Dimethylarginine Dimethylaminohydrolase/Nitric Oxide Synthase Pathway in Liver and Kidney: Protective Effect of Cyanidin 3-O-β-D-Glucoside on Ochratoxin-A Toxicity
Abstract
:1. Introduction
2. Material and Methods
2.1. Chemicals
2.2. Animals and Treatment
2.3. Western Blot Analysis
2.4. NO2−/NO3− Quantification
2.5. DDAH Enzyme Activity Assay
2.6. Statistical Analyses
3. Results
Kikney | C | C3G | O | O + C3G |
---|---|---|---|---|
DDAH activity | 3.59 ± 0.03 | 3.58 ± 0.05 | 5.31 ± 0.04 * | 2.93 ± 0.01 ** |
nitrite/nitrate levels | 5.15 ± 0.04 | 2.01 ± 0.02 * | 9.37 ± 0.09 * | 6.34 ± 0.08 ** |
Liver | C | C3G | O | O + C3G |
---|---|---|---|---|
DDAH activity | 1.74 ± 0.01 | 1.58 ± 0.03 | 1.50 ± 0.01 | 1.41 ± 0.02 |
nitrite/nitrate levels | 6.86 ± 0.09 | 3.14 ± 0.02 * | 11.42 ± 0.10 * | 7.10 ± 0.07 ** |
5. Discussion
References
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Sorrenti, V.; Di Giacomo, C.; Acquaviva, R.; Bognanno, M.; Grilli, E.; D’Orazio, N.; Galvano, F. Dimethylarginine Dimethylaminohydrolase/Nitric Oxide Synthase Pathway in Liver and Kidney: Protective Effect of Cyanidin 3-O-β-D-Glucoside on Ochratoxin-A Toxicity. Toxins 2012, 4, 353-363. https://doi.org/10.3390/toxins4050353
Sorrenti V, Di Giacomo C, Acquaviva R, Bognanno M, Grilli E, D’Orazio N, Galvano F. Dimethylarginine Dimethylaminohydrolase/Nitric Oxide Synthase Pathway in Liver and Kidney: Protective Effect of Cyanidin 3-O-β-D-Glucoside on Ochratoxin-A Toxicity. Toxins. 2012; 4(5):353-363. https://doi.org/10.3390/toxins4050353
Chicago/Turabian StyleSorrenti, Valeria, Claudia Di Giacomo, Rosaria Acquaviva, Matteo Bognanno, Ester Grilli, Nicolantonio D’Orazio, and Fabio Galvano. 2012. "Dimethylarginine Dimethylaminohydrolase/Nitric Oxide Synthase Pathway in Liver and Kidney: Protective Effect of Cyanidin 3-O-β-D-Glucoside on Ochratoxin-A Toxicity" Toxins 4, no. 5: 353-363. https://doi.org/10.3390/toxins4050353