.; Corassin, C.H. Effects of Different Sources of Saccharomyces Cerevisiae Biomass on Milk Production, Composition, and Aflatoxin M1 Excretion in Milk from Dairy Cows Fed Aflatoxin B1. J. Dairy Sci. 2017, 100, 5701–5708. [Google Scholar] [CrossRef] [Green Version]
  6. Rodríguez-Blanco, M.; Ramos, A.J.; Prim, M.; Sanchis, V.; Marín, S. Usefulness of the Analytical Control of Aflatoxins in Feedstuffs for Dairy Cows for the Prevention of Aflatoxin M 1 in Milk. Mycotoxin Res. 2020, 36, 11–22. [Google Scholar] [CrossRef]
  7. EFSA. Opinion of the Scientific Panel on Contaminants in the Food Chain [CONTAM] Related to Aflatoxin B1 as Undesirable Substance in Animal Feed. EFSA J. 2004, 39, 1–27. [Google Scholar] [CrossRef]
  8. Prandini, A.; Tansini, G.; Sigolo, S.; Filippi, L.; Laporta, M.; Piva, G. On the Occurrence of Aflatoxin M1 in Milk and Dairy Products. Food Chem. Toxicol. 2009, 47, 984–991. [Google Scholar] [CrossRef]
  9. EFSA. CONTAM Panel: Scientific Opinion—Risk Assessment of Aflatoxins in Food. EFSA J. 2020, 18, 6040. [Google Scholar] [CrossRef]
  10. Joint FAO/WHO Expert Committee on Food Additives World Health Organisation, Food and Agriculture Organisation of the United Nations and International Programme on Chemical Safety. In Evaluation of Certain Mycotoxins in Food: 56th Report of the Joint FAO/WHO Expert Committee on Food Additives; WHO Technical Report Series 906; WHO: Geneva, Switzerland, 2002.
  11. Joint FAO/WHO Expert Committee on Food Additives Safety Evaluation of Certain Mycotoxins in Food. Available online: Http://Www.Inchem.Org/Documents/Jecfa/Jecmono/V47je01.Htm (accessed on 6 June 2021).
  12. Campagnollo, F.B.; Ganev, K.C.; Khaneghah, A.M.; Portela, J.B.; Cruz, A.G.; Granato, D.; Corassin, C.H.; Oliveira, C.A.F.; Sant’Ana, A.S. The Occurrence and Effect of Unit Operations for Dairy Products Processing on the Fate of Aflatoxin M1: A Review. Food Control 2016, 68, 310–329. [Google Scholar] [CrossRef]
  13. Sherif, S.O.; Salama, E.E.; Abdel-Wahhab, M.A. Mycotoxins and Child Health: The Need for Health Risk Assessment. Int. J. Hyg. Environ. Health 2009, 212, 347–368. [Google Scholar] [CrossRef]
  14. Giovati, L.; Magliani, W.; Ciociola, T.; Santinoli, C.; Conti, S.; Polonelli, L. AFM1 in Milk: Physical, Biological, and Prophylactic Methods to Mitigate Contamination. Toxins 2015, 7, 4330–4349. [Google Scholar] [CrossRef]
  15. European Commission Commission Regulation (EC) No 1881/2006 of 19 December 2006 Setting Maximum Levels for Certain Contaminants in Foodstuffs. Off. J. Eur. Union 2006, 364, 5e24.
