AgTx2-GFP, Fluorescent Blocker Targeting Pharmacologically Important Kv1.x (x = 1, 3, 6) Channels
Abstract
:1. Introduction
2. Results and Discussion
2.1. Interactions of AgTx2-GFP with KcsA-Kv1.x (x = 1, 3, 6) Channels
2.2. Electrophysiological Studies of AgTx2-GFP
2.3. Interaction of AgTx2-GFP with Kv1.3 on Mammalian Cell Membranes
3. Conclusions
4. Materials and Methods
4.1. Reagents
4.2. Experiments with KcsA and KcsA-Kv1.x (x = 1, 3, 6) Channels
4.3. Electrophysiological Experiments
4.4. Experiments with Neuro2a Cells Expressing Kv1.3 Channels
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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pH | KcsA-Kv1.1 | KcsA-Kv1.3 | KcsA-Kv1.6 |
---|---|---|---|
7.0 | 23 ± 4 | 0.14 ± 0.02 | 0.60 ± 0.06 |
7.5 | 15 ± 3 * | 0.10 ± 0.01 * | 0.30 ± 0.08 * |
8.0 | 12 ± 2 * | 0.25 ± 0.02 * | 1.3 ± 0.4 * |
Toxin | KcsA-Kv1.1 | KcsA-Kv1.3 | KcsA-Kv1.6 |
---|---|---|---|
AgTx2 | 3.7 ± 0.8 | 0.039 ± 0.007 | 0.027 ± 0.004 |
KTx1 | 3.8 ± 0.8 | 0.032 ± 0.006 | 0.016 ± 0.002 |
Ligand | Kv1.1 | Kv1.2 | Kv1.3 | Kv1.6 |
---|---|---|---|---|
AgTx2-GFP | 3.5 ± 0.5 1 | - 2 | 2.31 ± 0.22 | 1.30 ± 0.15 |
AgTx2 | 4.2 ± 0.3 | 34 ± 6 | 0.17 ± 0.03 | 0.60 ± 0.11 |
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Primak, A.L.; Orlov, N.A.; Peigneur, S.; Tytgat, J.; Ignatova, A.A.; Denisova, K.R.; Yakimov, S.A.; Kirpichnikov, M.P.; Nekrasova, O.V.; Feofanov, A.V. AgTx2-GFP, Fluorescent Blocker Targeting Pharmacologically Important Kv1.x (x = 1, 3, 6) Channels. Toxins 2023, 15, 229. https://doi.org/10.3390/toxins15030229
Primak AL, Orlov NA, Peigneur S, Tytgat J, Ignatova AA, Denisova KR, Yakimov SA, Kirpichnikov MP, Nekrasova OV, Feofanov AV. AgTx2-GFP, Fluorescent Blocker Targeting Pharmacologically Important Kv1.x (x = 1, 3, 6) Channels. Toxins. 2023; 15(3):229. https://doi.org/10.3390/toxins15030229
Chicago/Turabian StylePrimak, Alexandra L., Nikita A. Orlov, Steve Peigneur, Jan Tytgat, Anastasia A. Ignatova, Kristina R. Denisova, Sergey A. Yakimov, Mikhail P. Kirpichnikov, Oksana V. Nekrasova, and Alexey V. Feofanov. 2023. "AgTx2-GFP, Fluorescent Blocker Targeting Pharmacologically Important Kv1.x (x = 1, 3, 6) Channels" Toxins 15, no. 3: 229. https://doi.org/10.3390/toxins15030229