Improvements of Microcontact Printing for Micropatterned Cell Growth by Contrast Enhancement
Abstract
:1. Introduction
2. Materials and Methods
2.1. Stamp Fabrication
2.2. Chemical Vapor Deposition (CVD) of Silanes
2.3. Heat Treatment
2.4. Microcontact Printing
2.5. Capillary Deposition of MnO2
2.6. Contact Angle Measurements
2.7. Light Microscopy
2.8. Cell Culture
2.9. Live-Dead Stainings
3. Results and Discussion
3.1. Background Toxification via Water-Insoluble MnO2
3.2. Heat-Induced Background Hydrophobization of Glass
3.3. Glymo Silanization for Covalent Bonding of Amino Acids on a Cell-Repellent Background
3.3.1. Glymo-Functionalized Glass Improves the Contrast between Pattern and Background
3.3.2. Glymo Is Long-Term Stable on Glass
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Hondrich, T.J.J.; Deußen, O.; Grannemann, C.; Brinkmann, D.; Offenhäusser, A. Improvements of Microcontact Printing for Micropatterned Cell Growth by Contrast Enhancement. Micromachines 2019, 10, 659. https://doi.org/10.3390/mi10100659
Hondrich TJJ, Deußen O, Grannemann C, Brinkmann D, Offenhäusser A. Improvements of Microcontact Printing for Micropatterned Cell Growth by Contrast Enhancement. Micromachines. 2019; 10(10):659. https://doi.org/10.3390/mi10100659
Chicago/Turabian StyleHondrich, Timm J. J., Oliver Deußen, Caroline Grannemann, Dominik Brinkmann, and Andreas Offenhäusser. 2019. "Improvements of Microcontact Printing for Micropatterned Cell Growth by Contrast Enhancement" Micromachines 10, no. 10: 659. https://doi.org/10.3390/mi10100659