  16. van Egmond, H.P.; Schothorst, R.C.; Jonker, M.A. Regulations Relating to Mycotoxins in Food: Perspectives in a Global and European Context. Anal. Bioanal. Chem. 2007, 389, 147–157. [Google Scholar] [CrossRef] [Green Version]
  17. Streit, E.; Naehrer, K.; Rodrigues, I.; Schatzmayr, G. Mycotoxin Occurrence in Feed and Feed Raw Materials Worldwide: Long-Term Analysis with Special Focus on Europe and Asia. J. Sci. Food Agric. 2013, 93, 2892–2899. [Google Scholar] [CrossRef]
  18. Gruber-Dorninger, C.; Jenkins, T.; Schatzmayr, G. Global Mycotoxin Occurrence in Feed: A Ten-Year Survey. Toxins 2019, 11, 375. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  19. Ali, N.; Blaszkewicz, M.; Alim, A.; Hossain, K.; Degen, G.H. Urinary Biomarkers of Ochratoxin A and Citrinin Exposure in Two Bangladeshi Cohorts: Follow-up Study on Regional and Seasonal Influences. Arch. Toxicol. 2016, 90, 2683–2697. [Google Scholar] [CrossRef] [PubMed]
  20. Ali, N.; Blaszkewicz, M.; Hossain, K.; Degen, G.H. Determination of Aflatoxin M1 in Urine Samples Indicates Frequent Dietary Exposure to Aflatoxin B1 in the Bangladeshi Population. Int. J. Hyg. Environ. Health 2017, 220, 271–281. [Google Scholar] [CrossRef]
  21. Bhuiyan, M.N.H.; Hassan, M.T.; Begum, M.; Ahan, M.; Rahim, M. Occurrence and Seasonal Trends of Aflatoxin in Rice, Maize and Wheat in Bangladesh. Int. J. Sustain. Agric. Technol. 2013, 9, 8–14. [Google Scholar]
  22. Roy, M.; Harris, J.; Afreen, S.; Deak, E.; Gade, L.; Balajee, S.A.; Park, B.; Chiller, T.; Luby, S. Aflatoxin Contamination in Food Commodities in Bangladesh. Food Addit. Contam. Part B 2013, 6, 17–23. [Google Scholar] [CrossRef]
  23. Huque, K.; Sarker, N. Feeds and Feeding of Livestock in Bangladesh: Performance, Constraints and Options Forward. Bang. J. Anim. Sci. 2014, 43, 1–10. [Google Scholar] [CrossRef] [Green Version]
  24. Tarannum, N.; Nipa, M.N.; Das, S.; Parveen, S. Aflatoxin M1 Detection by ELISA in Raw and Processed Milk in Bangladesh. Toxicol. Rep. 2020, 7, 1339–1343. [Google Scholar] [CrossRef]
  25. BFSA Bangladesh Food Safety Authority. Food Safety Regulations. Available online: Http://Www.Bfsa.Gov.Bd/Sites/Default/Files/Files/Bfsa.Portal.Gov.Bd/Law/81690675_9a69_483b_8915_1fe8f6b2ddd0/Food-Safety-(Contaminants,-Toxins-and-Harmful-Residues)-Regulations,-2017.Pdf (accessed on 18 February 2021).
  26. Ali, N.; Hossain, K.; Blaszkewicz, M.; Rahman, M.; Mohanto, N.C.; Alim, A.; Degen, G.H. Occurrence of Aflatoxin M1 in Urines from Rural and Urban Adult Cohorts in Bangladesh. Arch. Toxicol. 2016, 90, 1749–1755. [Google Scholar] [CrossRef] [PubMed]
  27. Ali, N.; Manirujjaman, M.; Rana, S.; Degen, G.H. Determination of Aflatoxin M1 and Deoxynivalenol Biomarkers in Infants and Children Urines from Bangladesh. Arch. Toxicol. 2020, 94, 3775–3786. [Google Scholar] [CrossRef] [PubMed]
  28. Womack, E.D.; Sparks, D.L.; Brown, A.E. Aflatoxin M1 in Milk and Milk Products: A Short Review. World Mycotoxin J. 2016, 9, 305–315. [Google Scholar] [CrossRef]
  29. Rahmani, J.; Alipour, S.; Miri, A.; Fakhri, Y.; Riahi, S.-M.; Keramati, H.; Moradi, M.; Amanidaz, N.; Pouya, R.H.; Bahmani, Z.; et al. The Prevalence of Aflatoxin M1 in Milk of Middle East Region: A Systematic Review, Meta-Analysis and Probabilistic Health Risk Assessment. Food Chem. Toxicol. 2018, 118, 653–666. [Google Scholar] [CrossRef] [PubMed]
  30. Rastogi, S.; Dwivedi, P.D.; Khanna, S.K.; Das, M. Detection of Aflatoxin M1 Contamination in Milk and Infant Milk Products from Indian Markets by ELISA. Food Control 2004, 15, 287–290. [Google Scholar] [CrossRef]
  31. Patyal, A.; Gill, J.P.S.; Bedi, J.S.; Aulakh, R.S. Potential Risk Factors Associated with the Occurrence of Aflatoxin M1 in Raw Milk Produced under Different Farm Conditions. J. Environ. Sci. Health Part B 2020, 55, 827–834. [Google Scholar] [CrossRef]
  32. Kanungo, L.; Bhand, S. A Survey of Aflatoxin M1 in Some Commercial Milk Samples and Infant Formula Milk Samples in Goa, India. Food Agric. Immunol. 2014, 25, 467–476. [Google Scholar] [CrossRef]
  33. Siddappa, V.; Nanjegowda, D.K.; Viswanath, P. Occurrence of Aflatoxin M1 in Some Samples of UHT, Raw & Pasteurized Milk from Indian States of Karnataka and Tamilnadu. Food Chem. Toxicol. 2012, 50, 4158–4162. [Google Scholar] [CrossRef]
  34. Nile, S.H.; Park, S.W.; Khobragade, C.N. Occurrence and Analysis of Aflatoxin M1 in Milk Produced by Indian Dairy Species. Food Agric. Immunol. 2016, 27, 358–366. [Google Scholar] [CrossRef] [Green Version]
  35. Kafle, P.; Sedai, D.; Rai, K.P.; Pokharel, B.B. Study on the Level of Aflatoxin M1 Contamination in Raw and Processed Milk Marketed in Kathmandu Valley. J. Food Sci. Technol. Nepal 2012, 7, 52–56. [Google Scholar] [CrossRef] [Green Version]
  36. Hussain, I.; Anwar, J. A Study on Contamination of Aflatoxin M1 in Raw Milk in the Punjab Province of Pakistan. Food Control 2008, 19, 393–395. [Google Scholar] [CrossRef]
  37. Yunus, A.; Imtiaz, N.; Khan, H.; Ibrahim, M.; Zafar, Y. Aflatoxin Contamination of Milk Marketed in Pakistan: A Longitudinal Study. Toxins 2019, 11, 110. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  38. Asghar, M.A.; Ahmed, A.; Asghar, M.A. Aflatoxin M1 in Fresh Milk Collected from Local Markets of Karachi, Pakistan. Food Addit. Contam. Part B 2018, 11, 167–174. [Google Scholar] [CrossRef]
  39. Ahmad, M.; Awais, M.; Ali, S.W.; Ali Khan, H.A.; Riaz, M.; Sultan, A.; Shakeel Bashir, M.; Ishtiaq Chaudhry, A. Occurrence of Aflatoxin M1 in Raw and Processed Milk and Assessment of Daily Intake in Lahore, Multan Cities of Pakistan. Food Addit. Contam. Part B 2019, 12, 18–23. [Google Scholar] [CrossRef] [PubMed]
  40. Iqbal, S.Z.; Asi, M.R. Assessment of Aflatoxin M1 in Milk and Milk Products from Punjab, Pakistan. Food Control 2013, 30, 235–239. [Google Scholar] [CrossRef]
  41. Iqbal, S.Z.; Asi, M.R.; Selamat, J. Aflatoxin M 1 in Milk from Urban and Rural Farmhouses of Punjab, Pakistan. Food Addit. Contam. Part B 2014, 7, 17–20. [Google Scholar] [CrossRef]
  42. Sadia, A.; Jabbar, M.A.; Deng, Y.; Hussain, E.A.; Riffat, S.; Naveed, S.; Arif, M. A Survey of Aflatoxin M1 in Milk and Sweets of Punjab, Pakistan. Food Control 2012, 26, 235–240. [Google Scholar] [CrossRef]
  43. Maqbool, U.; Anwar-Ul-Haq; Ahmad, M. ELISA Determination of Aflatoxin M 1 in Milk and Dairy Products in Pakistan. Toxicol. Environ. Chem. 2009, 91, 241–249. [Google Scholar] [CrossRef]
  44. Pathirana, U.; Wimalasiri, K.; Silva, K.; Gunarathne, S. Investigation of Farm Gate Cow Milk for Aflatoxin M1. Trop. Agric. Res. 2010, 21, 119. [Google Scholar] [CrossRef] [Green Version]
  45. Govaris, A.; Roussi, V.; Koidis, P.A.; Botsoglou, N.A. Distribution and Stability of Aflatoxin M1 during Production and Storage of Yoghurt. Food Addit. Contam. 2002, 19, 1043–1050. [Google Scholar] [CrossRef]
  46. Aslam, N.; Tipu, M.Y.; Ishaq, M.; Cowling, A.; McGill, D.; Warriach, H.M.; Wynn, P. Higher Levels of Aflatoxin M1 Contamination and Poorer Composition of Milk Supplied by Informal Milk Marketing Chains in Pakistan. Toxins 2016, 8, 347. [Google Scholar] [CrossRef] [PubMed]
  47. Iqbal, S.Z.; Asi, M.R.; Jinap, S. Variation of Aflatoxin M1 Contamination in Milk and Milk Products Collected during Winter and Summer Seasons. Food Control 2013, 34, 714–718. [Google Scholar] [CrossRef]
  48. Islam, F.; Trisha, A.D.; Hafsa, J.M.; Degen, G.H.; Ali, N. Occurrence of aflatoxin M1 in human breast milk in Bangladesh. Mycotoxin Res. 2021. [Google Scholar] [CrossRef] [PubMed]
Table
Table 1. Occurrence and levels of AFM1 in milk and milk products in Bangladesh.
Table 1. Occurrence and levels of AFM1 in milk and milk products in Bangladesh.
SamplesNPositive
Samples, n (%)		Mean ± SD
(ng/L)		Median (Range)
(ng/L)		Above 50 ng/L
n (%)
Raw milk10575 (71.4)41.1 ± 43.4 a23.5 (5.0–198.7)25 (23.8)
Pasteurized milk1515 (100)106.0 ± 58.5 b115.7 (17.2–187.7)11 (73.3)
UHT milk1515 (100)73.0 ± 42.0 c74.0 (12.2–146.9)11 (73.3)
Milk powder54 (80)6.6 ± 0.56.7 (5.9–7.0)0
Yogurt55 (100)16.9 ± 13.612.1 (8.3–41.1)0
Total145114 (78.6)51.5 ± 50.427.9 (5.0–198.7)47 (32.4)
Only positive samples (≥LOD; 5 ng/L) were considered during mean and median calculation. a When raw milk is compared to milk powder (p < 0.001), b when pasteurized milk is compared to raw milk (p < 0.01), yogurt, and milk powder (p < 0.001), c when UHT milk is compared to yogurt (p < 0.01) and milk powder (p < 0.001). p-values were obtained from one-way ANOVA.
Table
Table 2. Levels of AFM1 in raw milk samples collected from four divisional regions.
Table 2. Levels of AFM1 in raw milk samples collected from four divisional regions.
RegionsnPositive Sample
n (%)		Mean ±SD
(ng/L)		Median (Range)
(ng/L)		Above 50 ng/L
n (%)
Sylhet4034 (85)53.7 ± 51.034.0 (5.5–198.7)13 (32.5)
Dhaka2515 (60)37.8 ± 29.233.2 (5.1–97.2)7 (28.0)
Chittagong208 (40)6.6 ± 1.9 *5.8 (5.0–10.6)0 (0)
Rajshahi2018 (90)34.8 ± 39.119.2 (6.3–131.4)5 (25)
Total10575 (71.4)41.0 ± 43.423.5 (5.0–198.7)25 (23.8)
Only positive samples (≥LOD: 5 ng/L) were considered during mean and median calculation. * When comparing mean AFM1 concentration to Sylhet (p < 0.001), Dhaka (p < 0.01) and Rajshahi (p < 0.05) regions. p-values were obtained from one-way ANOVA.
Table
Table 3. Levels of AFM1 in raw milk samples collected in winter and summer seasons.
Table 3. Levels of AFM1 in raw milk samples collected in winter and summer seasons.
SeasonnPositive Sample n (%)Mean ± SD
(ng/L)		Median (Range)
(ng/L)		Above 50 ng/L
n (%)
Winter4428 (63.6)39.9 ± 34.327.9 (5.5–115.5)9 (20.5)
Summer6147 (77.1)41.6 ± 43.416.5 (5.0–198.7)16 (26.2)
Total10575 (71.4)41.0 ± 43.423.5 (5.0–198.7)25 (23.8)
Only positive samples (≥LOD: 5 ng/L) were considered during mean and median calculation.
Table
Table 4. Occurrence and levels of AFM1 in milk and dairy products in South-Asian countries.
Table 4. Occurrence and levels of AFM1 in milk and dairy products in South-Asian countries.
CountrySample TypePositive Samples
n (%)		Mean
(ng/L)		Range
(ng/L)		Samples ≥ 50 ng/L
n (%)		Analytical MethodLOD/LOQ
ng/L		References
BangladeshRaw Milk75/105 (71.4)41.15–198.725 (23.8)ELISA (SKM)5.0/-Present study
Pasteurized milk15/15 (100)10617.2–187.711 (73.3)
UHT milk15/15 (100)7312.2–146.911 (73.3)
Milk powder4/5 (80)6.65.9–7.00
Yogurt5/5 (100)16.98.3–41.10
BangladeshRaw milk35/50 (70.0)699.122.8–1489.334 (97.0)ELISA (SKM)18.0/NI[24]
Pasteurized milk13/25 (52.0)99.818.1–672.26 (46.0)
UHT milk05/25 (20.0)35.525.1–49.00
IndiaIMF17/17 (100.0)350.077.0–844.017 (100.0)ELISA (SKM)NI[30]
Infant formula17/18 (94.0)326.0143.0–770.017 (100.0)
MBCWF38/40 (95.0)267.065.0–1012.038 (100.0)
Liquid milk4/12 (33.0)86.028.0–164.03 (75.0)
IndiaRaw milk110/189 (58.0)917.07.0–13,100.096 (50.8)ELISA (SKM)5.0/NI[31]
IndiaInfant formula18/18 (100.0)NI150.0–713.018 (100.0)ELISA (SKM)5.0/NI[32]
Liquid milk54/54 (100.0)NI172.0–820.054 (100.0)
IndiaUHT milk30/45 (66.6)NINI29 (96.7)HPLC (IAC)4.0/NI[33]
Raw milk3/7 (42.9)NININI
Pasteurized milk29/45 (42.9)NINI3 (10.3)
IndiaBuffalo milk58/150 (38.6)26.0NI9 (16.0)ELISA (SKM) HPLC (IAC)5.0/NI (ELISA)[34]
Cow milk68/150 (45.3)18.0NI30 (44.0)
Goat milk50/150 (33.3)14.0NI5 (10.0)
Sheep milk55/150 (36.6)17.0NI7 (12.0)
Cheese2/2 (100.0)NINI0
NepalRaw milk8/16 (50.0)30.026.0–138.04 (25.0)TLCNI[35]
Pasteurized milk6/16 (37.5)22.025.0–127.03 (18.8)
PakistanRaw milk168/168 (100)199–50310–700167 (99.4)Fluorometric (IAC)NI[36]
PakistanUHT milk85 (NI)254.9ND-1536.080 (94.1)ELISA (SKM)4.4/NI[37]
Pasteurized milk78/78 (100.0)939.532.8–4808.074 (95.0)
Raw milk55/55 (100.0)1535.01912.0–7460.755 (100.0)
PakistanMilk143/156 (91.7)346.220.0–3090.0125 (80.1)ELISA (SKM)5.0/NI[38]
PakistanRaw milk137/150 (91.3)198.86.0–554.0108 (72.0)ELISA (SKM)4.3/5.0[39]
TW30/30 (100.0)113.013.0–257.017 (56.0)
UHT milk30/30 (100.0)164.010.0–345.020 (66.0)
PakistanMilk76/107 (71)150.14.0–845.444 (58.0)HPLC (IAC)4.0/NI[40]
Yogurt59/96 (61.0)94.44.0–615.828 (47.0)
Butter33/74 (45.0)69.74.0–413.417 (52.0)
PakistanRaw milk63/107 (59.0)55.0<4.0–980.038 (35.5)HPLC-FLD (IAC)4.0/12.0[41]
PakistanMilk76/107 (71)212.24–845.444 (58)HPLC-FLD (IAC)4/NI[40]
Yogurt59/96 (61)147.14–615.828 (47)
White Cheese93/119 (78)189.14–595.414 (15)
Cheese cream89/150 (59)172.94–456.310 (11)
Butter33/74 (45)156.34–413.417 (52)
PakistanShop milk137/175 (78.0)176.02.0–1600.050 (28.6)ELISA (SKM)2.0/NI[42]
Household milk25/40 (62.0)470.03.0–1900.018 (45.0)
Farm milk15/17 (88.0)110.02.0–794.07(41.0)
Sweets134/138 (97.0)480.010.0–1500.0108 (78.0) 10.0/NI
PakistanMilk18/21 (85.7)NI11.0–14.00ELISA (SKM)NI[43]
Yogurt6/10 (60.0)NI10.0–13.0
Butter4/10 (40.0)NI7.0–7.4
Sri LankaMilk29/87 (33.3)40.213.1–84.58 (9.2)HPLC (IAC)10.0/NI[44]
NI: not indicated, UHT: ultra-high temperature, IMF: infant milk food, MBCWF: milk based cereal weaning food, TW: tea whitener, ELISA: enzyme-linked immunosorbent assay, HPLC-FLD: high pressure liquid chromatography with fluorescence detection, SKM: skim milk, IAC: immunoaffinity columns. Note: for cheese, EU limit is 250 ng/kg.
Table
Table 5. The assay repeatability for AFM1 in spiked milk samples.
Table 5. The assay repeatability for AFM1 in spiked milk samples.
Spike Level (ng/L)Repeats (n)Mean ± SD (ng/L)Recovery (%)RSD (%)
555.1 ± 0.3102.05.9
1059.8 ± 0.298.02.4
25523.7 ± 0.694.82.5
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Sumon, A.H.; Islam, F.; Mohanto, N.C.; Kathak, R.R.; Molla, N.H.; Rana, S.; Degen, G.H.; Ali, N. The Presence of Aflatoxin M1 in Milk and Milk Products in Bangladesh. Toxins 2021, 13, 440. https://doi.org/10.3390/toxins13070440

AMA Style

Sumon AH, Islam F, Mohanto NC, Kathak RR, Molla NH, Rana S, Degen GH, Ali N. The Presence of Aflatoxin M1 in Milk and Milk Products in Bangladesh. Toxins. 2021; 13(7):440. https://doi.org/10.3390/toxins13070440

Chicago/Turabian Style

Sumon, Abu Hasan, Farjana Islam, Nayan Chandra Mohanto, Rahanuma Raihanu Kathak, Noyan Hossain Molla, Sohel Rana, Gisela H. Degen, and Nurshad Ali. 2021. "The Presence of Aflatoxin M1 in Milk and Milk Products in Bangladesh" Toxins 13, no. 7: 440. https://doi.org/10.3390/toxins13070440

APA Style

Sumon, A. H., Islam, F., Mohanto, N. C., Kathak, R. R., Molla, N. H., Rana, S., Degen, G. H., & Ali, N. (2021). The Presence of Aflatoxin M1 in Milk and Milk Products in Bangladesh. Toxins, 13(7), 440. https://doi.org/10.3390/toxins13070440

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop